• No se han encontrado resultados

Supplementary Figure 1

N/A
N/A
Info
Descargar
Protected

Academic year: 2023

Share "Supplementary Figure 1"

Copied!
7
0
0

Cargando.... (Ver texto completo ahora)

Descargar ahora ( 7 página )

Texto completo

(1)

NeuN (A)

PI A PI A

PI A

a b

c

e f

g

d

2C

4C

4C 2C

Epilepsy Control

Epilepsy Control h

S100β+

NeuN-

S100β+

NeuN+

S100β- NeuN-

S100β- NeuN+

S100β+

NeuN-

S100β+

NeuN+

S100β- NeuN-

S100β- NeuN+

S 10 0 β (A ) S 10 0 β (A )

NeuN (A)

S S C A

FSC A

S S C A

PI A PI A

P I H P I W

(2)

IP+ -E 12 H AF 488+AF 647+IP_34

AF488+

AF488+

AF488+

AF488+

AF488+

AF488+

1e5 1e6

1e4

Intensity_MC_Ch02-AF 488 1,2

0,9

0,3 1,5

0,6

0

Normalized Frequency

Intensity_MC_Ch02-AF 488

Population Count %Gated Mean CV

IP+ & Cells & Single & Focus 455 100 103628,62 120,4

AF488+ & IP+ & Cells & Single & Focus 359 78,9 119235,96 91

AF488+ -E 12 H AF 488+AF 647+IP_34

Neuron 4c Neuron 4c Neuron 4c Neuron 4c Neuron 4c Neuron 4c

2e5 4e5

Intensity_MC_Ch05-IP 2

1 3

0

Normalized Frequency

Intensity_MC_Ch05-IP

Population Count %Gated Mean CV

AF488+ & IP+ & Cells & Single & Focus 359 100 238466,81 27,11 Neuron 4c & AF488+ & IP+ & Cells & Single & Focus 19 5,29 427290,32 2,528

IP+ -E 12 H AF 488+AF 647+IP_34

AF647+

AF647+

AF647+

AF647+

AF647+

AF647+

1e6 1e5

1e4

Intensity_MC_Ch11-AF 647 1

2

1,5

0,5

0

Normalized Frequency

Intensity_MC_Ch11-AF 647

Population Count %Gated Mean CV

IP+ & Cells & Single & Focus 455 100 1265970,65 54,98

AF647+ & IP+ & Cells & Single & Focus 447 98,2 1230183,3 52,58

AF647+ -E 12 H AF 488+AF 647+IP_34

Astroc 4c Astroc 4c Astroc 4c Astroc 4c Astroc 4c Astroc 4c

4e5 2e5

Intensity_MC_Ch05-IP 2

1 3

0

Normalized Frequency

Intensity_MC_Ch05-IP

Population Count %Gated Mean CV

AF647+ & IP+ & Cells & Single & Focus 447 100 230440,03 25,77 Astroc 4c & AF647+ & IP+ & Cells & Single & Focus 27 6,04 423407,33 4,869

No rmaliz ed F requ ency No rmaliz ed F requ ency No rmaliz ed F requ ency No rmaliz ed F requ ency

e f

h g

647+IP_34

Cells Cells Cells Cells Cells Cells

600

0 300

Area_M09 1e7

1e4 1e5 1e6

Intensity_MC_Ch06

Area_M09, Intensity_MC_Ch06

Population Count %Gated Mean CV

Single & Focus 1638 100 173,89, 684360,74 36,54, 139,27 Cells & Single & Focus 822 50,2 178,7, 134513,57 31,25, 63,96 a

2 1

2 1

NeuN PI

Bright field

NeuN PI

Bright field b

c d

No rmaliz ed F requ ency

Area_M 09

Area_M 01

Gradient RMS M01 Channel 01

A spect Rat io_M 01 Int ensit y _M C -Ch ann el 06

NeuN-Alexa 488 DNA content PI

DNA content

S100β-Alexa 647

(3)

PercentageoftetraploidNDRG2+ astrocytes c a

Percentageof tetraploidNeuN+ neuronsPercentageof tetraploidcells

p= 0.577

EZ CSZ

EZ CSZ

EZ CSZ

EZ CSZ

EZ CSZ

p= 0.197

p= 0.502 p= 0.497

p= 0.497 e

PercentageoftetraploidS100β+ astrocytes b

d

PercentageoftetraploidCTIP2+ neurons

(4)

p = 0.965

c

a b

d

Percentageof tetraploidcells

Percentageof tetraploidcells

Control Epilepsy Control EZ

Percentageof tetraploidcells Percentageof tetraploidcells

Control CSZ Control Epilepsy

p = 0.048

p = 0.212 p = 1.000

*

(5)

p = 0.185

c

a b

Percentageoftetraploidneurons

Percentageoftetraploidneurons

Control Epilepsy Control EZ

Percentageoftetraploidneurons

Control CSZ

p = 0.429

p = 0.400

(6)

p = 0.051

Control Epilepsy Control EZ

Control CSZ Control Epilepsy

p = 0.905

p = 0.152

p = 0.133

Percentageof tetraploidastrocytes

Percentageof tetraploidastrocytesPercentageof tetraploidastrocytes Percentageof tetraploidastrocytes

a b

c d

(7)

% 4C S100β Hippocampus

% 4C NeuN Cortex

% 4C S100β Cortex

% 4C NeuN Amydala

% 4C S100β Amygdala

Nº Anticonvulsant drugs

Onset Age

% 4C S 10 0 β Hip p o ca mp u s

% 4C NeuN Co rt ex

% 4C S 10 0 β Co rt ex

% 4C NeuN A my d ala

% 4C S 10 0 β A my g d ala

Nº A n ticon v u lsa n t d ru g s

O n se t A g e % 4C NeuN Hip p o ca mp u s

Age

% 4C S 10 0 β Hip p o ca mp u s

% 4C NeuN Cor te x

% 4C S 10 0 β Co rt ex

% 4C NeuN A my d ala

% 4 C S 1 0 0 β A my g d ala % 4C NeuN Hip p o ca mp u s

Number of seizures

a

b

Referencias

Descargar ahora ( PDF - 7 página - 671.77 KB )

Documento similar

MicroRNA-21 links epithelial-to-mesenchymal transition and inflammatory signals to confer resistance to neoadjuvant

A further analysis of an additional cohort of 30 HER2-positive breast cancers (Supplementary Table 1) confirmed that miR-21 overexpression was associated with RD ( p =

ON THE USE OF LAYERED DOUBLE HYDROXIDES IN THE MANAGEMENT OF 129I FROM LIQUID NUCLEAR WASTES Luis Iglesias Pérez Dipòsit Legal: T 893-2015

Figure 1-1 – Nuclear Power Reactors distribution in the world. 4 Figure 1-2 – Penetration power of alpha, beta and gamma radiation. 7 Figure 1-3 – Advertisements of products

Supplementary material

29 Research group Website representing a research group, regardless the status (public or private) and the parent institution (e.g., https://www.pocog.org.au). 6

Supplementary material

11 Figure S4 Kaplan-Meier estimates of progression-free survival (A) and overall survival (B) according to tumour sidedness in patients with BRAF- and/or PIK3CA mutations (n=44)...

Supplementary Materials

Figure S1. Low NO induces HIF-1α accumulation in human ESCs in normoxia. A) Analysis of the HIF-1α protein expression by Western blotting. As load control β-ACTIN was

Supplementary Information

(f) Representative amperometric recording of the secretory activity induced by hypercapnia (20% CO 2 , pale red) 10 mM lactate (grey) and high potassium on chromaffin cells in

Feature Selection Stability and Accuracy of Prediction Models for Genomic Prediction of Residual Feed Intake in Pigs Using Machine Learning

Supplementary Figure 1 | Boxplots for the Spearman correlation for average daily gain obtained in 10-outer testing sets with a Genomic Best linear Unbiased Predictor

Supplementary Material

Adjusted for age, center, education, family history of breast cancer, menarche, number of children, BMI, contraceptive use, hormonal replacement therapy and menopausal