1612 Journal of Applied Sciences Research, 8(3): 1612-1614, 2012
ISSN 1819-544X
This is a refereed journal and all articles are professionally screened and reviewed
O RIGINAL A RTICLES
Corresponding Author: Córdova-Izquierdo Alejandro, Departaemnto de Producción Agrícola y Animal. Universidad Autónoma Metropolitana Unidad Xochimilco. Calz. Del hueso 110 Col. Villa Quitud C.P.
04960, México, D.F.
E-mail: [email protected]
Effect Of Cloprostenol And Fluorogestone Acetate More PMSG On Synchronization And No Return To Estrus In Seasonal Anestrus Dorper Sheep
1Córdova-Izquierdo Alejandro, 1Xolalpa-Campos Víctor Manuel, 1Ruiz-Lang Claudio Gustavo,
1Espinosa-Cervantes Román, 2Córdova-Jiménez Cristian Alejandro, 3Méndez-Mendoza Maximino, 3Huerta-Crispín Rubén, 3Villa-Mancera Abel, 4Juárez-Mosqueda Ma. de Lourdes,
5Sánchez-Aparicio Pedro and 6Guerra-Liera Eulogio
1Departaemnto de Producción Agrícola y Animal. Universidad Autónoma Metropolitana Unidad Xochimilco.
Calz. Del hueso 110 Col. Villa Quitud C.P. 04960, México, D.F.
2Facultad de Veterinaria. Universidad de León, España.
3Facultad de Veterinaria. Benémerita Universidad Autónoma de Puebla, México.
4Departamentod e Morfología. FMVZ-UNAM.
5DGIF-SENASICA.
6Facultad de Agronomía. Universidad Autónoma de Sinaloa, México.
ABSTRACT
Synchronization of estrus allows for an increase of offspring per year, as it reduces the calving interval and can be handled regardless of the season. It also allows the time of delivery be uniform throughout the animal production unit (APU). The aim of this study was to evaluate the effect of cloprostenol and more flurogestona aceteto of PMSG on synchronization and not return to estrus in anestrus seasonal Dorper sheep. We evaluated two groups of 20 sheep with two treatments: T1 = Flurogestona effect of acetate (FGA) with 40 mg on day zero + PMSG on day 9. T2 = PGF 2 (cloprostenol) using two doses with an interval of 10 days between doses. Estrus detection was performed by direct observation of the sheep using the AM / PM for 40 minutes after the scheduled start of appearance of behavioral estrus, as well as the presence of the stallion. For the observation of no return to estrus sheep were exposed to the stallion for 16-19 days after the service, taking as positive the sheep that were not mounted. The results of the two treatments were: T1 = the total number of sheep that had obvious estrus was 90% and only 10% had no estrus. 100% of females served, it was found that the pregnancy rate was 80%. T2 = Shows a total of 70% of females with obvious signs of estrus, with the remainder (30%) that was introduced. Differences between groups were not significant (P> 0.05). In conclusion, the use of progestogens in combination with gonadotropins is more effective than single use of PGF2a for synchronization and non-return to estrus in seasonally anestrus ewes.
Key words: Estrus synchronization. Anestrus dorper sheep. Flurogestona acetate. PMSG.
Introduction
The nature of seasonality in sheep production prevents the producers guarantee a steady source of product, milk or meat to consumers. Manage the breeding season in sheep has become one of the most important benefit of which is to obtain homogeneous part and thus year-round availability of animals for the market (Letelier et al., 2003).
One of the factors limiting sheep productivity is the influence that climate has on reproduction in this species. This phenomenon is common in countries located geographically north and south of the tropics of Cancer and Capricorn, respectively, which is lost gradually with proximity to Ecuador (Navarro and Torres, 1994, Letelier et al., 2003).
According to their reproductive behavior of mammals are classified as seasonal breeders or continuous, according to cycle at a certain time or throughout the year. In the first group and as is the breeding season, fall seasonal photoperiod in ascending or descending. Based on this classification, breeding sheep are considered seasonal photoperiod polyestrous descending (Ruiz et al., 2001).
The use of hormonal products to improve the reproductive performance of herds is an alternative, as these allow the synchronization of oestrus, for natural mating or artificial insemination, whose main benefits are: a group of birth, weaning and programming sale of animals for uniform lots. One way to synchronize the estrous
1613 J. Appl. Sci. Res., 8(3): 1612-1614, 2012
cycle in sheep is by using natural progesterone treatments with intravaginal CIDR device or intravaginal sponges (Estrella et al., 2000, Molina et al., 2005).
The aim of this study was to evaluate the effect of cloprostenol and acetate Flurogestona + PMSG on the percentage of synchronization and no return to estrus in anestrus seasonal Dorper sheep.
Material and Methods
The study was conducted at a Sheep Production Unit in the State of Mexico. Used 40 Dorper ewes 2 years of age, housed, whose diet is based on oat hay and concentrate feed to 11% protein.
The animals were divided into two treatments: treatment 1, with 20 sheep were fitted with intravaginal sponges with 40 mg of fluorogestone acetate (MGA) at 9 days were given 500 IU pregnant mare serum gonadotropin (PMSG) , day 11 and sponges were removed from day 12 was performed estrus detection, using a breeding male, which gave them service by natural breeding.
In Treatment 2 was administered Cloprostenol a group of 20 sheep were used two doses of 170 mg intramuscularly at an interval of 10 days, estrus detection was performed 24 hours later with a male player and the service is out with natural mating.
The variables measured in both treatments was the timing and percentage return to estrus. He did not return to estrus was assessed by exposing females to male between 16 and 19 after mating and were used as non-return to estrus, females did not accept the male.
Statistical analysis was performed using the software of the Autonomous University of Nuevo León (DISESTAD) Olivares, 1990, we determined the coefficient of variation and performed the Tukey test for comparison of means.
Results:
The results are presented in the following table:
Results for both treatments (P> 0.05).
Treatment % synchronization % no return to estrus
FGA + PMSG 90 80
Cloprostenol 70 75
Discussion:
The estrus synchronization rate obtained in this work are higher than those reported by Gonzales and Chavez (2005), which show percentages of 75%, this is because they only applied PMSG and in this study was the first treatment with fluorogestone acetate + PMSG with which the efficacy was higher as fluorogestone acetate causes a luteal phase and to apply and remove sponges PMSG, ovulation occurs thus favors the presence of estrus in sheep (Mejia et al., 1998). In addition, according to Cordoba et al. (1999), progestogens are ineffective in anestrus animals, however, efficiency is increased when used in combination with other hormones such as gonadotropins extrahipofisiarias as hCG) and PMSG.
The results of this study are consistent with those reported by Knight et al. (2000), who mentions that in ewes treated with implants of progesterone (CIDR), the percentage of estrus for 5 days before they were in contact with the male, this because the implants removed after lower levels of progesterone and estrogen increase thereby making the expression of estrus. In the same study reported that 30% of estrus occurred in the first 24 hours, 20% at 36 hours, 15% at 48 hours and 10% at 60 hours. The results of the estrus synchronization rate are also consistent with those given by Star et al. (2000), which was used progesterone (P4) and vitamin E (VE) intramuscularly.
Other studies applying fluorogestone acetate and PMSG were obtained very similar results to those obtained in this work, Córdova et al. (1999) reports that seasonal anestrus in sheep (May-June), 25% of treated ewes showed estrus 24 hours after applying the treatment, 48 hours, 20.8% and 50% within 72 hours. Likewise, Cline et al. (2001) also reported positive results using the combination of progestin and gonadotropins.
The estrus synchronization rate is also consistent with those reported by Richardsond et al. (2002), which was 90.6% which does not vary 90% obtained in this work.
The percentage of non-return to estrus, which was taken as a positive pregnancy was 80 and 75% for treatment 1 and 2, respectively, which means a high percentage compared to that obtained by Muñoz et al.
(2002) , which reported 24.1% pregnancy rate.
Muñoz et al. (2002), reported 61.4% of sheep bred who repeated estrus, but in this study were were higher because natural mating is used which is more efficient than artificial insemination.
Importantly, the work was done near the end of the breeding season, from May to July, which determines a lower fertility potential due to irregular estrous cycles (Gigli et al., 2006).
1614 J. Appl. Sci. Res., 8(3): 1612-1614, 2012
In conclusion, the two treatments evaluated in this study were shown to be effective and while there was no statistically significant differences, the use of progestins in combination with gonadotropins, is more effective than the use of prostaglandins for synchronization alone and not return to estrus seasonal anestrus in sheep.
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