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Optimization and validation of an isocratic HPLC method for caffeine, 8 chlorotheophylline and diphenhydramine determination : stress test for stability evaluation / C Barbas [et al ]

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Fig. 2. Effect of pH variation on capacity factors of analytes.
Fig. 3. Chromatograms of standards of caffeine, 8-chlorotheophylline and diphenhydramine in acetonitrile–(0.01 M H PO –triethylamine 3 4 pH, 3.0) (20:80, v / v) at a flow-rate of 1 ml / min and UV detection at 229 nm
Fig. 5. Chromatograms of 1% solutions of a pharmaceutical preparation containing caffeine, 8-chlorotheophylline and diphenhydramine as active principles submitted to oxidative stress

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