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Apoptosis, necrosis and autophagy are influenced by metabolic energy sources in cultured rat spermatocytes

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Academic year: 2020

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Fig. 1 The effect of metabolic energy sources on spermatocyte cell death. Spermatocytes were cultivated between 0 and 30 h in KHB-Lac, -Glu, -DOG or w/oS.
Table 2 Influence of metabolic energy sources on cell death rate Condition Velocity (% death/h) SD Significant respectto 0 (p \ 0.05) Lac 0.32 0.16 No Glu 0.69 0.23 Yes w/oS 0.94 0.26 Yes DOG 1.10 0.25 Yes
Fig. 3 Glucose induces apoptosis in cultured spermatocytes. a Necro- Necro-sis was determined by measuring LDH activity between 0 and 30 h in the supernatant of each culture supplemented with 5 mM Lac (circles, red line), 5 mM Glu (squares, blue line) or w
Fig. 4 Caspase activation in spermatocytes cultured without lactate. Caspase activity was evaluated by colorimetric assays
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