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Human Choroid Plexus Papilloma Cells Efficiently Transport Glucose and Vitamin C

Human Choroid Plexus Papilloma Cells Efficiently Transport Glucose and Vitamin C

cultures of HCPP cells (the bystander cells) (Nualart et al. 2003). As depicted in Fig. 6a, adherent HCPP cell mono- layers can be easily separated from human neutrophils that grow in suspension, permitting the determination of the content of vitamin C (AA) in each cell type separately (Fig. 6a). As expected, uptake of AA in untreated HCPP cells incubated alone was observed because of the presence of the AA transporter, SVCT2, in these cells (Fig. 6b, white circles). Treatment of HCPP cells with PMA (Fig. 6b, black circles) or co-culture with non-activated human neutrophils (Fig. 6b, white triangles) did not alter AA uptake. However, HCPP cells incubated in the presence of AA and co-cultured with PMA-activated human neutrophils showed a consider- able increase in vitamin C uptake (Fig. 6b, black triangles). HCPP cells contained more than 4 nmol of vitamin C per million cells, an amount 15-fold higher than that accumulated by PMA-treated HCPP cells incubated alone (compare black circles with black triangles in panel b). This uptake of vitamin C by stimulated HCPP cells (HCPP cells in presence of AA and PMA-activated human neutrophils) was com- pletely inhibited by cytochalasin B but not by cytochalasin E, confirming the participation of the glucose transporter in this process (Fig. 6c). HCPP cells did not produce superoxide
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Does vitamin C prevent the common cold?

Does vitamin C prevent the common cold?

50. Dick EC, Mink KA, Olander D, Schult PA, Jennings LC, Inhorn SL. Amelioration of rhinovirus type 16 (RV 16) colds in ascorbic acid sup- plemented volunteers [abstract]. 30th ICAAC Proceedings. 1990. 51. Scheunert VA. Adult requirements for vitamin C [Der Tagesbedarf des

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Títuloβ carotene, vitamin C and vitamin E content of the marine microalga Dunaliella tertiolecta cultured with different nitrogen sources

Títuloβ carotene, vitamin C and vitamin E content of the marine microalga Dunaliella tertiolecta cultured with different nitrogen sources

Variations in the β-carotene, vitamin C and vitamin E content of D. tertiolecta have been shown to result from the nitrogen source used in the culture medium. Differences of 101%, 38% and 69% have been found in β-carotene, ascorbic acid and tocopherol content in mg/g of dry matter, respectively, and differences of 147%, 63% and 37% occurred in β-carotene, vitamin C and E concentrations in mg/litre of culture, respectively. Considering the β-carotene, vitamin C and vitamin E content in mg/g of chlorophyll a, maximum variations occurred in β-carotene content, with differences of 145% among the different nitrogen sources. Maximum β-carotene and vitamin C values were found in urea cultures, whereas urea cultures showed the minimum values for vitamin E.
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Vitamin C improves the apparent absorption of levothyroxine in a subset of patients receiving this hormone for primary hypothyroidism

Vitamin C improves the apparent absorption of levothyroxine in a subset of patients receiving this hormone for primary hypothyroidism

In conclusion, our study demonstrated that changing the vehicle for ingestion of LT4 from plain water to the same volume of plain water with 1 g vitamin C improves the apparent absorption of LT4 –as it was evaluated indirectly by reduction in TSH level- in a subset of patients receiving this hormone for primary hypothyroidism who do not reach the target TSH level with the usual dose/weight of LT4. The important practical consequence of our finding is that a trial of coadministration of LT4 with vitamin C to enhance LT4 absorption should be considered in patients requiring high doses of LT4 with no obvious cause of LT4 malabsorption. This pilot study needs to be confirmed in a larger randomized, controlled trial.
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Isolation and molecular cloning of genes from Myrciaria dubia “camu-camu” with potential use for biotechnological production of vitamin C

Isolation and molecular cloning of genes from Myrciaria dubia “camu-camu” with potential use for biotechnological production of vitamin C

Do Nascimento, J.R., Higuchi, B.K., Gómez, M.L., Oshiro, R.A., Lajolo, F.M. 2005. L- Ascorbate biosynthesis in strawberries: L- galactono-1,4-lactone dehydrogenase expression during fruit development and ripening. Postharvest Biol. Technol. 38:34-42. Fracassetti, D., Costa, C., Moulay, L., Tomás- Barberán, F.A. 2013. Ellagic acid derivatives, ellagitannins, proanthocyanidins and other phenolics, vitamin C and antioxidant capacity of two powder products from camu-camu fruit (Myrciaria dubia). Food Chem. 139:578-588. Frohman, M.A., Dush, M.K., Martin, G.R. 1988.
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Camu-camu - Myrciaria dubia (Kunth) McVaugh

Camu-camu - Myrciaria dubia (Kunth) McVaugh

Pioneering work on chemical and nutritional compositions of camu-camu fruits were performed in the early 1990s by Zapata and Dufour (1993). Further investigations have allowed us to understand, more exhaustively, the various chemi- cal and nutritional components that are contained within this native fruit plant of the Amazon. The chemical and nutri- tional compositions of camu-camu fruits are shown in Table 1. These fruits are composed of carbohydrate, proteins, lipids, ash, and several other chemical constituents. It is also a good source of essential amino acids and fatty acids, as well as vitamin C, B-complex vitamins, and vitamin A. This fruit, however, is very interesting especially for their exceptional high content of vitamin C (0.96 2.99 g/100 g), which is approximately 60 times higher than that of orange juice (Villachica, 1996; Alves et al., 2002; Castro et al., 2015; Castro et al., 2015; Zapata and Dufour, 1993; Rodrigues and Marx, 2006). Additionally, it contains several minerals such as potassium, phosphate, sulfate, calcium, magnesium, chloride, sodium, copper, cobalt, iron, and selenium.
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Effect of ultrasound treatment on bioactive compounds’ composition of soybean and red prickly pear used to develop a functional beverage

Effect of ultrasound treatment on bioactive compounds’ composition of soybean and red prickly pear used to develop a functional beverage

In terms of betalains, the mixed beverage presented a lower concentration than the theoretical value in most of the drinks. Possible degradation reactions could occur during the manufacture of the mixed drink that caused the observed loss. Herbach et al. (2006) described that the detrimental effects on betalain integrity are mainly caused by temperature, oxygen, and light. The highest concentrations of betalains were quantified in the mixed beverage made with the control and treated prickly pear stored at 24 h (Figure 5.17A). However, there were no significant differences between the mixed beverages made with the control and treated prickly pears stored at 0 h and 12 h. As well as betalains, the vitamin C in the mixed beverages exhibited lower content than the theoretical values, this may be due to the oxygen and light exposure during the production of the mixed beverage (Oyetade et al., 2012). The higher content of vitamin C was quantified in the mixed beverage made with processed prickly pear and stored at 24 h (Figure 5.17B). The beverages produced with the sonicated red prickly pears that were stored for 12 h showed a significant increase of phenolic compounds compared to the control. Nevertheless, the optimized beverages made with the red prickly pears that were stored for 0 and 24 h did not present significant changes in their phenolic concentration.
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Preparation and evaluation of appertized from snail Helix aspersa M.

Preparation and evaluation of appertized from snail Helix aspersa M.

fied by the presence of ingredients as kilol and citric acid in this treatment (Table 7). Kilol, which possesses antioxidant activity, helps maintain the organoleptic characteris- tics, conservation and/or stability of the food, does not alter the food nutritional value, protects from protein degradation, prevents oxidation of lipids, among others. This product is used in the food industry, since it is a seed extract (80%) and pulp (20%) of orange and composed of other natural elements, ascorbic acid (vitamin C), glycerides, tocopherols, amino acids and other non–toxic, being currently the most widely used preservative in the food indus- try for salmon in Chile (ISP, 1998). Mean- while citric acid in packed pork meat stabi- lizes the color; additionally, for the case of bacon and cured meats, it promotes the appearance of color and prevents black spots on shrimp (ISP, 1998).
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Quality attributes and functional compounds of Mexican plum (Spondias purpurea L.) fruit ecotypes

Quality attributes and functional compounds of Mexican plum (Spondias purpurea L.) fruit ecotypes

always higher in the epicarp than in the pulp. Similarly, Ahmed et al. [42] reported a higher antioxidant activity in the epi- carp than in the pulp of Lagenaria siceria fruits and they ex- plained that the abundance of phenols in the epicarp was the reason of this difference. The ecotype with the highest content of antioxidant activity present on either epicarp or pulp was ’Conservera de Tlaxmalac’ with 5.1 mM antioxidant capac- ity equivalent to concentration of ascorbic acid (ACECA) g −1 FW in the rind and 1.6 mM ACECA g −1 FW in the pulp. The ecotypes with the lowest content of antioxidant activity equiva- lents were ’Costilluda’ with 1.09 mM in the rind and ’Bolsuda’ with 0.34 mM ACECA g −1 FW in the pulp. On average, the an- tioxidant capacity that was measured in the ecotypes examined was 3.0 mM ACECA g −1 FW in the rind and 0.7 mM ACECA g − 1 FW in the pulp (table III). Floegel et al. [43] found antiox- idant activities (in equivalents of vitamin C) ranging from 6.8 to 521.0 mg 100 g −1 FW when they analyzed the 18 most con- sumed fruits in the United States; unfortunately, they did not indicate if the assays were conducted in the pulp or the whole fruit (peel and pulp). However, the values found in the present study are in the range reported by these authors (on average, 123 and 528 mg 100 g −1 FW equivalent to vitamin C for pulp and rind, respectively).
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Compuestos bioactivos y capacidad antioxidante en frutos nativos del Uruguay

Compuestos bioactivos y capacidad antioxidante en frutos nativos del Uruguay

Accessions of native fruits from ‘Arazá’ (Psidium cattleianum), ‘Guaviyú’ (Myrcianthes pungens) and ‘Guayabo del país’ (Acca sellowiana) were evaluated as part of a se- lection program to obtain commercial cultivars, with the aim to offer the growers new possibilities of local fruit crops and provide the consumers with food alter- natives with recognized nutritional and nutraceutical properties. The objective of this work was to evaluate the physicochemical quality, the total phenolic content, vitamin C and total anthocyanins, and the relationship with the total antioxidant activity by DPPH and ORAC for different fruit genotypes. The methodologies for the determination of bioactive compounds and antioxidant capacity were validated on a microvolume scale, following the principles of “green chemistry”. The content of bioactive compounds differed significantly depending on the species and accession (p  ≤  0,05). The results obtained confirmed the nutritional properties and potential antioxidant capacity of the native fruits. The effect of agronomic and environmen- tal variables on selected genotypes should be investigated to adjust commercial production technology in order to exploit the nutraceutical characteristics found. Keywords: native fruits, vitamin C, phenolic compounds, DPPH, ORAC.
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Simplified method for vitamin E determination in rat adipose tissue and mammary glands by high performance liquid chromatography / C  Barbas     [et al ]

Simplified method for vitamin E determination in rat adipose tissue and mammary glands by high performance liquid chromatography / C Barbas [et al ]

ples, without tedious pretreatment, only a small The following results were found in adipose and mammary gland tissues of virgin and pregnant rats 24 h after receiving one oral dose of vitamin E: vitamin E content in adipose tissue appeared very similar in virgin (33.35614.5 mg / g) and pregnant rats (35.9766.4 mg / g), values being in the range given by Bieri and Evarts [12] for tissues from untreated non pregnant Sprague Dawley rats, and higher than those found by Liu and Huang [14] in Long Evans rats. However, a statistically significant (Student test, p,0.05) higher vitamin E content was found in mammary gland from pregnant than virgin rats.
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Improving osseointegration of titanium implants by a novel bioactive coating with UV irradiated 7 dehydrocholesterol

Improving osseointegration of titanium implants by a novel bioactive coating with UV irradiated 7 dehydrocholesterol

identified suppressive effects of high dose vitamin D supplementation on bone formation (Yamaguchi and Weitzmann, 2012). However, the lower presence of blood observed in the interface after removal of VitD-LD coated implants suggested that this coating accelerates the formation of the coagulum and its further replacement with granulation tissue and osteoid. Indeed, the ALP activity results revealed a higher degree of matrix mineralization for VitD-LD coated implants. Furthermore, VitD-LD coated implants showed the most suitable balance in mRNA levels of markers involved in bone formation and bone resorption in the bone-implant interface. Thus, a trend to decrease COLL-1 whilst to increase BMP-2 and RUNX2 mRNA levels were found in VitD-LD samples, which then significantly increased OC levels. Also, lower TRAP mRNA levels were observed, in line with the inhibition of the osteoclastogenesis in vitro (Satué et al., 2014). The mRNA levels of cytokines expressed by monocytes and macrophages in the peri-implant bone tissue revealed that VitD-LD coated implants may trigger the most appropriate inflammatory response during healing. Finally, peri-implant bone tissue in contact with VitD-LD coated surfaces tended to up-regulate CYP27A1 and VDR mRNA levels, suggesting an increased vitamin D synthesis in the peri-implant bone tissue. Similarly, systemic administration of vitamin D in different animal models has resulted in the improvement of the implants osseointegration (Dvorak et al., 2012; Wu et al., 2013; Liu et al., 2014) but also the action of implants treated with vitamin D locally has been investigated in vivo. Thus, Cho et al. (Cho et al., 2011) demonstrated that PLGA/1,25(OH) 2 D 3 coating stimulated bone formation
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Pobreza y nutrición : tres diagnósticos sobre la niñez en Argentina

Pobreza y nutrición : tres diagnósticos sobre la niñez en Argentina

Dietary patterns established in childhood may significantly influence the probability of acquiring several chronic health conditions that can reduce welfare and hasten mortality (National Research Council, 1989). Hence, children have been identified as an important target group for national public health initiatives aimed at health promotion and disease prevention. It is easier to establish healthful habits during childhood than to attempt to change eating habits later in life. Thus, childhood offers an opportunity for nutrition education to influence the establishment of healthful lifelong eating patterns. In developing nutrition education programs for children, health professionals benefit from information about the diet quality of diverse groups of children. Knowledge of children's dietary patterns allows for the targeting of nutrition information to groups where nutrition intervention is needed to improve risk factor profiles. (Johnson et al., 2004) Micronutrients such as vitamin A, iron and various minerals and trace elements are not produced by the body and are required in only small quantities. Yet, micronutrient deficiencies can cause learning disabilities, impair work capacity, and have been associated with heightened morbidity and mortality- particularly among pre-school children and pregnant women. (World Bank, 1994)
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Enfermedad arterial perifrica silente en pacientes ingresados en el servicio de urgencia

Enfermedad arterial perifrica silente en pacientes ingresados en el servicio de urgencia

No es de extrañar que de forma similar a los estudios VITAMIN, Ankle Brachial Index Colaboration, CAREFUL y otros, la mayor asociación para EAP se encuentre con las variables de cardiopatía, insuficiencia renal, dislipide- mia, tabaquismo y obesidad, así como las causas de ingre- so de enfermedad vascular cerebral, síndrome isquémico coronario agudo y descontrol metabólico, patologías que comparten un mismo sustento fisiopatológico generador de aterotrombosis, es decir, un estado inflamatorio endotelial crónico inducido por incremento en la ingesta de macro- nutrientes, obesidad, tabaquismo y tensión psicológica. 1-5,23
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Estudio de la variabilidad del gen crabp2 en el metabolismo lipídico y de la influencia del ácido retinoico en el endotelio vascular

Estudio de la variabilidad del gen crabp2 en el metabolismo lipídico y de la influencia del ácido retinoico en el endotelio vascular

Background: The cellular retinoic acid-binding protein II (CRABP-II), together with nuclear receptors such as the retinoid X receptor (RXR) and retinoic acid recep- tor (RAR), is involved in the transcriptional regulation of genes that control lipid metabolism via the retinoid signaling pathway and, as such, may be associated with disorders of lipid metabolism. Interestingly, the gene for CRABP-II is located on chromosome 1q21–23, which is a region that has been linked with disorders such as familial combined hyperlipidemia (FCHL), type 2 diabetes mellitus, and partial lipodys- trophy, all of which are characterized by dyslipidemia. Methods: We investigated the hypothesis that the CRABP2 gene is involved in the regulation of lipid metabolism. Using the promoter –394T)C polymor- phism of the CRABP2 gene, we performed association studies in three different cohorts: 299 healthy males, 182 HIV-infected patients and 151 patients with famil- ial hypercholesterolemia (FH). All cholesterol meas- urements were performed in the absence of any lipid-lowering agents. ANOVA was performed on data adjusted for age, body mass index (BMI), gender, and use of protease inhibitors.
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Vitamin D, precocious acute myocardial infarction, and exceptional longevity

Vitamin D, precocious acute myocardial infarction, and exceptional longevity

Blood samples were obtained under fasting consitions by venipuncture in an antecubital vein and collected in plain tubes. After clotting, tubes were centrifuged and sera were kept at -80 ºC until assayed. We measured serum 25(OH)D using the ARCHITECT 25-OH vitamin D chemiluminescent microparticle immunoassay (Abbott Diagnostics, Wiesbaden, Germany) [17]. Parathyroid hormone (PTH) was assayed with a chemiluminescent assay (PTH LIAISON N-TACT; DiaSorin, Saluggia, Italy). Serum calcium (Ca 2+ ) and phosphorus (P) levels were measured using a BM-Hitachi 747-200 autoanalyzer (Hitachi-Roche, Tokyo, Japan). Comparisons of biochemical parameters between the three subject groups were performed using ANOVA or Kruskal-Wallis tests, as appropriate. Serum concentrations of 25(OH)D were also dichotomized into 25(OH)D < 20 or 20 ng/mL, respectively, because this value has been previously shown to be a reliable cutoff for identifying subjects with greater disease risk [18]. Multivariate logistic regression analysis was performed to test the association between serum 25(OH)D < 20 ng/mL and EL and precocious AMI after adjustment for sex and CVD risk factors. error was set at 0.05 (two-tailed).
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Nivel de vitamina D y factores de riesgo cardiometabólicos en una población Pediátrica

Nivel de vitamina D y factores de riesgo cardiometabólicos en una población Pediátrica

: We performed an observational, descriptive study with an analytical component in 209 patients in whom we measured serum levels of 25-hydroxy-vitamin D (25 [OH] D) and evaluated their cardiovascular risk factors according to the criteria of the amended Adult Treatment Panel III (ATP III). Each group was compared using Student's t-test. A value of p <0.05 for two-tailed tests was considered significant. The average value of 25 (OH) D was 34,9ng / mL. The mean levels of 25 (OH) D was lower in the subgroup of patients with cardiovascular risk factors, namely: abdominal obesity, high blood pressure, elevated fasting blood glucose, low HDL- cholesterol levels and high triglycerides, however this difference was not statistically significant.
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Diferencias de C y C++.pdf

Diferencias de C y C++.pdf

C++ es un lenguaje libre estandarizado por ISO MUY GRANDE, que admite: Programación estructurada (la de toda la vida), la totalidad de la POO (objetos, herencia simple, herencia múltiple, polimorfismo, upcasting, downcasting, RTTI, interfaces, clases abstractas, clases amigas, operadores, sobrecarga... y mil cosas más avanzadas), programación genérica (plantillas, STL, conceptos de contenedores, estructuras de datos genéricas, <b>metaprogramación</b>... es una programación que no dispone ningún otro lenguaje mayoritario por el momento)... y otras características más avanzadas como los punteros inteligentes, programación lambda, programación "física"... También se usa como programación por eventos (MFC, Qt, Gtk y otras GUIs). Se usa para cualquier cosa, desde sistemas operativos hasta los juegos 3D de última generación pasando por servidores, pasando por las típicas aplicaciones de escritorio o un Office o OpenOffice, un reproductor WinAMP y Windows Media Player, un explorador web (Internet Explorer, Mozilla, Firefox...), un cliente eMule, un cliente Torrent.
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Effect of different doses of vitamin E on the incidence of malformations in pregnant diabetic rats / C  Barbas     [et al ]

Effect of different doses of vitamin E on the incidence of malformations in pregnant diabetic rats / C Barbas [et al ]

rats were used. Since it is a fact that the teratogenic effects of diabetes vary among different strains of rats [16], inter- species differences in the doses of vitamin E needed to obtain maximal benefits for the reversion of malforma- tions and reabsorptions caused by diabetes could be expected. In the present study 500-mg doses of vitamin E not only did not improve the malformation rate, but sig- nificantly increased the rate of embryo reabsorptions, sug- gesting that in the pregnant rat having diabetes high doses of vitamin E may be harmful. This effect has not been demonstrated before, because the vitamin E doses tested previously [6, 7] were always below the 500 mg used in the present study. Nevertheless, Siman and Eriksson [7] al- ready found a slight tendency toward an increasing rate of reabsorptions when high doses of vitamin E were admin- istered to nondiabetic rats.
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Consumo de energa y nutrimentos en mujeres mexicanas de entre 12 a 49 aos de edad: anlisis de la Encuesta Nacional de Nutricin 1999

Consumo de energa y nutrimentos en mujeres mexicanas de entre 12 a 49 aos de edad: anlisis de la Encuesta Nacional de Nutricin 1999

utilizando modelos de regresión lineal para encuestas complejas del logaritmo de la ingesta y la adecuación, ajus- tando para comparaciones múltiples con la prueba de Bon- ferroni. Resultados. La media nacional de ingesta de energía fue de 1 471 kcal. El riesgo de inadecuación (prevalencia de adecuación <50%) fue vitamina A: 38.3%, vitamina C: 45.5% y folato: 34.3%. La ingesta de carbohidratos, folato, hierro y calcio fue significativamente más alta en áreas rurales que en las urbanas. El riesgo de inadecuación fue mayor en mu- jeres del nivel socioeconómico más bajo para todos los nutrimentos con excepción de carbohidratos y calcio. Las adecuaciones de macro-nutrimentos fueron significativa- mente mayores en mujeres no-obesas. Conclusiones. Las diferencias entre las regiones, áreas del país y terciles de nivel socioeconómico reflejan una disponibilidad creciente de alimentos densamente calóricos de bajo costo en gru- pos marginales. Sin embargo, la energía total, colesterol y la
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