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Full characterization of the small RNA transcriptome using novel computational methods for high-throughput sequencing data: study of miRNA variability in eukaryote organisms.

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Academic year: 2017

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Figure 1.1: MiRNA biogenesis (Kim et algenes are transcribed by RNA polymerase II to generate the primary transcripts (pri-miRNAs), which will be cropped by the Drosha generating 65 nucleotide (nt) pre-miRNAs, then recognized by the nuclear export factor e
Figure 1.2: Type of miRNA target sites from Bartel et al7-9 nt seed matched seed.Sites with productive 3’ pairing
Figure 1.3:Mechanisms of miRNA-mediated gene silencing in animalsbinding protein) and 3’ poly(A) tail and translational initiation factor (eIF4G) and cap-binding protein
Figure 1.4:Amplification loop consisting of Piwi/Aub complexes,Potential sources of primary piRNAs are piRNA cluster transcripts and maternallyinherited piRNA complexes with a nt bias at position 1 of Uracil.Piwi/Aub and piRNA targets the transcript of active transposon and cleavages it at apoint ten nucleotides from the 5’-end of the primary piRNA, producing the secondarypiRNA, which is loaded into Ago3.transcript and cleavage at the Adenine nucleotide, generating a primary piRNA whichAgo3complexes, piRNA cluster transcripts, and transcripts of active transposons(Brennecked et al, 2007).Nucleotide cleavage events are shown as scissors.The complex ofThis secondary piRNA pairs to a piRNA clusterwill be load into Piwi/Aub proteins closing the cycle.
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