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Essential Role of a Trypanosome U4-Specific Sm Core Protein in Small Nuclear Ribonucleoprotein Assembly and Splicing
... for splicing defects after RNAi knockdown of SSm4 ...trans splicing, a putative RNA helicase gene, and the poly(A) polymerase (PAP) for both trans and cis splic- ...a specific component of the ... See full document
12
SMN Deficiency in SMA: Splicing Gone Awry
... is essential for the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs) (11, 14, 17-18, 20-23, ...two small nuclear RNAs (snRNAs), several proteins ... See full document
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Special Sm Core Complex Functions in Assembly of the U2 Small Nuclear Ribonucleoprotein of Trypanosoma brucei
... the trypanosome U2B ⬙ differs in its domain structure from its mammalian counterpart, we tested for this protein- protein interaction in ...the trypanosome orthologue to U2A ⬘ is called U2-40K ... See full document
5
Original Article Down-regulation of PTEN by HCV core protein through activating nuclear factor-κB
... HCV core mRNA was detected by regular RT-PCR using the following primers: for- ward: 5’-CGGGATCCATGAGCACGAATCCTAAACC- TCAA-3’, reverse: 5’-GGAATTCTTAGGCTGAAGC- ... See full document
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Distinctive immune response patterns of human and murine autoimmune sera to U1 small nuclear ribonucleoprotein C protein
... and U4-6 snRNPs (3–5), and are highly specific for the diagnosis of SLE (1, ...are specific for the unique proteins of U1 snRNPs (U1-70K, U1-A, and U1-C) (3, 5), although reactivity with a B 9 /B ... See full document
8
Anti A2/RA33 autoantibodies are directed to the RNA binding region of the A2 protein of the heterogeneous nuclear ribonucleoprotein complex Differential epitope recognition in rheumatoid arthritis, systemic lupus erythematosus, and mixed connective tissue disease
... The nuclear protein hnRNP-A2 (the RA33 antigen) belongs to the group of spliceosome-associated autoantigens (23). However, in contrast to the spliceosomal snRNPs, it is targeted not only by patients with ... See full document
24
Cellular Gene Expression Survey of Vaccinia Virus Infection of Human HeLa Cells
... and protein levels. The increase in WASP protein required viral DNA replication, shown by infecting cells in the presence of adenosine arabinoside, an inhibitor of VV DNA ...WASP protein level ... See full document
12
Assembly of avian reticuloendotheliosis virus: association of the core precursor polypeptide with the intracellular ribonucleoprotein complex.
... The absence of virion core polypeptides associated with the intracellular RNP complex suggests that the cleavage of Pr63 into the mature proteins takes place during or shortly after the [r] ... See full document
235
Small Capsid Protein pORF65 Is Essential for Assembly of Kaposi's Sarcoma-Associated Herpesvirus Capsids
... capsid protein, two triplex proteins, and the small capsid ...scaffold protein and the protease occupy the internal space. The assembly of KSHV capsids is thought to occur in a manner similar ... See full document
290
Analysis of a structured intronic region of the LMP2 pre mRNA from EBV reveals associations with human regulatory proteins and nuclear actin
... EBV (Epstein–Barr virus) is a ubiquitous human herpes virus that infects ~ 95% of adults [1]. EBV is implicated in various cancers [2–4] and autoimmune diseases [5]. Host-virus interactions are crucial to infection and ... See full document
12
Epstein-Barr Virus SM Protein Utilizes Cellular Splicing Factor SRp20 To Mediate Alternative Splicing
... transcripts. SM inhibits the expression of spliced cellular targets in trans- fection assays ...that SM inhib- its expression of the spliced immediate-early EBV transactiva- tor Rta ...of SM on ... See full document
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Host Factors in Enterovirus 71 Replication
... a specific interaction between EV71 2C and the RHDs of all four reticulon ...and specific interaction with the EV71 2C ...2C protein showed that isoleucine 25 is critical for the interaction of EV71 ... See full document
194
The Yeast Splicing Factor Prp40p Contains Functional Leucine-Rich Nuclear Export Signals That Are Essential for Splicing
... the essential U1 snRNP protein Prp40p, we performed a synthetic lethal screen in Saccharomyces ...leucine-rich nuclear export signal (NES) se- quences that fit the consensus sequence recognized by ... See full document
113
hnRNP C1/C2 and Pur-beta proteins mediate induction of senescence by oligonucleotides homologous to the telomere overhang
... (minimum essential medium) (Thermo Fisher Scientific, Pittsburg, PA, USA; Cat No 10-010-CV), supple- mented with 10% (volume/volume [v/v]) fetal bovine serum (FBS) (Atlanta Biologicals, Lawrenceville, GA, USA) and ... See full document
10
The Human Herpesvirus 8 Homolog of Epstein-Barr Virus SM Protein (KS-SM) Is a Posttranscriptional Activator of Gene Expression
... The newly discovered human herpesvirus, Kaposi’s sarcoma (KS)-associated herpesvirus or human herpesvirus 8 (HHV8), is causally associated with KS, primary effusion lymphoma, and multicentric Castleman’s disease (for a ... See full document
5
The 38K-Mediated Specific Dephosphorylation of the Viral Core Protein P6.9 Plays an Important Role in the Nucleocapsid Assembly of Autographa californica Multiple Nucleopolyhedrovirus
... a nuclear localization signal by prediction, it localized to the nuclei of transfected cells (53) and localized mainly to the center of the nucleus in the late phase during virus infection ...inner nuclear ... See full document
140
Genetic Evidence of an Essential Role for Cytomegalovirus Small Capsid Protein in Viral Growth
... capsid protein (UL86, 154 kDa), the minor capsid protein (UL85, 35 kDa), the minor capsid protein-binding protein (UL46, 33 kDa), and the small capsid protein (SCP, UL48/49), ... See full document
2
Comparative host protein interactions with HTLV 1 p30 and HTLV 2 p28: insights into difference in pathobiology of human retroviruses
... regulating splicing of mRNAs ...host protein, hnRNP F, which is not only involved in mRNA processing but also has the ability to bind DNA ...critical role in viral infection and ... See full document
12
Epstein-Barr Virus SM Protein Functions as an Alternative Splicing Factor
... Alternative splicing of RNA increases the coding potential of the genome and allows for additional regulatory control over gene ...alternative splicing remains to be defined but is likely to significantly ... See full document
117
Mechanisms of assembly and genome packaging in an RNA virus revealed by high-resolution cryo-EM
... capsid assembly and RNA packaging, understanding these roles has been difficult because the normal maturation of the RNA-filled capsid involves its cleavage and ... See full document
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