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Validation of a high performance liquid chromatographic method for quantitative determination of boldine in fluid extract of boldo

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829 ISSN 0326-2383

KEY WORDS:Boldine, High-performance liquid chromatography, Peumus boldusMol., Quality control, Validation.

* Author to whom correspondence should be addressed. E-mail: [email protected]

Latin American Journal of Pharmacy

(formerly

Acta Farmacéutica Bonaerense)

Lat. Am. J. Pharm. 30

(4): 829-32 (2011)

Short communication Received: September 22, 2010 Revised version: October 13, 2010 Accepted: October 14, 2010

Validation of a High Performance Liquid Chromatographic Method

for Quantitative Determination of Boldine in Fluid Extract of Boldo

Francieli CASAL

1

, Michele MALLMANN

2

, Helen C. PEDRONI

2

,

Manoela M. GRAZZIOTIN

2

& Leandro TASSO *

1

1

Curso de Farmácia, Universidade de Caxias do Sul, Caxias do Sul - RS, Brazil.

2

Laboratório Saúde Ltda, Porto Alegre - RS, Brazil.

SUMMARY. A simple and specific method was validated for quantification of boldine in fluid extract of boldo (Peumus boldusMol.) using high-performance liquid chromatography. A reversed-phase C18,

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