Now I need to estimate the predictive value of the ten common genes using Random Forest in R, anyone knows how the data frame should be?

1 year ago 5 Replies
HA
Hiba Almaadanli
1 year ago

Hi,
I have RNAseq matrix counts for two datasets. I conducted DEGs then the intersection between DEGs lists was done. Now I need to estimate the predictive value of the ten common genes using Random Forest in R, anyone knows how the data frame should be? Colunms= ten genes
Rows = case and controls?

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Adrián Calleros 1 year ago

https://r4ds.had.co.nz/tidy-data.html

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Wisam Saleem 1 year ago

When you conducted the DEGs, have you got any p-values? What method do you use? Is it DESeq2 for example.

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Hiba Almaadanli 1 year ago

Wisam Saleem yeah I used DESeq2, and it includes the p-value in the results

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Wisam Saleem (1 year ago)

Hiba Almaadanli. OK, I think I have the codes for this exactly by random forest algorithm. I can't put it in public because it's related to private repository for research paper.

Hiba Almaadanli (1 year ago)

Wisam Saleem Thanks, I have sent a message to you