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Differential effects of temperature on reactive oxygen/nitrogen species production in rat pachytene spermatocytes and round spermatids

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Academic year: 2020

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Figure 1 (A) ROS/RNS production rate in round spermatids and pachytene spermatocytes incubated at different temperatures.
Figure 2 Rate of ROS/RNS formation in round spermatids and pachytene spermatocytes in the presence of inhibitors: nitric oxide synthase (LNMA, 10 mM), NADPH (flavin) oxidases (DPI, 20 mM), mitochondrial complex I (rotenone (ROT), 2 mM) and xanthine oxidase
Figure 4 Rate of oxygen consumption (QO 2 ) in (A) round spermatids and (B) pachytene spermatocytes incubated at different temperatures and in the absence (basal) and presence of different effectors of oxidative phosphorylation
Figure 6 shows the changes in the rhodamine 123 (R123) fluorescence ratio (an estimation of  mito-chondrial membrane potential) induced by heat stress (40 8C) in round spermatids and pachytene spermatocyte mitochondria
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