Quantitative real-time PCR
Selection of Reference Genes for Quantitative Real Time PCR (qPCR) Assays in Tissue from Human Ascending Aorta.
... Dilatation of the ascending aorta (AAD) is a prevalent aortopathy that occurs frequently associated with bicuspid aortic valve (BAV), the most common human congenital cardiac malformation. The molecular mechanisms ...
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PCR en tiempo real
... in real-time ...In PCR the fluorescence of these dyes increases with the amount of double stranded product formed, though not strictly in proportion because the dye fluores- cence depends on the ...
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Chilling dependent release of seed and bud dormancy in peach associates to common changes in gene expression
... for PCR reactions in a final volume of 20 ml. Quantitative real-time PCR was performed on a StepOne- Plus Real-Time PCR System (Life Technologies, Carlsbad, CA, ...
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TítuloEvaluation of Plakophilin 3 mRNA as a biomarker for detection of circulating tumor cells in gastrointestinal cancer patients
... Using real-time quantitative PCR, we showed that PKP3 mRNA was overexpressed in colorectal tumors in comparison with matched normal colonic ...
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Real time PCR Application in dengue studies
... in quantitative PCR ...a real-time PCR carried out with SYBR Green I must include doing a dissociation curve by measuring the fluorescence emitted by the SYBR Green I along a ...
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Application of Molecular Diagnostic Techniques for Viral Testing.
... (PCR), real-time PCR or nucleic acid sequence-based amplification (NASBA) [3] for virus detection, genotyping and quantification have some advantages like high sensitivity and reproducibility, ...
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Identification of reference genes for quantitative RT-PCR in ascending aortic aneurysms.
... The expression levels of 32 commonly used candidate reference genes (Table 1) were determined using TaqMan human endog- enous control plates (Applied Biosystems). These plates were analyzed using StepOnePlus ...
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Weighted real time heuristic search
... to Real-Time Heuristic Search, a search approach that builds upon Heuristic ...in Real-Time Heuristic ...the Real-Time Heuristic Search ...of Real-Time Heuristic ...
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Multiplexing real time video services
... model: even the mean rate cannot be estimated beforehand. The study of this type of sources is therefore of limited practical interest. No reasonable trac contract can be maintained during the communication, and ...
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Estandarización de técnicas moleculares por PCR convencional y PCR en tiempo real para el diagnóstico de rubéola en el INS
... de PCR convencional y PCR en tiempo real basado en sondas TaqMan para detectar el gen E1 en aislamientos del VR, como alternativa o complemento de los métodos serológicos de diagnóstico utilizados en ...
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Bordetellas, más que solo pertussis: Genética, genómica, evolución y nuevos patǵenos para la especie humana
... Evaluation of real-time PCR for detection of and discrimination between Bordetella pertussis, Bordetella par- apertussis, and Bordetella holmesii for clinical diagnosis.. Friedman LE, M[r] ...
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Real-time speckle image processing
... in real time makes it possible to develop important practical applications of commercial, biological and technological ...with real-time constraints with a maximum throughput of about thousand ...
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Evaluación de distintas variedades de cucurbita para resistencia al virus ToLCNDV
... Almudena Alonso Rivas 49 las plantas inoculadas a los 30 dpi, demuestra que las plantas asintomáticas de estas accesiones han sido capaces de restringir la multiplicación y movimiento del virus en la planta, por lo que ...
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Anàlisi de l'expressió i dels mecanismes d'acció del receptor d'estrògens beta en el càncer de pròstata
... Per tal d’obtenir DNA plasmídic es van realitzar minipreps a partir de cultius crescuts tota la nit en presència de l’antibiòtic requerit. El DNA utilitzat per a clonatges in vitro, digestions i reaccions de PCR ...
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An embedded real-time co-processor for control applications
... Section 2 introduces the main concepts on digital control. Section 3 describes a typical implementation of a real-time operating system. In section 4 we present the effects that a scheduling mechanism ...
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On real-time partitioned multicore systems
... We have analysed the implications of such architecture for developing real-time systems using Ada and the Ravenscar profile, and have proposed an implementation scheme based on the assu[r] ...
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Ada real-time services and virtualization
... on real-time programs using the services de- fined in the Ada real-time annex [4, annex ...Ada real-time ser- vices are summarized in section 3, and the implications of ...
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Mecanismos de regulación de AmpC en Pseudomonas aeruginosa: evaluación de nuevas dianas para evitar la resistencia a β lactámicos
... Para ello, previamente se elaboraron células competentes de XL1 blue de la siguiente manera: se incubó el cultivo de dicha cepa hasta la fase exponencial (con aproximadamente 1x10 8 células/mL) y tras sucesivas ...
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Infección por Mycoplasma suis en el cerdo . Una revisión bibliográfica
... Abstract: Mycoplasma suis is a microorganism epi-cellular not cultivable, sensitive to tetracy- cline, which adheres and deform the surface of erythrocyte causing cell lysis. Acute infections in pigs are clinically ...
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Reacción en cadena de la polimerasa (PCR) a tiempo real
... En la tabla 2 se describen los equipos para llevar a cabo la PCR a tiempo real y sus fabricantes. Están compuestos por un termociclador y por un lector de fluorescencia y dise- ñados para poder efectuar la ...
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