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Polymerase chain reaction

A rapid and sensitive diagnosis of bovine leukaemia virus infection using the nested shuttle polymerase chain reaction

A rapid and sensitive diagnosis of bovine leukaemia virus infection using the nested shuttle polymerase chain reaction

... RESUMO.- [Diagnóstico rápido e sensível da infecção com o vírus da Leucemia Bovina através de Shuttle Nested Polymerase Chain Reaction.] O Vírus da leucemia bovina (BLV) é o agente causal da Leucose ...

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Genes that encode botulism neurotoxins A, B, E and F in Neotropical bee honey identified with the Polymerase Chain Reaction

Genes that encode botulism neurotoxins A, B, E and F in Neotropical bee honey identified with the Polymerase Chain Reaction

... Abstract: Honey can be used for the treatment of wounds, sores and skin burns, but it might be contaminated with Clostridium botulinum spores. In order to evaluate Costa Rican raw honey samples, the detection of ...

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The role of polymerase chain reaction of high-risk human papilloma virus in the screening of high-grade squamous intraepithelial lesions in the anal mucosa of human immunodeficiency virus-positive males having sex with males.

The role of polymerase chain reaction of high-risk human papilloma virus in the screening of high-grade squamous intraepithelial lesions in the anal mucosa of human immunodeficiency virus-positive males having sex with males.

... the polymerase chain reaction (PCR) technique (GeneAmp PCR System 9700, Applied Biosystems, Roche), and for cytology using the ThinPrep Pap Test (Thin Prep Proces- sor 2000, Hologic ...

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Allele-specific polymerase chain reaction for the detection of Alzheimer ’s disease-related single nucleotide polymorphisms

Allele-specific polymerase chain reaction for the detection of Alzheimer ’s disease-related single nucleotide polymorphisms

... Background: The incidence of Alzheimer ’ s disease, particularly in developing countries, is expected to increase exponentially as the population ages. Continuing research in this area is essential in order to better ...

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Identification of blood meal source and infection with Trypanosoma cruzi of Chagas disease vectors using a multiplex cytochrome b polymerase chain reaction assay

Identification of blood meal source and infection with Trypanosoma cruzi of Chagas disease vectors using a multiplex cytochrome b polymerase chain reaction assay

... Long-term control of triatomine bugs in Chagas endemic regions will depend on a full understanding of vec- tor–parasite–host interactions. Herein we describe a cytochrome b multiplex polymerase chain ...

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Confirmation of presumptive Salmonella colonies contaminated with Proteus swarming using the Polymerase Chain Reaction (PCR) method

Confirmation of presumptive Salmonella colonies contaminated with Proteus swarming using the Polymerase Chain Reaction (PCR) method

... ABSTRACT. In México, zero tolerance regulation is practiced re- garding Salmonella in food products, the presence of which is ver- ified by the procedure described in NOM 114-SSA-1994. During the period between August ...

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Hyperspectral images and polymerase chain reaction (PCR) for the detection of allergen (peanuts traces) in powder foods.

Hyperspectral images and polymerase chain reaction (PCR) for the detection of allergen (peanuts traces) in powder foods.

... Jacxsens, L., Devlieghere, F., Debevere, J. (2002). Predictive modelling for packaging design: equilibrium modified atmosphere packages of fresh-cut vegetables subjected to simulated distribution chain. ...

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Occult hepatitis B in mexican patients with HIV, an analysis using nested polymerase chain reaction

Occult hepatitis B in mexican patients with HIV, an analysis using nested polymerase chain reaction

... Briefly, 5 µL of HBV DNA were added to 45 µL of reac- tion mixture, containing 1x PCR buffer, 0.5 µM of each primer, 75 µM dNTP’s (dATP, dGTP, dCTP and dUTP), 1.5 mM MgCl 2 , 5 U uracil DNA-glycosylase and 5 U Taq ...

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... transcriptase-polymerase chain reaction- restriction fragment length polymorphism) and the nucleotide sequences of the coat protein (CP) genes amplified to assess the presence and genetic diversity ...

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Identificacin molecular de bacterias causales de sepsis neonatal mediante la reaccin en cadena de la polimerasa (PCR)

Identificacin molecular de bacterias causales de sepsis neonatal mediante la reaccin en cadena de la polimerasa (PCR)

... Neonatal sepsis is a worldwide public health problem in many newborns. Its incidence is estimated at 8 of 1000 alive births; however the risk of morbility and mortality reaches 50 %. Neonatal sepsis presents with signs ...

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Serosurvey Study of Toscana Virus in Domestic Animals, Granada, Spain

Serosurvey Study of Toscana Virus in Domestic Animals, Granada, Spain

... Toscana virus (TOSV) is transmitted by infected sandflies. In Mediterranean countries, TOSV is one of the major viral pathogens involved in aseptic meningitis and meningoencephalitis in humans. It remains unclear if ...

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Utilidad clnica de la reaccin en cadena de la polimerasa anidada para el diagnstico de tuberculosis extrapulmonar

Utilidad clnica de la reaccin en cadena de la polimerasa anidada para el diagnstico de tuberculosis extrapulmonar

... as polymerase chain reaction (PCR) has focused WKHDWWHQWLRQRILQYHVWLJDWRUVWRZDUGWKLVGLUHFWLRQ7KH FRQFHSWRIVSHFLÀFJHQHWLFPDWHULDODPSOLÀFDWLRQDWGH- WHFWDEOHOHYHOVZRXOGEHYHU\DWWUDFWLYH7KHXVHRI3&5 ...

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Reacción en cadena de la polimerasa en fluidos oculares en las formas atípicas de uveítis posterior

Reacción en cadena de la polimerasa en fluidos oculares en las formas atípicas de uveítis posterior

... of polymerase chain reaction in ocular fluids from patients with serum serological titers positive for toxoplasma and atypical presentations of posterior ...which polymerase chain ...

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Amplified 16S Ribosomal DNA Restriction Analysis for Identification of Avibacterium paragallinarum

Amplified 16S Ribosomal DNA Restriction Analysis for Identification of Avibacterium paragallinarum

... a polymerase chain reaction (PCR), referred to as amplified 16S ribosomal DNA restriction analysis (ARDRA), was designed to identify 19 Avibacterium paragallinarum strains isolated from infraorbital ...

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The role of Interleukin 28B gene polymorphism in Turkish patients with hepatocellular carcinoma

The role of Interleukin 28B gene polymorphism in Turkish patients with hepatocellular carcinoma

... using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay in a hospital-based case-control study of 187 con- firmed HCC patients and 208 cancer-free controls of ...

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Nasopharyngeal bacterial load as a marker for rapid and easy diagnosis of invasive pneumococcal disease in children from Mozambique.

Nasopharyngeal bacterial load as a marker for rapid and easy diagnosis of invasive pneumococcal disease in children from Mozambique.

... Diagnosis of IPD remains challenging in children, as it requires collection of invasive sam- ples and the use of sensitive methods to detect S. pneumoniae in small sample volumes [9]. Cul- ture-based methods on sterile ...

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Rat DNA polymerase beta substitutes the repairing activity of DNA polymerase I in the lethal effect of UV light

Rat DNA polymerase beta substitutes the repairing activity of DNA polymerase I in the lethal effect of UV light

... ABSTRACT. The aim of this work was to search if the rat DNA polymerase beta can substitute the capability of DNA polymerase I to repair damage caused by the UV light in Escherichia coli. The oriC origin of ...

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An interpretation of the oligomerization of amino
				acids under prebiotic conditions

An interpretation of the oligomerization of amino acids under prebiotic conditions

... Let us go again to the experimental result related to the synthesis of tyrosine containing trimers (Fox et al., 1977; Nakashima et al., 1977). This example will help to get an insight into the importance of an initiator ...

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Epigenetic regulation of human cancer/testis antigen gene, HAGE, in chronic myeloid leukemia.

Epigenetic regulation of human cancer/testis antigen gene, HAGE, in chronic myeloid leukemia.

... Qrt-PCR for HAGE expression was performed with the LightCycler technology, using 1 µ L of cDNA in a 20 µ L reaction volume with 0.4 µ mol/L of each primer (for- ward: 5’-CCTTTCAATGTTATCCTGAG-3’; reverse: 5’- ...

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Approach to the diagnosis of drug hypersensitivity reactions: similarities and differences between Europe and North America

Approach to the diagnosis of drug hypersensitivity reactions: similarities and differences between Europe and North America

... The reference standard for drug tolerance is no evi- dence of reaction after a therapeutic dose of the impli- cated drug. For a suspected IgE-mediated IDHR at least 1–6 h of observation are necessary. For a ...

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