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Real time PCR Application in dengue studies

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Figure 4. A. Amplification curves from six different amounts of plasmid pGEM-DV2core. It is noted that each one reaches the threshold at a different cycle, being the largest concentration samples (sample 7 corresponding to 10 7  copies/ml) the sooner they
Figure 5. Absolute quantification. Comparison of the number of viral copies obtained from peritoneal macrophages supernatants infected with DENV at MOI 0,1 (*p< 0,01)
Figure 7. Relative quantification. Amount of viral RNA copies obtained from monolayers of peritoneal macrophages of BALB/c and ICR mice infected with DENV

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