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Ca2+ entry via TRPC1 is essential for cellular differentiation and modulates secretion via the SNARE complex

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Academic year: 2020

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Fig. 1. SOCE is essential for the differentiation of subcutaneous adipocytes. (A,B,F,G) Fura-2 fluorescence traces (F 340 /F 380 , 340 nm:380 nm fluorescence ratio) of transient increase in [Ca 2+ ] i after addition of 1 μM Tg and 1 mM Ca 2+ to Subc-AT SVF
Fig. 2. Differentiated visceral adipocytes also exhibit increase in SOCE. (A,B) Western blots (A) of TRPC1, ORAI1, Stim1, FAPB4 and perilipin, and actin protein expression, protein level quantification normalized to actin provided as bar graph (B), of VAT
Fig. 3. TRPC1 −/− mice have increased adiposity with age. (A) mRNA expression of TRPC1 and TRPC3 from Subc-AT of WT and TRPC1 −/− mice, and western blot of TRPC1 protein expression
Fig. 4. Loss of TRPC1 in subcutaneous adipocytes reduces SOCE and adipogenesis. (A,B,F,G) Fura-2 fluorescence traces of transient increase in [Ca 2+ ] i
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