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TítuloImpact of prevalence ratios of chondroitin sulfate (CS) 4 and 6 isomers derived from marine sources in cell proliferation and chondrogenic differentiation processes

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Academic year: 2020

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Figure 2. T/C-28a2 chondrocytic cell line viability and proliferation (directly proportional to alamar  blue dye reduction) after 24, 48 and 72 h incubation in basal medium (Dulbecco’s modified Eagle’s  medium (DMEM) 10%) supplemented with different concen
Figure 3. Histological analysis for morphology (haematoxylin and eosin, HE), proteoglycans  (toluidine blue, TB) and sulfated glycosaminoglycans (GAGs) synthesis (safranin-O, SO), and  immunolocalization of collagen-type II (Col-II) and aggrecan (scale bar
Figure 4. mRNA relative expression of SOX9, ACAN, COL2A1, COL1A1, COL10A1 and IL6 from  osteoarthritic BM-MSCs pellets, after 14 days, in chondrogenic medium supplemented with 100  µg/mL CS extracted from fish (Prionace glauca, Raja clavata and Scyliorhinu
Table 1. CS-4 and CS-6 ratios and corresponding molecular weight (MW) determined for CS from  bovine and piscine sources, as previously determined
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