(1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)
(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)
Identificación de complejos organometálicos en
tejidos vegetales mediante espectrometría de
masas
R Rellán-Álvarez, J Abadía and A Álvarez-Fernández
Plant Stress Physiology Group,
Aula Dei Experimental Station, CSIC, Zaragoza
chloroplast
AtCCC1
AtIRT2
AtVIT1
AtSLB AtPIC1vacuole
leaf
xylem
root
Modified from Abadía J, 2006, Pers. Com.
Metal Speciation: A complex
(ed)
story
FRU/FIT/bHLH
PS
AtFRD3
AtYSL2
Cit
OsFRDL1
AtYSL2
AtZIP8
AtFRO3
AtNRAMP3,4
OsYSL2
Fe(II)
AtIRT1
H
+Fe(III)
AtFRO2
Hyphenated techniques available for species-selective analysis of biological materials
Hyphenated techniques available for species-selective analysis of biological materials
Separation
Detection
Identification
Metal Specific
Compound Specific
HPLC
ICP-MS
ESI-TOF-MS
Main problems and bottlenecks
-Sample extraction: should conserve the plant compartment conditions
for example pH values.
Main problems and bottlenecks
Ligand Exchange
pH Dependance
Main problems and bottlenecks
-Sample extraction: should conserve the plant compartment conditions
for example pH values.
-These conditions should also be conserved during separation and detection.
-If this conditions a
-Sensitivity: very low concentration of metals in plant tissues.
-Cost: expensive equipment.
Integrated mass spectrometry in (semi-)metal speciation
HPLC-TOFMS
time (HPLC)
m/z
HPLC-TOFMS Mass spectra at an specific time
m/z
5.9 min
m/z
