©RSNA, 2015
10.1148/radiol.2015150800
Appendix E1
Gene Expression Profiling
Training Set
As was previously described, gene expression profiles of the 70 CCRCCs (performed on cDNA
microarrays containing over 40,000 cDNA clones and representing 27,290 unique UniGene
clusters) were accessed from the Gene Expression Omnibus (GSE3538) (1). To filter the
expression data to only those genes that were most variably expressed and well measured, we
used the same filtering criteria as Zhao et al (1); array elements that varied at least two-fold from
the median on at least five microarrays were selected for subsequent analysis (5332 cDNA
elements representing 4825 unique genes). Within these 5332 cDNA elements, transcripts that
were identified as part of the previously determined CCRCC prognostic gene expression
signature were selected (217 transcripts representing 139 genes [Table E1]). Hierarchical
clustering of the expression profiles are shown in Figure E1. Kaplan-Meier survival analysis was
performed between the low and high prognostic patient groups, confirming that the 259
prognostic gene signatures remained a significant predictor of survival in our population (P =
.0031).
Validation Set
Gene expression profiles of the 70 CCRCCs was performed on Illumina Human HT-12 v4
Expression BeadChip arrays (Illumina, San Diego, Calif), which contained 47,231 probes.
Quantile normalization was performed with the lumi R package (2). The expression arrays were
filtered on the basis of the set of SPC transcripts that mapped to the Illumina probes.
References
1. Zhao H, Ljungberg B, Grankvist K, Rasmuson T, Tibshirani R, Brooks JD. Gene expression
profiling predicts survival in conventional renal cell carcinoma. PLoS Med 2006;3(1):e13.
2. Du P, Kibbe WA, Lin SM. lumi: a pipeline for processing Illumina microarray. Bioinformatics
2008;24(13):1547–1548.
Table E1. Annotation of SPC Transcripts
UniGene ID Gene Description
Hs.116651 Myelin protein zero-like 2::myelin protein zero-like 2 || Hs.116651 || 10205 || AA768426 || IMAGE:1324446 || || MPZL2 || || || 100152
Hs.445826 Tubulin tyrosine ligase-like family, member 7::tubulin tyrosine ligase-like family, member 7 || Hs.445826 || 79739 || H29858::H29951 || IMAGE:190256 || || TTLL7 || || || 100456
Hs.148670 Rho-related BTB domain containing 1::Rho-related BTB domain containing 1 || Hs.148670 || 9886 || AA182796::AA187789 || IMAGE:624379 || || RHOBTB1
|| || || 101610
Hs.203691 Discoidin, CUB and LCCL domain containing 2::discoidin, CUB and LCCL domain containing 2 || Hs.203691 || 131566 || H99544::H99543 || IMAGE:263200
|| || DCBLD2 || || || 103198
Hs.83381 Guanine nucleotide binding protein (G protein), gamma 11::guanine nucleotide binding protein (G protein), gamma 11 || Hs.83381 || 2791 || AA999901 || IMAGE:1636447 || || GNG11 || || || 104086
Hs.483993 Palmdelphin::palmdelphin || Hs.483993 || 54873 || AA418728::AA418782 || IMAGE:767823 || || PALMD || || || 104123
Hs.203691 Discoidin, CUB and LCCL domain containing 2::discoidin, CUB and LCCL domain containing 2 || Hs.203691 || 131566 || AI302412 || IMAGE:1901754 || ||
DCBLD2 || || || 104620
Hs.458414 Interferon induced transmembrane protein 1 (9–27)::interferon induced transmembrane protein 1 (9–27) || Hs.458414 || 8519 || AA419251::AA419286 ||
IMAGE:755599 || || IFITM1 || || || 105146
Hs.481342 CDNA clone IMAGE:4791597 || Hs.481342 || || AA142913 || IMAGE:504420 || || || || || 106893
Hs.521240 KIAA1147::KIAA1147 || Hs.521240 || 57189 || AA029331::AA029330 || IMAGE:366763 || || KIAA1147 || || || 107221
Hs.499725 Ankyrin 3, node of Ranvier (ankyrin G)::ankyrin 3, node of Ranvier (ankyrin G)::Transcribed locus || Hs.499725::Hs.592422 || 288 || AA021558::AA021643 || IMAGE:364209 || || ANK3 || || || 107886
Hs.24950 Transcribed locus, strongly similar to NP_003608.1 regulator of G-protein signaling 5 [Homo sapiens] || Hs.24950 || || AA668470 || IMAGE:853809 || || || || ||
109173
Hs.199487 RAS-like, estrogen-regulated, growth inhibitor::RAS-like, estrogen-regulated, growth inhibitor || Hs.199487 || 85004 || AA131239::AA131358 || IMAGE:503581 || || RERG || || || 109293
Hs.7946 Mitochondrial tumor suppressor 1::mitochondrial tumor suppressor 1 || Hs.7946 || 57509 || AA621202 || IMAGE:744395 || || MTUS1 || || || 110080
Hs.124922 Lymphoid-restricted membrane protein::lymphoid-restricted membrane protein || Hs.124922 || 4033 || AA457051::AA456895 || IMAGE:815539 || || LRMP ||
|| || 110391
Hs.463456 Nonmetastatic cells 1, protein (NM23A) expressed in::nonmetastatic cells 1, protein (NM23A) expressed in || Hs.463456 || 4830 || AA496628::AA496512 ||
IMAGE:755750 || || NME1 || || || 110488
Hs.482077 Integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)::integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor) || Hs.482077 || 3673 || AA069027::AA069096 || IMAGE:525246 || || ITGA2 || || || 110574
Hs.50123 Zinc finger protein 189::zinc finger protein 189 || Hs.50123 || 7743 || AA256471::AA256810 || IMAGE:682063 || || ZNF189 || || || 110719
Hs.267659 Vav 3 guanine nucleotide exchange factor::vav 3 guanine nucleotide exchange factor || Hs.267659 || 10451 || W91879::W91952 || IMAGE:415229 || || VAV3
|| || || 112874
Hs.298252 Acyl-CoA synthetase medium-chain family member 2A::acyl-CoA synthetase medium-chain family member 2B || Hs.298252 || 348158 || AA962431 ||
IMAGE:1553550 || || ACSM2B || || || 113404
Hs.237028 Natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C)::natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C) || Hs.237028 || 4883 || AI668687::N92291::AI734266::W24469 || IMAGE:308004 || || NPR3 || || || 113814
Hs.76704 Transcribed locus || Hs.76704 || || T68445::T68510 || IMAGE:83358 || || || || || 11442
Hs.27695 Midline 1 (Opitz/BBB syndrome)::midline 1 (Opitz/BBB syndrome) || Hs.27695 || 4281 || AA598640 || IMAGE:897865 || || MID1 || || || 114753
Hs.436944 Sprouty homolog 1, antagonist of FGF signaling (Drosophila)::sprouty homolog 1, antagonist of FGF signaling (Drosophila)::Transcribed locus || Hs.436944::Hs.706455 || 10252 || AA055440::AA055439 || IMAGE:377468 || || SPRY1 || || || 116132
Hs.483873 Transcribed locus::GM2 ganglioside activator::GM2 ganglioside activator || Hs.469608::Hs.483873 || 2760 || AA983633::AI733175::AI793102 ||
IMAGE:1559530 || || GM2A || || || 116436
Hs.125898 GNAS complex locus::GNAS complex locus::Transcribed locus || Hs.125898::Hs.598953 || 2778 || AA285128::AA284012 || IMAGE:700724 || || GNAS || || || 11669
Hs.94107 Glutathione S-transferase A2::glutathione S-transferase A2 || Hs.94107 || 2939 || T73468::T73535 || IMAGE:82710 || || GSTA2 || || || 11699
Hs.13528 Chromosome 5 open reading frame 23::chromosome 5 open reading frame 23 || Hs.13528 || 79614 || N90806::W19519 || IMAGE:303139 || || C5orf23 || || ||
117332
Hs.94107 Glutathione S-transferase A2::glutathione S-transferase A2::Transcribed locus || Hs.94107::Hs.659570 || 2939 || N59772::N78234 || IMAGE:248545 || ||
GSTA2 || || || 117491
Hs.31439 Serine peptidase inhibitor, Kunitz type, 2::serine peptidase inhibitor, Kunitz type, 2 || Hs.31439 || 10653 || AA031287::AA031401 || IMAGE:470385 || || SPINT2 || || || 11754
Hs.276770 CD52 molecule::CD52 molecule || Hs.276770 || 1043 || AA435559::AA393138 || IMAGE:727988 || || CD52 || || || 117701
Hs.497183 Influenza virus NS1A binding protein::influenza virus NS1A binding protein || Hs.497183 || 10625 || AA487115::AA486796 || IMAGE:841226 || || IVNS1ABP
|| || || 118235
Hs.102267 Lysyl oxidase::lysyl oxidase || Hs.102267 || 4015 || AA452916::AA453085 || IMAGE:789069 || || LOX || || || 11828
Hs.131226 BCL2/adenovirus E1B 19kDa interacting protein 3-like::BCL2/adenovirus E1B 19kDa interacting protein 3-like || Hs.131226 || 665 || AA025112::AA025195 ||
IMAGE:365098 || || BNIP3 L || || || 118405
Hs.237642 Cytochrome P450, family 4, subfamily V, polypeptide 2::kallikrein B, plasma (Fletcher factor) 1 || Hs.237642 || 3818 || AA450041::AA452670 || IMAGE:788445 || || KLKB1 || || || 118599
Hs.125898 GNAS complex locus::GNAS complex locus || Hs.125898 || 2778 || W88587 || IMAGE:417473 || || GNAS || || || 1186
Hs.203691 Discoidin, CUB and LCCL domain containing 2::discoidin, CUB and LCCL domain containing 2 || Hs.203691 || 131566 || AA457544::AA457744 ||
IMAGE:838736 || || DCBLD2 || || || 120159
Hs.271667 EH domain binding protein 1::EH domain binding protein 1 || Hs.271667 || 23301 || AA482066 || IMAGE:756370 || || EHBP1 || || || 120214
Hs.390738 Golgi-localized protein::Golgi-localized protein || Hs.390738 || 55638 || T61269::T61321 || IMAGE:77911 || || GOLSYN || || || 120403
Hs.379858 Dimethylarginine dimethylaminohydrolase 1::dimethylarginine dimethylaminohydrolase 1 || Hs.379858 || 23576 || AA478470::AA451960 || IMAGE:786602 || || DDAH1 || || || 120649
Hs.102267 Lysyl oxidase::lysyl oxidase::Transcribed locus || Hs.102267::Hs.655204 || 4015 || H99075::N26939 || IMAGE:262060 || || LOX || || || 120899
Hs.516578 Tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)::tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) || Hs.516578 || 7035 || AI985214 || IMAGE:2495781 || || TFPI || || || 121601
Hs.302772 SH3 domain binding glutamic acid-rich protein like 2::SH3 domain binding glutamic acid-rich protein like 2 || Hs.302772 || 83699 || W69271::W69558 ||
IMAGE:343760 || || SH3BGRL2 || || || 122318
Hs.485557 Glutathione S-transferase A4::glutathione S-transferase A4 || Hs.485557 || 2941 || N47484::N47483 || IMAGE:280769 || || GSTA4 || || || 122984
Hs.534322 Major histocompatibility complex, class II, DR beta 3::major histocompatibility complex, class II, DR beta 1 || Hs.534322 || 3123 || H52245::H52342 ||
IMAGE:235903 || || HLA-DRB1 || || || 123156
Hs.82002 Endothelin receptor type B::endothelin receptor type B || Hs.82002 || 1910 || N29914::N42964 || IMAGE:271050 || || EDNRB || || || 123435
Hs.461086 Cadherin 1, type 1, E-cadherin (epithelial)::cadherin 1, type 1, E-cadherin (epithelial) || Hs.461086 || 999 || W86859 || IMAGE:416386 || || CDH1 || || || 123594
Hs.8364 Pyruvate dehydrogenase kinase, isozyme 4::pyruvate dehydrogenase kinase, isozyme 4 || Hs.8364 || 5166 || AA169469 || IMAGE:594120 || || PDK4 || || ||
123610
Hs.135507 Egl nine homolog 3 (C. elegans)::egl nine homolog 3 (C. elegans) || Hs.135507 || 112399 || AA991861 || IMAGE:1610464 || || EGLN3 || || || 124347
Hs.133421 Leukemia inhibitory factor receptor alpha::leukemia inhibitory factor receptor alpha::Transcribed locus || Hs.133421::Hs.594542 || 3977 || H10192::H10240 ||
Hs.491336 Elongation protein 3 homolog (S. cerevisiae)::elongation protein 3 homolog (S. cerevisiae) || Hs.491336 || 55140 || AA482031::AA417592 || IMAGE:746072
|| || ELP3 || || || 125196
Hs.458414 Interferon induced transmembrane protein 1 (9–27)::interferon induced transmembrane protein 1 (9–27) || Hs.458414 || 8519 || AA055586 || IMAGE:510383 || || IFITM1 || || || 12538
Hs.293917 Poliovirus receptor-related 3::poliovirus receptor-related 3 || Hs.293917 || 25945 || AA905273 || IMAGE:1506126 || || PVRL3 || || || 126238
Hs.133421 Leukemia inhibitory factor receptor alpha::leukemia inhibitory factor receptor alpha::serine hydroxymethyltransferase 2 (mitochondrial)::Serine
hydroxymethyltransferase 2 (mitochondrial) || Hs.133421::Hs.714300 || 3977::6472 || N67017::W04138 || IMAGE:295889 || || LIFR::SHMT2 || || || 127915
Hs.308628 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4::ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 || Hs.308628 || 7903 ||
AA994501 || IMAGE:1628880 || || ST8SIA4 || || || 128136
Hs.293970 Aldehyde dehydrogenase 6 family, member A1::aldehyde dehydrogenase 6 family, member A1 || Hs.293970 || 4329 || AA196287::AA196160 || IMAGE:627687 || || ALDH6A1 || || || 129079
Hs.401954 Transmembrane and tetratricopeptide repeat containing 1::transmembrane and tetratricopeptide repeat containing 1 || Hs.401954 || 83857 || T60061 ||
IMAGE:81316 || || TMTC1 || || || 129741
Hs.5025 Nebulette::nebulette || Hs.5025 || 10529 || N77806::W16723 || IMAGE:302188 || || NEBL || || || 130866
Hs.368592 Sortilin-related receptor, L (DLR class) A repeats-containing::sortilin-related receptor, L (DLR class) A repeats-containing || Hs.368592 || 6653 ||
T51538::T51689 || IMAGE:72391 || || SORL1 || || || 131034
Hs.133397 Integrin, alpha 6::integrin, alpha 6 || Hs.133397 || 3655 || H07142::H06635 || IMAGE:44814 || || ITGA6 || || || 131452
Hs.522818 L1 cell adhesion molecule::L1 cell adhesion molecule || Hs.522818 || 3897 || AI671234 || IMAGE:2305693 || || L1CAM || || || 131458
Hs.212838 Alpha-2-macroglobulin::alpha-2-macroglobulin || Hs.212838 || 2 || 2::AA775447 || IMAGE:878182 || || A2M || || || 132109
Hs.461086 Cadherin 1, type 1, E-cadherin (epithelial)::cadherin 1, type 1, E-cadherin (epithelial) || Hs.461086 || 999 || T62868::T62718 || IMAGE:79598 || || CDH1 || || ||
132240
Hs.131226 BCL2/adenovirus E1B 19kDa interacting protein 3-like::BCL2/adenovirus E1B 19kDa interacting protein 3-like || Hs.131226 || 665 || AA465697 ||
IMAGE:814899 || || BNIP3 L || || || 133103
Hs.203691 Discoidin, CUB and LCCL domain containing 2::discoidin, CUB and LCCL domain containing 2 || Hs.203691 || 131566 || N21309::N31244 || IMAGE:265494 || || DCBLD2 || || || 135320
Hs.491336 Elongation protein 3 homolog (S. cerevisiae)::elongation protein 3 homolog (S. cerevisiae) || Hs.491336 || 55140 || AA454745 || IMAGE:809788 || || ELP3 ||
|| || 135562
Hs.109225 Vascular cell adhesion molecule 1::vascular cell adhesion molecule 1 || Hs.109225 || 7412 || H16591::H16637 || IMAGE:49164 || || VCAM1 || || || 135985
Hs.531642 RAB11 family interacting protein 3 (class II)::RAB11 family interacting protein 3 (class II) || Hs.531642 || 9727 || R38361::T77413 || IMAGE:23794 || ||
RAB11FIP3 || || || 137238
Hs.438236 Actin binding LIM protein 1::actin binding LIM protein 1 || Hs.438236 || 3983 || AA406601 || IMAGE:753692 || || ABLIM1 || || || 137804
Hs.62661 Guanylate binding protein 3::guanylate binding protein 1, interferon-inducible, 67kDa || Hs.62661 || 2633 || AA486850::AA486849 || IMAGE:841008 || || GBP1 || || || 138209
Hs.483067 chromosome 5 open reading frame 13::Chromosome 5 open reading frame 13 || Hs.483067 || 9315 || H60560::H60254 || IMAGE:207826 || || C5orf13 || || ||
138506
Hs.150749 B-cell CLL/lymphoma 2::B-cell CLL/lymphoma 2 || Hs.150749 || 596 || W63749::W61100 || IMAGE:342181 || || BCL2 || || || 13936
Hs.31439 Serine peptidase inhibitor, Kunitz type, 2::serine peptidase inhibitor, Kunitz type, 2 || Hs.31439 || 10653 || AA458849::AA459039 || IMAGE:814378 || ||
SPINT2 || || || 139584
Hs.200136 myosin VB::Acetyl-Coenzyme A acyltransferase 2 || Hs.200136 || 4645 || AA219282::AA219466 || IMAGE:629944 || || MYO5B || || || 139613
Hs.473152 Transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma)::transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma)::Transcribed locus || Hs.473152::Hs.713797 || 7022 || AA394236::AA399334 || IMAGE:725680 || || TFAP2C || || || 14224
Hs.369042 MOCO sulphurase C-terminal domain containing 2::MOCO sulphurase C-terminal domain containing 2::Transcribed locus || Hs.369042::Hs.707361 || 54996 || T63490::T63565 || IMAGE:81449 || || MOSC2 || || || 14870
Hs.490203 Caldesmon 1::caldesmon 1 || Hs.490203 || 800 || AA447737::AA453669 || IMAGE:813627 || || CALD1 || || || 15173
Hs.201034 Netrin 4::netrin 4 || Hs.201034 || 59277 || R76614::R76613 || IMAGE:143661 || || NTN4 || || || 1541
Hs.473087 CTP synthase::CTP synthase || Hs.473087 || 1503 || W44416::W45678 || IMAGE:323644 || || CTPS || || || 15929
Hs.8379 Transcribed locus || Hs.8379 || || T41071::T40203 || IMAGE:62093 || || || || || 16288
Hs.497148 Ral guanine nucleotide dissociation stimulator-like 1::ral guanine nucleotide dissociation stimulator-like 1::actin related protein 2/3 complex, subunit 5,
16kDa::Actin related protein 2/3 complex, subunit 5, 16kDa || Hs.497148::Hs.703792 || 23179::10092 || AA169173::AA169293 || IMAGE:610006 || || RGL1::ARPC5 || || || 16338
Hs.126825 Family with sequence similarity 131, member C::family with sequence similarity 131, member C || Hs.126825 || 348487 || AA931428 || IMAGE:1555233 || || FAM131C || || || 16866
Hs.516578 Tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)::tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) ||
Hs.516578 || 7035 || H91294::H91389 || IMAGE:241038 || || TFPI || || || 17883
Hs.486489 Ectonucleotide pyrophosphatase/phosphodiesterase 3::ectonucleotide pyrophosphatase/phosphodiesterase 3 || Hs.486489 || 5169 || AA678335 || IMAGE:430968 || || ENPP3 || || || 1804
Hs.249184 POU class 5 homeobox 1::POU class 5 homeobox 1 || Hs.249184 || 5460 || AA996055 || IMAGE:1607129 || || POU5F1 || || || 18062
Hs.485557 Glutathione S-transferase A4::glutathione S-transferase A4 || Hs.485557 || 2941 || AA152347::AA152346 || IMAGE:504791 || || GSTA4 || || || 18274
Hs.444329 Glypican 6::glypican 6 || Hs.444329 || 10082 || AA456147::AA459880 || IMAGE:796388 || || GPC6 || || || 18465
Hs.129944 Endothelial cell-specific molecule 1::endothelial cell-specific molecule 1 || Hs.129944 || 11082 || W46577::W46667 || IMAGE:324122 || || ESM1 || || || 1968
Hs.283749 Angiogenin, ribonuclease, RNase A family, 5::angiogenin, ribonuclease, RNase A family, 5 || Hs.283749 || 283 || AA682399 || IMAGE:461761 || || ANG || || || 19736
Hs.293970 Aldehyde dehydrogenase 6 family, member A1::aldehyde dehydrogenase 6 family, member A1 || Hs.293970 || 4329 || N62179::N77107 || IMAGE:289818 ||
Hs.499725 Ankyrin 3, node of Ranvier (ankyrin G)::ankyrin 3, node of Ranvier (ankyrin G) || Hs.499725 || 288 || AA677185 || IMAGE:454672 || || ANK3 || || || 20364
Hs.497183 Influenza virus NS1A binding protein::influenza virus NS1A binding protein || Hs.497183 || 10625 || AI968568 || IMAGE:2514707 || || IVNS1ABP || || || 20495
Hs.97270 Family with sequence similarity 13, member A1::family with sequence similarity 13, member A1 || Hs.97270 || 10144 || N51424 || IMAGE:283301 || ||
FAM13A1 || || || 20808
Hs.124951 Family with sequence similarity 84, member B::family with sequence similarity 84, member B || Hs.124951 || 157638 || AI022800 || IMAGE:1650748 || || FAM84B || || || 21010
Hs.154299 Coagulation factor II (thrombin) receptor-like 1::coagulation factor II (thrombin) receptor-like 1::Transcribed locus || Hs.154299::Hs.595395 || 2150 ||
AA454652::AA456265 || IMAGE:811899 || || F2RL1 || || || 21660
Hs.293970 Aldehyde dehydrogenase 6 family, member A1::aldehyde dehydrogenase 6 family, member A1 || Hs.293970 || 4329 || AI859300 || IMAGE:2435471 || ||
ALDH6A1 || || || 21717
Hs.368592 Sortilin-related receptor, L (DLR class) A repeats-containing::sortilin-related receptor, L (DLR class) A repeats-containing || Hs.368592 || 6653 || AI817699 || IMAGE:2413337 || || SORL1 || || || 22389
Hs.479384 KIAA0746 protein::KIAA0746 protein || Hs.479384 || 23231 || AA456569 || IMAGE:809374 || || KIAA0746 || || || 22766
Hs.26039 CDNA FLJ13937 fis, clone Y79AA1000805 || Hs.26039 || || R53446::R53445 || IMAGE:40038 || || || || || 2287
Hs.458414 Interferon induced transmembrane protein 1 (9–27)::interferon induced transmembrane protein 1 (9–27) || Hs.458414 || 8519 || AA058323::AA058453 || IMAGE:509641 || || IFITM1 || || || 22883
Hs.285887 UDP glucuronosyltransferase 2 family, polypeptide B4::UDP glucuronosyltransferase 2 family, polypeptide B4 || Hs.285887 || 7363 || N53031::N73214 ||
IMAGE:246430 || || UGT2B4 || || || 23045
Hs.3281 Neuronal pentraxin II::neuronal pentraxin II || Hs.3281 || 4885 || AA683041 || IMAGE:971372 || || NPTX2 || || || 23516
Hs.279245 Transforming, acidic coiled-coil containing protein 1::transforming, acidic coiled-coil containing protein 1 || Hs.279245 || 6867 || H47327::H47413 ||
Hs.154299 Coagulation factor II (thrombin) receptor-like 1::coagulation factor II (thrombin) receptor-like 1 || Hs.154299 || 2150 || AI023006 || IMAGE:1650942 || ||
F2RL1 || || || 23964
Hs.148907 Solute carrier family 5 (sodium/glucose cotransporter), member 12::solute carrier family 5 (sodium/glucose cotransporter), member 12 || Hs.148907 || 159963 || AA994816::AI733508 || IMAGE:1635099 || || SLC5A12 || || || 25073
Hs.66 Interleukin 1 receptor-like 1::interleukin 1 receptor-like 1 || Hs.66 || 9173 || AA128153::AA125917 || IMAGE:501994 || || IL1RL1 || || || 25183
Hs.237028 Natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C)::natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic
peptide receptor C) || Hs.237028 || 4883 || AI222701 || IMAGE:1762111 || || NPR3 || || || 2580
Hs.133421 Leukemia inhibitory factor receptor alpha::leukemia inhibitory factor receptor alpha || Hs.133421 || 3977 || AA131466::AA131648 || IMAGE:503741 || || LIFR
|| || || 26140
Hs.258212 Transmembrane protein 159::transmembrane protein 159 || Hs.258212 || 57146 || H09934::H09933 || IMAGE:46355 || || TMEM159 || || || 26253
Hs.351798 TBC1 domain family, member 8B (with GRAM domain)::TBC1 domain family, member 8B (with GRAM domain) || Hs.351798 || 54885 || AA287032::AA284464 || IMAGE:701579 || || TBC1D8B || || || 26552
Hs.199487 RAS-like, estrogen-regulated, growth inhibitor::RAS-like, estrogen-regulated, growth inhibitor || Hs.199487 || 85004 || AA037415 || IMAGE:484600 || ||
RERG || || || 26628
Hs.170009 Transforming growth factor, alpha::transforming growth factor, alpha || Hs.170009 || 7039 || AA037107 || IMAGE:325822 || || TGFA || || || 28440
Hs.126137 Acyl-CoA thioesterase 7::acyl-CoA thioesterase 7::Transcribed locus || Hs.126137::Hs.705753 || 11332 || AA035455::AA035123 || IMAGE:471799 || ||
ACOT7 || || || 28782
Hs.465744 insulin receptor::Insulin receptor || Hs.465744 || 3643 || T47312::T47311 || IMAGE:70749 || || INSR || || || 29726
Hs.490203 Caldesmon 1::caldesmon 1::Transcribed locus || Hs.490203::Hs.596580 || 800 || AA426421::AA437143 || IMAGE:758088 || || CALD1 || || || 29980
Hs.434957 Homo sapiens, clone IMAGE:3618365, mRNA || Hs.434957 || || H19307::H19013 || IMAGE:51015 || || || || || 30057
Hs.97270 Family with sequence similarity 13, member A1::family with sequence similarity 13, member A1 || Hs.97270 || 10144 || AA932564 || IMAGE:1570311 || ||
Hs.117067 Transcribed locus || Hs.117067 || || AA677561 || IMAGE:460435 || || || || || 30602
Hs.482077 Integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)::integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor) || Hs.482077 || 3673 ||
AA993294 || IMAGE:1623955 || || ITGA2 || || || 30654
Hs.499886 Aldehyde dehydrogenase 3 family, member A2::aldehyde dehydrogenase 3 family, member A2 || Hs.499886 || 224 || AA418697::AA418758 || IMAGE:767804 || || ALDH3A2 || || || 30672
Hs.406238 Aldehyde oxidase 1::aldehyde oxidase 1 || Hs.406238 || 316 || AI343711 || IMAGE:1917741 || || AOX1 || || || 31430
Hs.279245 Transforming, acidic coiled-coil containing protein 1::transforming, acidic coiled-coil containing protein 1 || Hs.279245 || 6867 || AA664006 || IMAGE:855389
|| || TACC1 || || || 31706
Hs.490203 Caldesmon 1::caldesmon 1 || Hs.490203 || 800 || H52087::H51958 || IMAGE:197450 || || CALD1 || || || 3788
Hs.156967 Sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A::sema domain, transmembrane domain (TM), and cytoplasmic
domain, (semaphorin) 6A || Hs.156967 || 57556 || AA236561::AA234621 || IMAGE:687579 || || SEMA6A || || || 3980
Hs.195060 Chromosome 11 open reading frame 57::chromosome 11 open reading frame 57::Transcribed locus, strongly similar to NP_001076439.1 hypothetical protein LOC55216 isoform b [Homo sapiens] || Hs.195060::Hs.644677 || 55216 || W04502::W31402 || IMAGE:320146 || || C11orf57 || || || 4250
Hs.308628 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4::ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 || Hs.308628 || 7903 || AA921677 || IMAGE:1536864 || || ST8SIA4 || || || 4779
Hs.463613 Yippee-like 2 (Drosophila)::yippee-like 2 (Drosophila) || Hs.463613 || 388403 || AA903218 || IMAGE:1517749 || || YPEL2 || || || 55474
Hs.7946 Mitochondrial tumor suppressor 1::mitochondrial tumor suppressor 1 || Hs.7946 || 57509 || AA496896::AA496857 || IMAGE:897603 || || MTUS1 || || || 55633
Hs.531642 RAB11 family interacting protein 3 (class II)::RAB11 family interacting protein 3 (class II) || Hs.531642 || 9727 || R44896::R24299 || IMAGE:33977 || ||
RAB11FIP3 || || || 55744
Hs.4104 Chromosome 14 open reading frame 129::chromosome 14 open reading frame 129::Transcribed locus || Hs.592297::Hs.4104 || 51527 ||
Hs.401954 Transmembrane and tetratricopeptide repeat containing 1::transmembrane and tetratricopeptide repeat containing 1 || Hs.401954 || 83857 ||
AA447480::AA447479 || IMAGE:784276 || || TMTC1 || || || 57065
Hs.904 Amylo-1, 6-glucosidase, glucanotransferase (glycogen debranching enzyme, glycogen storage disease type III)::amylo-1, 6-glucosidase, 4-alpha-glucanotransferase || Hs.904 || 178 || AA668425 || IMAGE:853687 || || AGL || || || 59181
Hs.25590 Stanniocalcin 1::stanniocalcin 1 || Hs.25590 || 6781 || AA085319::AA085318 || IMAGE:547247 || || STC1 || || || 60080
Hs.276770 CD52 molecule::CD52 molecule || Hs.276770 || 1043 || AI826477 || IMAGE:2417330 || || CD52 || || || 62436
Hs.438236 Actin binding LIM protein 1::actin binding LIM protein 1 || Hs.438236 || 3983 || T50244::AI821042 || IMAGE:77325 || || ABLIM1 || || || 62928
Hs.97270 Family with sequence similarity 13, member A1::family with sequence similarity 13, member A1 || Hs.97270 || 10144 || AA024827::AA024932 || IMAGE:364992 || || FAM13A1 || || || 63091
Hs.82002 Endothelin receptor type B::endothelin receptor type B || Hs.82002 || 1910 || H28710::H28840 || IMAGE:49665 || || EDNRB || || || 63244
Hs.481918 Solute carrier family 1 (glial high affinity glutamate transporter), member 3::solute carrier family 1 (glial high affinity glutamate transporter), member 3 ||
Hs.481918 || 6507 || AA453742::AA453823 || IMAGE:813678 || || SLC1A3 || || || 63347
Hs.463456 Nonmetastatic cells 1, protein (NM23A) expressed in::nonmetastatic cells 1, protein (NM23A) expressed in || Hs.463456 || 4830 || AA644092 ||
IMAGE:845363 || || NME1 || || || 64259
Hs.468410 Endothelial PAS domain protein 1::endothelial PAS domain protein 1 || Hs.468410 || 2034 || AA680300 || IMAGE:869187 || || EPAS1 || || || 64646
Hs.125898 GNAS complex locus::GNAS complex locus || Hs.125898 || 2778 || AA035620::AA062962 || IMAGE:359933 || || GNAS || || || 6533
Hs.20575 Growth arrest-specific 2 like 3::growth arrest-specific 2 like 3 || Hs.20575 || 283431 || AI292036 || IMAGE:1894782 || || GAS2 L3 || || || 65785
Hs.229641 SUB1 homolog (S. cerevisiae)::SUB1 homolog (S. cerevisiae) || Hs.229641 || 10923 || AA456973::AA457082 || IMAGE:838628 || || SUB1 || || || 65825
Hs.379858 Dimethylarginine dimethylaminohydrolase 1::dimethylarginine dimethylaminohydrolase 1 || Hs.379858 || 23576 || N24042::N35863 || IMAGE:269300 || || DDAH1 || || || 66013
Hs.190477 Zinc finger CCCH-type containing 6::zinc finger CCCH-type containing 6 || Hs.190477 || 376940 || AA948719::AI733399::AI791811 || IMAGE:1589343 || ||
ZC3H6 || || || 66267
Hs.534322 Major histocompatibility complex, class II, DR beta 3::major histocompatibility complex, class II, DR beta 1 || Hs.534322 || 3123 || AA630549 || IMAGE:855258 || || HLA-DRB1 || || || 66284
Hs.497575 SLIT-ROBO Rho GTPase activating protein 2::SLIT-ROBO Rho GTPase activating protein 2 || Hs.497575 || 23380 || N31484 || IMAGE:272155 || ||
SRGAP2 || || || 66393
Hs.302772 SH3 domain binding glutamic acid-rich protein like 2::SH3 domain binding glutamic acid-rich protein like 2 || Hs.302772 || 83699 || H19327::H19036 ||
IMAGE:50926 || || SH3BGRL2 || || || 66497
Hs.266175 Phosphoprotein associated with glycosphingolipid microdomains 1::phosphoprotein associated with glycosphingolipid microdomains 1 || Hs.266175 || 55824 || H09567::H08999 || IMAGE:45934 || || PAG1 || || || 67540
Hs.279245 Transforming, acidic coiled-coil containing protein 1::transforming, acidic coiled-coil containing protein 1 || Hs.279245 || 6867 || AA456316::AA456713 ||
IMAGE:813188 || || TACC1 || || || 68481
Hs.496969 N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase)::N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) || Hs.496969 ||
80896 || W78156::W79930 || IMAGE:346861 || || NPL || || || 68680
Hs.498494 Proprotein convertase subtilisin/kexin type 6::proprotein convertase subtilisin/kexin type 6::Proprotein convertase subtilisin/kexin type 6 || Hs.498494::Hs.665989 || 5046 || W85807::W85806 || IMAGE:415962 || || PCSK6 || || || 68811
Hs.368592 Sortilin-related receptor, L (DLR class) A repeats-containing::sortilin-related receptor, L (DLR class) A repeats-containing || Hs.368592 || 6653 || AA424516
|| IMAGE:767069 || || SORL1 || || || 69282
Hs.438236 Actin binding LIM protein 1::actin binding LIM protein 1 || Hs.438236 || 3983 || AI982577 || IMAGE:2516036 || || ABLIM1 || || || 6947
Hs.474914 Small G protein signaling modulator 3::small G protein signaling modulator 3 || Hs.474914 || 27352 || AA971563 || IMAGE:1583501 || || SGSM3 || || || 70273
Hs.37982 Transcribed locus || Hs.37982 || || T61428 || IMAGE:79848 || || || || || 70548
Hs.129614 Transmembrane protein 27::transmembrane protein 27 || Hs.129614 || 57393 || AA999850::AI792241 || IMAGE:1636365 || || TMEM27 || || || 71991
Hs.133397 Integrin, alpha 6::integrin, alpha 6 || Hs.133397 || 3655 || T54663::T54750 || IMAGE:73784 || || ITGA6 || || || 72419
Hs.129614 Transmembrane protein 27::transmembrane protein 27 || Hs.129614 || 57393 || AI253234::AI793193::AI793025 || IMAGE:2029266 || || TMEM27 || || ||
72509
Hs.167451 KIAA0999 protein::KIAA0999 protein || Hs.167451 || 23387 || AA446193::AA446192 || IMAGE:781151 || || KIAA0999 || || || 73946
Hs.271667 EH domain binding protein 1::EH domain binding protein 1 || Hs.271667 || 23301 || AA456284::AA456682 || IMAGE:813144 || || EHBP1 || || || 74227
Hs.125898 GNAS complex locus::GNAS complex locus || Hs.125898 || 2778 || AI268751 || IMAGE:1911721 || || GNAS || || || 7441
Hs.497148 Ral guanine nucleotide dissociation stimulator-like 1::ral guanine nucleotide dissociation stimulator-like 1 || Hs.497148 || 23179 || T98762::T98761 ||
IMAGE:122229 || || RGL1 || || || 75486
Hs.238990 Cyclin-dependent kinase inhibitor 1B (p27, Kip1)::cyclin-dependent kinase inhibitor 1B (p27, Kip1) || Hs.238990 || 1027 || AA192725::AA192724 || IMAGE:627509 || || CDKN1B || || || 76143
Hs.196952 Hepatic leukemia factor::hepatic leukemia factor || Hs.196952 || 3131 || N70235::W00959 || IMAGE:296587 || || HLF || || || 7753
Hs.368592 Sortilin-related receptor, L (DLR class) A repeats-containing::sortilin-related receptor, L (DLR class) A repeats-containing || Hs.368592 || 6653 ||
N50656::N50751 || IMAGE:280786 || || SORL1 || || || 78113
Hs.269004 Solute carrier family 36 (proton/amino acid symporter), member 1::solute carrier family 36 (proton/amino acid symporter), member 1 || Hs.269004 || 206358
|| R00480::R00479 || IMAGE:123331 || || SLC36A1 || || || 78964
Hs.25590 Stanniocalcin 1::stanniocalcin 1 || Hs.25590 || 6781 || AA126561::AA126659 || IMAGE:565075 || || STC1 || || || 7940
Hs.267659 Vav 3 guanine nucleotide exchange factor::vav 3 guanine nucleotide exchange factor || Hs.267659 || 10451 || H10045::H10098 || IMAGE:46827 || || VAV3 || || || 79496
Hs.109225 Vascular cell adhesion molecule 1::vascular cell adhesion molecule 1 || Hs.109225 || 7412 || H07072::H07071 || IMAGE:44477 || || VCAM1 || || || 79543
Hs.437322 Tumor necrosis factor, alpha-induced protein 6::tumor necrosis factor, alpha-induced protein 6 || Hs.437322 || 7130 || W92764::W93163 || IMAGE:357031 ||
|| TNFAIP6 || || || 79961
Hs.283749 Angiogenin, ribonuclease, RNase A family, 5::angiogenin, ribonuclease, RNase A family, 5 || Hs.283749 || 283 || T60163::T60223 || IMAGE:81417 || || ANG || || || 80324
Hs.465413 Cytochrome b5 type A (microsomal)::cytochrome b5 type A (microsomal) || Hs.465413 || 1528 || R91950::R92281 || IMAGE:196189 || || CYB5A || || || 80535
Hs.102267 Lysyl oxidase::lysyl oxidase || Hs.102267 || 4015 || W60414::W60413 || IMAGE:341680 || || LOX || || || 81223
Hs.537002 CDNA clone IMAGE:4811759 || Hs.537002 || || AA235370 || IMAGE:687667 || || || || || 81451
Hs.200136 myosin VB::Acetyl-Coenzyme A acyltransferase 2 || Hs.200136 || 4645 || H07926::H08029 || IMAGE:45376 || || MYO5B || || || 81599
Hs.461086 Cadherin 1, type 1, E-cadherin (epithelial)::cadherin 1, type 1, E-cadherin (epithelial) || Hs.461086 || 999 || H97778 || IMAGE:251019 || || CDH1 || || || 8196
Hs.164060 Growth factor receptor-bound protein 10::growth factor receptor-bound protein 10 || Hs.164060 || 2887 || AA136336 || IMAGE:565379 || || GRB10 || || || 82148
Hs.496969 N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase)::N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) || Hs.496969 ||
80896 || AA233620::AA233418 || IMAGE:666159 || || NPL || || || 8293
Hs.465744 insulin receptor::Insulin receptor || Hs.465744 || 3643 || AA001614::AA001106 || IMAGE:427812 || || INSR || || || 83537
Hs.190977 Ectonucleotide pyrophosphatase/phosphodiesterase 2 (autotaxin)::ectonucleotide pyrophosphatase/phosphodiesterase 2 (autotaxin) || Hs.190977 || 5168 ||
AA476508::AA449181 || IMAGE:785351 || || ENPP2 || || || 85382
Hs.535297 AT rich interactive domain 5B (MRF1-like)::AT rich interactive domain 5B (MRF1-like) || Hs.535297 || 84159 || AA135616::AA135695 || IMAGE:501540 || || ARID5B || || || 85766
Hs.16426 podocalyxin-like::Podocalyxin-like::Transcribed locus || Hs.16426::Hs.645175 || 5420 || N64508::N80294 || IMAGE:290378 || || PODXL || || || 86245
Hs.534373 vesicle-associated membrane protein 5 (myobrevin)::Vesicle-associated membrane protein 5 (myobrevin) || Hs.534373 || 10791 || AA521036 ||
Hs.174768 Transcribed locus || Hs.174768 || || AA451891 || IMAGE:786672 || || || || || 8740
Hs.8944 Procollagen C-endopeptidase enhancer 2::procollagen C-endopeptidase enhancer 2 || Hs.8944 || 26577 || AA115742::AA133448 || IMAGE:490647 || ||
PCOLCE2 || || || 87426
Hs.20575 Growth arrest-specific 2 like 3::growth arrest-specific 2 like 3 || Hs.20575 || 283431 || R84407 || IMAGE:194656 || || GAS2 L3 || || || 885
Hs.192586 Carboxymethylenebutenolidase homolog (Pseudomonas)::carboxymethylenebutenolidase homolog (Pseudomonas) || Hs.192586 || 134147 || AA111975::AA083693 || IMAGE:530197 || || CMBL || || || 88912
Hs.527078 Peroxisome proliferator-activated receptor gamma, coactivator 1 alpha::peroxisome proliferator-activated receptor gamma, coactivator 1 alpha || Hs.527078
|| 10891 || N89673::W21597 || IMAGE:307050 || || PPARGC1A || || || 8967
Hs.282795 CDNA FLJ26512 fis, clone KDN07513 || Hs.282795 || || N76019::W04370 || IMAGE:295323 || || || || || 89690
Hs.490203 Caldesmon 1::caldesmon 1 || Hs.490203 || 800 || H48508::H48677 || IMAGE:207107 || || CALD1 || || || 90633
Hs.374990 CD34 molecule::CD34 molecule || Hs.374990 || 947 || H72113::H72215 || IMAGE:213635 || || CD34 || || || 91109
Hs.500333 Chromosome 10 open reading frame 58::chromosome 10 open reading frame 58 || Hs.500333 || 84293 || AI339215 || IMAGE:1946834 || || C10orf58 || || ||
91701
Hs.120949 CD36 molecule (thrombospondin receptor)::CD36 molecule (thrombospondin receptor) || Hs.120949 || 948 || N39161::N45238 || IMAGE:243816 || || CD36 ||
|| || 91726
Hs.266175 Phosphoprotein associated with glycosphingolipid microdomains 1::phosphoprotein associated with glycosphingolipid microdomains 1 || Hs.266175 || 55824 || AA938646 || IMAGE:1574067 || || PAG1 || || || 92463
Hs.249184 POU class 5 homeobox 1::POU class 5 homeobox 1 || Hs.249184 || 5460 || AI798436 || IMAGE:2220269 || || POU5F1 || || || 92579
Hs.23388 DEAD (Asp-Glu-Ala-Asp) box polypeptide 47::DEAD (Asp-Glu-Ala-Asp) box polypeptide 47 || Hs.23388 || 51202 || AA432292::AA431105 || IMAGE:781468
|| || DDX47 || || || 92603
Hs.232054 Solute carrier family 47, member 1::solute carrier family 47, member 1 || Hs.232054 || 55244 || W85883::W85967 || IMAGE:416096 || || SLC47A1 || || ||
93700
Hs.308628 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4::ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 || Hs.308628 || 7903 || AA913822 || IMAGE:1525823 || || ST8SIA4 || || || 93902
Hs.8364 Pyruvate dehydrogenase kinase, isozyme 4::pyruvate dehydrogenase kinase, isozyme 4 || Hs.8364 || 5166 || T61792 || IMAGE:78946 || || PDK4 || || || 943
Hs.133397 Integrin, alpha 6::integrin, alpha 6 || Hs.133397 || 3655 || R43483::R17993 || IMAGE:32493 || || ITGA6 || || || 9433
Hs.482390 Transforming growth factor, beta receptor III::transforming growth factor, beta receptor III || Hs.482390 || 7049 || N26658::N39809 || IMAGE:269269 || ||
TGFBR3 || || || 9507
Hs.116651 Myelin protein zero-like 2::myelin protein zero-like 2 || Hs.116651 || 10205 || AA668897 || IMAGE:853998 || || MPZL2 || || || 95950
Hs.468410 Endothelial PAS domain protein 1::endothelial PAS domain protein 1 || Hs.468410 || 2034 || R32440::R24882 || IMAGE:131979 || || EPAS1 || || || 96487
Hs.351279 Major histocompatibility complex, class II, DM alpha::major histocompatibility complex, class II, DM alpha || Hs.351279 || 3108 || H42728::H42679 ||
IMAGE:183337 || || HLA-DMA || || || 97058
Hs.535297 AT rich interactive domain 5B (MRF1-like)::AT rich interactive domain 5B (MRF1-like) || Hs.535297 || 84159 || AA173611::AA173610 || IMAGE:595637 || ||
ARID5B || || || 97438
Hs.531776 Lectin, galactoside-binding, soluble, 2::lectin, galactoside-binding, soluble, 2 || Hs.531776 || 3957 || AA872397 || IMAGE:1472743 || || LGALS2 || || || 98154
Hs.497148 Ral guanine nucleotide dissociation stimulator-like 1::ral guanine nucleotide dissociation stimulator-like 1 || Hs.497148 || 23179 || AA683557 || IMAGE:505864 || || RGL1 || || || 98816
Hs.460217 IQ motif containing K::IQ motif containing K::Transcribed locus || Hs.460217::Hs.656664 || 124152 || R43873::R19521 || IMAGE:33603 || || IQCK || || ||
98973
Hs.293917 Poliovirus receptor-related 3::poliovirus receptor-related 3::Transcribed locus || Hs.293917::Hs.655356 || 25945 || T40950::T40020 || IMAGE:61502 || ||
Hs.521240 KIAA1147::KIAA1147::Transcribed locus, strongly similar to NP_082328.2 synaptoporin [Mus musculus] || Hs.521240::Hs.659148 || 57189 ||
H98619::N25140 || IMAGE:261492 || || KIAA1147 || || || 9982
Hs.237642 Cytochrome P450, family 4, subfamily V, polypeptide 2::kallikrein B, plasma (Fletcher factor) 1 || Hs.237642 || 3818 || W90457::W90456 || IMAGE:417884 || || KLKB1 || || || 99822
Table E2. Definitions of Imaging Traits
Trait No. Trait Name Trait Description Stage Dependency Trait Values
1 Tumor size Maximal cross sectional diameter of the tumor (cm) Dependent Quantitative*
2 Renal capsule involvement Renal capsule involvement: does the tumor involve the renal capsule (yes/no) Dependent Binary
3 Gerota’s fascia involvement Gerota fascia involvement: does the tumor invade or involve Gerota’s fascia (yes or
no)
Dependent Binary
4 Infiltration of peritumoral fat Tumoral Infiltration of peritumoral fat (present or absent) Dependent Binary
5 Local Invasion Invasion of the tumor into the surrounding organs (present or absent) Dependent Binary
6 Renal vein thrombosis Renal vein thrombosis (present or absent) Dependent Binary
7 Enlarged lymph nodes Enlarged perirenal lymph nodes > 1 cm in long axis, present or absent, if present, number of involved nodes.
Independent Quantitative†
8 Intratumoral Hemorrhage Areas of intratumoral hemorrhage on the precontrast scan (present/absent) Independent Binary
9 Calcifications Intratumoral calcifications (present or absent) Independent Binary
10 Pattern of enhancement Pattern of tumor enhancement involving > 50% of tumor area (homogeneous or heterogeneous)
11 Low attenuation, precontrast
phase
Attenuation on precontrast image (least attenuating area) measured with 1-cm circle
ROI
Independent Quantitative*
12 High attenuation, precontrast phase
Attenuation on precontrast image (most attenuating area) measured with 1-cm circle ROI
Independent Quantitative*
13 Low attenuation, postcontrast
phase
Attenuation on postcontrast image (least attenuating area) measured with 1-cm
circle ROI
Independent Quantitative*
14 High attenuation postcontrast
phase
Attenuation on postcontrast image (most attenuating area) measured with 1-cm
circle ROI
Independent Quantitative*
15 Attenuation change, low Attenuation on postcontrast image corresponding to same ROI measured on 11 above
Independent Quantitative*
16 Attenaution change, high Attenuation on postcontrast image corresponding to same ROI measured on 12
above measured with 1 cm circle ROI
Independent Quantitative*
17 Pattern of tumor necrosis Pattern of tumor necrosis: percentage tumor necrosis: (0%–25%, 26%–50%, 51%– 75%, 76%–100%)
Independent Ordinal
18 Tumor margins Tumor margins (sharply defined or ill defined) Independent Binary
19 Tumor contour Tumor confined within the renal contour or exophytic Independent Binary
20 Tumor shape Circumscribed versus infiltrative Independent Binary
21 Location Tumor Location: upper, mid, or lower pole Independent Ordinal
22 Involvement of renal pelvis Involvement or invasion of the tumor into the renal pelvis: present or absent Independent Binary
23 Tumor number Number of discrete, noncontiguous tumors in the target kidney Independent Quantitative†
24 Presence of distant metastasis Distant mets: presence or absence of visible lesions suspicious for metastasis with
in the visualized chest or abdomen
25 Nonintrinsic RCC features Presence of absence of findings in target kidney not directly related to the target
lesion such as renal cysts or renal stones
Independent Binary
26 Tumor transition zone Tumor transition zone: An assessment of the transition zone from tumor tissue to renal parenchyma tissue, scored as either the presence or absence of a clearly
demarcated tumor, in its entirety, with a sharply defined transition between tumor
and renal parenchyma.
Independent Binary
27 Ratio of contrast to necrosis Ratio of contrast to necrosis where “mild” has a ratio 1: Contrast enhancing volume is equal to or greater than necrosis volume, where “moderate,” the ratio is
approximately 1, and severe where the ratio is less than 1: contrast enhancing volume is significantly less than the necrosis volume
Independent Ordinal
28 Tumor heterogeneity,
precontrast phase
Tumor heterogeneity on the precontrast image is mild, moderate, or severe Independent Ordinal
29 Tumor heterogeneity,
postcontrast phase
Tumor heterogeneity on the postcontrast image is mild, moderate, or severe Independent Ordinal
30 Tumor-parenchyma interaction Tumor-parenchyma interaction: A discrete rim of enhancement partially or
completely circumscribing the tumor on equilibrium phase imaging in the absence of
a hypoattenuating rim
Independent Binary
31 Tumor-parenchyma interface Tumor-parenchyma interface: A hypoattenuating rim completely or partially circumscribing the tumor on equilibrium phase imaging.
Independent Binary
32 Internal vascularity Internal arteries: The presence or absence of discrete enhancing arteries within the
tumor
Independent Binary
34 Tumor hypoxia1 Internal arteries in areas of necrosis: The presence or absence of internal arteries in
regions of tumor necrosis
Independent Binary
35 Tumor hypoxia2 Internal arteries at the edge of regions of necrosis: The presence or absence of internal arteries extending up to the edge of a region of necrosis
Independent Binary
Note.—ROI = region of interest.
* Real numbers.
†
