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Prostate Cancer Cells Differ in Testosterone Accumulation, Dihydrotestosterone Conversion, and Androgen Receptor Signaling Response to Steroid 5α Reductase Inhibitors

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Fig. 1. IHC for 5 a-reductase-1, -2, and -3 in prostate cancer cells cultured in the presence or absence of1 nM testosterone (T).Note: the pink hue in row 2 was animage acquisition error thatdid not affect analysis and was not affectedby image processing.
Fig. 2. Message level of 5 a-reductase-1, -2, and -3 as determined using qRT-PCR in LNCaP (A), LNCaP-C4-2 (B), LAPC-4 (C), and 22Rv1 (D) cultured either in the presence or absence of1 nM testosterone (T).
Fig. 3. Lackof abilityof LNCaPcells to maintainintracellular testosterone and to convert testosterone to DHT
TABLE I. Intracellular Testosterone (T) and Dihydrotestosterone (DHT) Concentrations (nM) After 24 hr Culture in the Presence of 1 nM T
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