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Measurement of autophagy flux in the nervous system in vivo

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Academic year: 2020

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Figure 3 Rapamycin and trehalose treatment increase LC3 flux in the cerebellum. (a) Visualization of the cerebellar crest of animals injected with AAV2/2_mCherry-GFP- AAV2/2_mCherry-GFP-LC3 treated or not with rapamycin or trehalose (left panel)
Figure 4 Pharmacological activation of autophagy in the peripheral nerves. Sciatic nerves were obtained from animals transduced with AAV2/2_mCherry-GFP-LC3 and treated with trehalose or rapamycin as described in Figure 2
Figure 5 LC3 flux in the peripheral and CNS in models of mechanical injury. (a) Schematic diagram of the method and strategy employed to deliver AAV2/2_mCherry- AAV2/2_mCherry-GFP-LC3 by ICV followed by the challenge of mice with SCI or peripheral nerve cr

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