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A rapid multiplex assay for nucleic acid-based diagnostics

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Figure

Fig. 1. A depiction of the essential components of an MOL-PCR assay. Ligation is achieved by using synthetic nucleic acids, multiplex oligonucleotides (MOLigo pairs)
Fig. 2. A 10-plex assay to screen for in-silico downselected MOLigo pairs for the detection of B
Fig. 4. A): The ability of MOL-PCR to identify the base present at a SNP site was demonstrated by performing the assay with four different MOLigo1's, each one containing a complimentary base to one of the possible SNP sequences
Fig. 5. A 13-plex assay for screening 3 biothreat agents (B. anthracis, Y .pestis, and F

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