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Cell survival and differentiation with nanocrystalline glass-like carbon using substantia nigra dopaminergic cells derived from transgenic mouse embryos

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Fig 1. Images and characterization of nanocrystalline glass-like carbon (NGLC) films using different microscopy techniques (optical, electron microscopy and atomic force microscopy (AFM))
Fig 2. Influence of the concentration of nanocrystalline glass-like carbon (NGLC) microflakes on cell growth
Fig 3. MTT assay for cell metabolism and proliferation. SN4741 were cultured in media containing five different microflakes concentrations (0 as control, 1, 5, 10, 20 and 50 μg/ml) of nanocrystalline glass-like carbon (NGLC) for 24 h, 3 days and 7 days (A)
Fig 4. Cytotoxicity measurement via an apoptosis/necrosis assay. Values indicates apoptotic/necrotic cells expressed as percentage of positive-stained cells counted by flow cytometry after 24 hours, 3 days and 7 days of culture with nanocrystalline glass-l
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