CAPÍTULO 2 CARACTERÍSTICAS TÉCNICAS Y COSTOS REFERENCIALES DE LOS
2.3 EL ESTÁNDAR H.323
2.3.1 COMPONENTES H.323
2.3.1.4 Gatekeeper (Guardián de Puerta)
2.3.1.4.1 Procedimiento de Conexión
In this thesis, I have shown that there is an IPg-dependent calcium release mechanism that controls passage into mitosis in the first mitotic cell
division of the sea urchin embryo. The most important piece of data supporting this hypothesis is that I can improve the detection frequency of these transients
by using confocal microscopy and dextran-conjugates of calcium-sensitive dyes. The fact that the detection frequency can be improved suggests that the
limitations of calcium detection systems is the limiting factor in the study of these transients. In support of this hypothesis, ratiometric images reveal calmodulin signals prior to NEB, and through mitosis.
If calcium transients are the trigger for mitosis entry, these must interact in some way with cell cycle control proteins. My results suggest just this fact, the calcium release machinery appears to be influenced by synthesis of the cyclins,
which tightly regulate the correct timing of calcium transients to the correct point within the cell cycle. However, the fact that calcium oscillations still occur when cyclin synthesis is inhibited suggests that calcium release is somewhat independent of the cell cycle control proteins, but may cooperate with the cdc2
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