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Angiotensin II Induced Mesangial Cell Damage Is Preceded by Cell Membrane Permeabilization Due to Upregulation of Non Selective Channels

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Figure 2. AngII increases phosphorylation of MYPT1 in MES-13 cells. Graphs showing the phosphorylation of MYPT1 evaluated by western blot analysis in MES-13 cells exposed to AngII (10 −7 M) for different times (0, 24, 48 and 72 h)
Figure 3. AngII increases the amount of Cx43, Panx1 and P2X 7 R in MES-13 cells. Graphs showing the relative amount of different Cxs (Cx40, Cx43 and Cx45 detected as single bands) (A), Panx1 detected as a doublet (B) and P2X 7 R detected as single band (C)
Figure 4. ROCK, Cx43 HC, Panx1 Ch and P2X 7 R blockers reduce the AngII-induced cell membrane permeabilization in MES-13 cells
Figure 5. AngII decreases gap junctional coupling between mesangial cells. (A) Etd + was microinjected into the brightest cell (arrow) and diffused to neighboring cells
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