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Site specific cleavage of ribosomal RNA in Escherichia coli based cell free protein synthesis systems

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Academic year: 2020

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Figure

Fig 1. Experimental setup for investigation of the in vitro translation machinery. (A) To measure elongation factors from the cell-free extract, samples were mixed with an isotope-labeled cell-extract, which served as an internal reference.
Fig 2. Course of elongation factors, rRNA and RPS1 during lysate processing. (A) Cells were lysed using a high- pressure homogenizer (1); the homogenate was clarified by centrifugation (2); a runoff reaction at 37˚C was performed for 80 min (3); the lysate
Fig 3. Relative concentrations of elongation factors and RPS1 during the in vitro translation reaction.
Fig 4. rRNA degradation during the in vitro translation reaction. (A) In vitro translation reaction of eGFP
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