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Quality assurance of natural products (Fifth Year 2020/2021)

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Course Specification

Quality assurance of natural products (Fifth Year 2020/2021)

University: Minia Faculty: Pharmacy

Department: Pharmacognosy 1- Administrative Information Course Title: Quality assurance of natural products

Code: PG517 Academic Year/level: Fifth year 2020-2021

Department(s) offering the course:

Pharmacognosy

Lecture: 2 hrs/week (48 hrs/year) Practical: 3 hrs/week (72 hrs/year) Total: 120 hrs/ year

2- Aims After completion of this course, the student should have information about qualitative and quantitative analysis of raw materials, natural products and herbal medicines, in addition to chromatographic techniques and its application in isolation of active principles.

3- Overall aims of Course:

A- Knowledge and Understanding

By the end of the course, the student should be able to:

a1- Identify clearly how the spectral analyses used in determination of the structures of natural products.

a2- Identify the modern techniques of chromatography used in separation of plant constituents and their quality control.

a3- Recognize how to investigate chemical constituents in medicinal plants by modern techniques.

a4- Recognize obviously quality control, evaluation of crude drugs and their pharmacopoeial constants.

a5- Recall the proper drying and storage conditions of medicinal plants to keep their maximum effectiveness.

B- Intellectual Skills: By the end of the course, the student should be able to:

b1- Apply deeply the scientific thinking and use the studied information properly in the applied fields.

b2- Inspect and solve properly the scientific problems in the field of herbal drugs.

b3-Solve problems concerning qualitative and quantitative determination of active constituents in medicinal plants and in herbal preparations.

b4- Compare appropriately between correct and wrong data of natural active constituents reported in different websites.

C- Professional and Practical Skills:

By the end of the course, the student should be able to:

c1- Apply different chromatographic methods for isolation of plant constituents.

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c2- Use efficiently microscopical, chromatographic and spectral techniques to detect adulteration of raw plants.

c3- Carry out quality control tests of herbs using UV

spectrophotometers and various chromatographic techniques.

D- General and transferable skills:

By the end of the course, the student should be able to:

d1- Update effectively his/her knowledge concerning herbal formulations and their quality control.

d2- Plan and manage successfully a scientific project.

d3- Lead and work creatively in a team or independently.

d4- Search continuously for new techniques for analysis and isolation of herbal active constituents

4- Course Contents:

1- First term A- Lectures topics

Weeks No. of hours

Topic

6 12

Chromatography & its applications in natural products:

- Introduction

- Column Chromatography - Paper Chromatography - Thin layer Chromatography - Ion exchange Chromatography - Size exclusion Chromatography - Affinity Chromatography

- Supercritical fluid Chromatography - Droplet counter current Chromatography - Gas Chromatography

- HPLC/LCMS - Detectors

- Sampling techniques :- Head space,

Solid Phase Extraction & Solid Phase Micro extraction

4 8

Spectral analyses of naturally isolated compounds:

- UV spectroscopy - IR spectrometry - Mass spectrometry

- Proton- and carbon-NMR spectroscopy - DEPT

2 4

Quality control of herbal drugs:

- Introduction of quality control of herbal drugs.

- Types of adulterations. Evaluation of medicinal crude drugs, including:

- I-Organoleptic examination.

- II-Botanical characters.

- III-Physical examination.

- IV-Chemical examination.

- V-Biological examination.

12 24

Total

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3/6 B- Practical topics

Weeks No. of hours

Topic

1 3

- Introduction of chromatography

1 3

-Thin layer chromatography

1 3

-Column chromatography

2 6

-Paper chromatography

1 3

- UV, IR and Mass spectrometry

1 3

- UV problems

2 6

- 1H- and 13C-NMR spectroscopy

1 3

- NMR problems

2 6

- General problems

Practical exam

12 36

Total

Assessment Schedule: Assessment 1: assignment evaluation in the 12th week Assessment 2: practical exam in the 13th week Assessment 3: written exam in the 14th week Assessment 4: oral exam in the 14th week

Weighting of Assessment: Exam Mark %

1st term final examination 80 53.3 1st term oral examination 20 13.3 1st term practical examination 40 26.6

Midterm exam 10 6.6

Total 150 100

8- List of References:

A- Course Notes 1- Quality assurance of natural products department notes.

2- Printed PowerPoint slides

B- Essential Books: 1- Trease & Evans. Pharmacognosy. W.B. Saunders Company, January 24th 2002.

2- E.P. Claus and V.E. Tayler. Pharmacognosy. Philadelphia, PA: Lea & Fibiger., 5th ed., 1965

3- Egyptian Pharmacopoeia, (English Texts).

C- Recommended Books: 1- J. Long. The Gale Encyclopedia of Alternative Medicine, Gale Group; 2nd edition, December 2004.

2- V. Schulz, R. Hänsel, M. Blumenthal, and V. E.

Tyler, Rational Phytotherapy. A Reference Guide for Physicians and Pharmacists, Springer, Berlin, Germany, 2004.

D- Periodicals, Websites: 1- http://www.chemweb.com/

2- http://www.ncbi.nlm.nih.gov/pubmed/

3- J. of Phytochemistry.

4- J. of Planta Medica.

5- J. of Chem. Pharm. Bull.

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6- J. of Natural Products.

7- J. of Die Pharmazie.

Course Coordinator:

Prof. Samar Y. Desoukey

Head of Department:

Ass. Prof. Ashraf Nageeb Elsayed Hamed

Matrix I- Coverage of the course ILOs by different topics

Topics

A-

Knowledge and

Understand ing

B- Intellectual skills

C-

Professional and

practical skills

D- General and transferable skills

a1 a2 a3 a4 a5 b1 b2 b3 b4 c1 c2 c3 d1 d2 d3 d4 Chromatography &

its applications in natural products

Spectral analyses of naturally isolated compounds

Quality control of

herbal drugs

Assignment

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Matrix II- Coverage of the course ILOs by different learning methods

Teaching & learning methods

A-Knowledge and Understandin

g

B- Intellectual

skills

C- Professio

nal and practical

skills

D- General and transferabl

e skills a1 a2 a3 a

4 a 5

b 1

b 2

b 3

b 4

c 1

c 2

c 3

d 1

d 2

d 3

d 4

Lecture ● ●

Practical ● ● ●

Assignment ● ● ●

Matrix III- Measuring the achievement of the course ILOs by different assessment method

Assessment methods

A-

Knowledg e and Understa nding

B- Intellectual

skills

C- Professio

nal and practical

skills

D- General and transferable

skills

a 1

a 2

a 3

a 4

a 5

b 1

b 2

b 3

b 4

c 1

c

2 c3 d 1

d 2

d 3

d 4

Written ● ● ● ● ● ● ● ● ● ● ● ● ●

Oral ● ● ● ● ● ● ● ● ● ●

Practical ● ● ● ● ●

Assignment ● ● ● ●

Referencias

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