HeLa cells
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HOTAIR Knockdown Decreased the Activity Wnt/ß-Catenin Signaling Pathway and Increased the mRNA Levels of Its Negative Regulators in Hela Cells
... in HeLa cells while it is diminished in C-33A and SiHa cells ...in HeLa cells and increased in C-33A cells ...cancer cells, suggesting a plausible role of HOTAIR in TET1 ...
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Modulation of Ca2+ release and Ca2+ oscillations in HeLa cells and fibroblasts by mitochondrial Ca2+ uniporter stimulation
... oscillations. The two compounds, the synthetic oestrogen receptor agonist PPT and the flavonoid kaempferol, have completely different molecular structures, but they similarly activate MCU (Montero et al. 2004; Lobat ´on ...
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Extracellular gentamicin reduces the activity of connexin hemichannels and interferes with purinergic Ca2+ signaling in HeLa cells
... Gap junction channels (GJCs) and hemichannels (HCs) are composed of protein subunits termed connexins (Cxs) and are permeable to ions and small molecules. In most organs, GJCs communicate the cytoplasm of adjacent ...
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Cation permeation through connexin 43 hemichannels is cooperative, competitive and saturable with parameters depending on the permeant species
... type HeLa cell transfectants (Cx43-EGFP- and Cx43-WT-HeLa cells, respectively) were used. Levels of Cx43-EGFP at the cell periphery and rate of dye uptake were directly related. The rate of uptake of ...
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The mitochondrial Na+/Ca2+ exchanger plays a key role in the control of cytosolic Ca2+ oscillations
... non-excitable cells. In HeLa cells, inhibition of the mitochondrial Na + /Ca 2+ exchanger shifted the pattern from high-frequency irregular oscillations to baseline spikes and in fibroblasts ...
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Absence of p.R50X Pygm read-through in McArdle disease cellular models
... in HeLa cells 72 h after transfection with ...in cells transfected with ...in HeLa cells. Thus, cells were treated for 72 h with either amlexanox, Ataluren, RTC13 or G418 at ...
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![Monitoring mitochondrial [Ca2+] dynamics with rhod 2, ratiometric pericam and aequorin](https://data02.123doks.com/thumbv2/123dok_es/000/549/549230/cover.webp)
Monitoring mitochondrial [Ca2+] dynamics with rhod 2, ratiometric pericam and aequorin
... the cells present in the microscope field (13 and 16) in two different ...(B) HeLa cells expressing mitRP were stimulated with 100M histamine as ...single cells. The lower trace is the mean of ...
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New approaches to the study of mitochondrial Ca2+ dynamic
... Similarly, panel F shows that measurements in HeLa cells loaded with pericam and perfused with a 10 M Ca2+ buffer also show an increase in mitochondrial [Ca2+ ] in the presence of FCCP.[r] ...
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Critical role of the first transmembrane domain of Cx26 in regulating oligomerization and function
... ABSTRACT To identify motifs involved in oligomerization of the gap junction protein Cx26, we studied individual transmembrane (TM) domains and the full-length protein. Using the TOXCAT assay for interactions of isolated ...
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Syndecan 4 inhibits Wnt/ß catenin signaling through regulation of low density lipoprotein receptor related protein (LRP6) and R spondin 3
... D) HeLa cells were cotransfected with a mixture of siSdc4 and siFn to potentiate Wnt/-catenin signaling in response to ...(E) HeLa cells were cotransfected with LRP6, which activates ...
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Linoleic acid induces opening of connexin26 hemichannels through a PI3K/Akt/Ca2+ dependent pathway
... results, HeLa cells express a free fatty acid receptor, but to our knowledge it remains uncharacterized, and could mediate activation of a PI3K/Akt-dependent ...
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Efecto de hotair en la metilación global del DNA en la línea celular HeLa
... Long non-coding RNAs (lncRNAs) participate in the origin and development of different diseases, for example, cancer. HOTAIR is an lncRNA involved with many types of cancer, including cervical cancer, in this type of ...
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Efecto de HOTAIR sobre TET1 y la vía WNT en la línea celular HeLa.
... Background/Aims: HOTAIR is a long non-coding RNA that promotes the development of human cancer. TET1 enzyme is involved in DNA demethylation by oxidation of 5-methylcytocine and it is considered a tumor suppressor in ...
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TítulomiR 203 regulates cell proliferation through Its influence on Hakai expression
... in HeLa cells, which did not express E- cadherin, as shown in Figure S1 and previously published ...in cells transfected with pre-miR-203, compared to cell transfected with scrambled control miRNA ...
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Ca2+ homeostasis in the endoplasmic reticulum measured with a new low Ca2+ affinity targeted aequorin
... (TMRE). HeLa cells were mounted in a cell chamber in the stage of a Zeiss Axiovert 200 microscope under continuous ...perfusion. Cells were then permeabilized with digitonin and perfused with ...
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Nucleotide P2Y(1) receptor regulates EGF receptor mitogenic signaling and expression in epithelial cells
... resting cells into G1, and then a second stimuli-dependent process, which stabilizes components responsible for surpassing the restriction point that takes place 7-9 hours later (Jones and Kazlauskas, ...in ...
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Sonic Hedgehog modulates EGFR dependent proliferation of neural stem cells during late mouse embryogenesis through EGFR transactivation
... in HeLa cells show that Hedgehog pathway activation leads to EGFR internalization and to transient activation of the MAPK/ERK signaling cascade (Figure 1D, Figure S2), without detectable ...
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Estudio del fenotipo de una línea celular hela FASTK KO
... A pesar de haberse construido los cuatro vectores lentivirales, con los dos genes y sus correspondientes marcajes, sólo dos de estos vectores infectan las células HeLa – FASTK – KO. Los plásmidos que han infectado ...
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Bioregenerative Engineering Principles and Applications – Shu Q. Liu.
... eukaryotic cells in many aspects, but there are ...eukaryotic cells is much ...mammalian cells. Second, eukaryotic cells contain usually multiple ...These cells must conduct DNA ...
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