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Oxidative stress produced by hyperthyroidism status induces the antioxidant enzyme transcription through the activation of the nrf 2 factor in lymphoid tissues of balb/c mice

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Figure 1: Evaluation of serum levels of thyroid hormones. The levels of T3 and T4 were determined by radioimmunoassay in serum samples from control mice (euthyroid) or treated with T4 (hyperthyroid), as described in Materials and Methods
Figure 2: Modulation of the levels of reactive oxygen species. Lymphocyte cells of euthyroid and hyperthyroid mice were incubated with 10 μM DCFH-DA for 30 min at 37 ° C, and the fluorescence intensity of the oxidized probe was measured by a flow cytometer a
Figure 3: Genomic and protein expression of antioxidant enzymes. (a) The genomic expression of CAT, GPX-1, and SOD-1 was evaluated by conventional PCR
Figure 4: Induction of ROS on the genomic expression of antioxidant enzymes. (a) Lymph nodes and spleen cells of euthyroid mice were incubated at 37 ° C in the absence or presence of H 2 O 2 (250 μM) for 4 hours and/or N-acetyl-cysteine (NAC; 2 mM) for 12
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