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Antiviral mode of action of bovine dialyzable leukocyte extract against human immunodeficiency virus type 1 infection

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Academic year: 2020

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Figure 1 Cytotoxicity assessment of bDLE and HIV-1 inhibition activity. A) HeLa-CD4-LTR- b-gal cells (5 × 10 4 cells/well) and B) HIV-1 IIIB  cell-free viruses (MOI 0.2-0.5) were challenged with two-fold serial dilutions of bDLE
Figure 3 Inhibition of Env/CD4-mediated membrane fusion. b-gal activity was measured after CD4 cells and Env cells were co-cultured after exposure to A) bDLE, B) UC781 and C) T-20 under different circumstances: ( ■) CD4 cells were exposed to the compound a
Figure 4 Time of intervention in HIV-1 life cycle. HeLa/CD4-LTR- b-gal cells were infected with HIV-1 IIIB cell-free virus before A) bDLE (2 IU), B) T-20 (100 μM), C) UC781 (70 nM), D) 118-D-24 (120 μM) and E) Amprenavir (0.1 mM), were added upon HIV-1 ino

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