CRISPR Cas9: técnicas y aplicaciones

competed against other partners, fluorescent protein reassembly will be different and thus, also changes in fluorescence signal could be detected (Figure 2.6). Moreover, we have

Por otro lado, al igual que en el estudio de Lu et al, se realizó una disrupción del gen PDCD1 (PD-1). Por otro lado, se modificó el receptor TCR para que se dirigiera

Cell viability after shipment was > 80% in all cases, enabling evaluation of (1) adhesion to plastic flasks and hydroxyapatite tricalcium phosphate osteoconductive

I grab the opportunity to thank my previous and current lab mates at CNIC for giving me memorable moments and being a constant source of help.. Last but not the least I would

Protein extracts were prepared from Arabidopsis cell suspension culture protoplasts expressing either HA-tagged wt CAH1 (HC), in presence or absence of tunicamycin (Tun), or

This technique efficiently allowed the release of Nrf2 for its nuclear translocation: higher levels of nuclear Nrf2 were detected under basal conditions and after 4 and 8 hours of

Para llevar a cabo las deleciones del genoma se construyeron 3 plásmidos recombinantes diferentes, con idéntica región de homología 3’-DOWN, siendo variables las regiones

Mediante el sistema CRISPR-Cas9 se han conseguido ya cerdos modelo para distintas enfermedades y síndromes humanos ( Fig. 6 ), como por ejemplo la enfermedad de Niemman- Pick de tipo