Top PDF Evaluación de la calidad del desempeño profesional docente y directivo del Colegio “César Vázquez Astudillo” de la parroquia Paccha, de la ciudad de Cuenca, provincia del Azuay durante el año 2011-2012
A mechanism of gene amplification driven by small DNA fragments.
... products of an endonuclease during 5 9-end resection or clipping of 39-DNA tails) [57,58], or they could form following reverse transcription of cellular RNAs into cDNAs ...is of note ...
J. Bras. Patol. Med. Lab. vol.41 número6
... (PETs). DNA can be extracted from PETs, but sometimes, it is unsuitable for molecular techniques as slow degradation of DNA occurs with ...aim of this study was to verify and discuss if ...
Restriction site extension PCR: a novel method for high-throughput characterization of tagged DNA fragments and genome walking.
... isolation of unknown genomic DNA fragments linked to DNA ...overhang of the endonuclease (KpnI, Nsi I, PstI, or SacI) restricted DNA fragments, extends the 39 end ...
J. Appl. Oral Sci. vol.12 número1
... One of the most revolutionary of these techniques regarding nucleic acid analysis is the polymerase chain reaction (PCR), which was first described in ...exponential amplification of specific ...
Comparison of LAMP and PCR for molecular mass screening of sand flies for Leishmania martiniquensis infection
... number of approximately 10,000 copies of the mkDNA gene in individual parasites (Simpson ...feature of PCR- mkDNA is the ability to differentiate ...applicability of PCR-mkDNA for ...
Biological, serological and molecular comparison between isolates of Cowpea severe mosaic virus
... samples of each virus isolate using the protocol developed by Rott & Jelkmann (2001), and used for synthesis of correspondent complementary DNA ...genomic fragments: PCR 1 and ...
Dissection of the beta-globin replication-initiation region reveals specific requirements for replicator elements during gene amplification.
... on gene expression. The replicator activity of this region has been thoroughly studied in the context of replication initiation at an ectopic chromosomal locus ...consists of two independent ...
An empirical approach for quantifying loop-mediated isothermal amplification (LAMP) using Escherichia coli as a model system.
... indicators of LAMP ...strains by using the two gene fragments responsible for vero-toxin (VT) production and tested against VT-producing (O157 and O45) and non-VT producing (DH5 alpha) ...
Metodologias moleculares para avaliar efeitos de aditivos e nutrientes na microflora intestinal das aves
... works by applying a small sample of DNA (or RNA) to an electrophoresis gel that contains a denaturing ...capable of inducing DNA to melt at various stages. As a result of ...
Fibroblast growth factor receptor 1 amplification in non-small cell lung cancer by quantitative real-time PCR.
... Collection of biospecimens and outcomes data complied with the Helsinki Declaration and was approved by the Wayne State University School of Medicine Institutional Review ...resection of their ...
Análise microbiológica de infecções endodônticas utilizando a associação das técnicas do Multiple Displacement Amplification (MDA) e da Hibridização DNA-DNA (Checkerboard)
... Mannheim) by vacuum and fixed onto the membrane by crosslinking using ultraviolet light (Stratalinker 1800, La Jolla, CA) followed by baking at 120˚C for 20 ...deposition of 28 different ...
DNA extraction and amplification conditions
... Most of the grapevine cultivars are ancient and were derived from different processes, such as differentiation from wild grapevines, spontaneous crosses between wild grapevines and cultivars or crosses between ...
DNA extraction, amplification and sequencing
... matrix of 1116 ...specimens of Singerocomus grouped into one distinct, well supported clade (BS = ...south of the Atlantic Forest clustered in a distinct well-supported ...
Clinics vol.61 número2
... Cryptosporidium DNA was amplified by PCR from the 29 stool samples using 4 different primer pairs that gener- ate a) 2 independent fragments from the C and N terminal domains of the COWP ...
Sequencing 16S rRNA gene fragments using the PacBio SMRT DNA sequencing system
... risk of assembly chimeras, it is unclear what the sequencing error rate is by this ...use of single molecule technologies that offer read lengths that are thousands of bases ...produced ...
Diagnosis of Streptococcus pneumoniae meningitis by polymerase chain reaction amplification of the gene for pneumolysin
... application of the PCR protocol presented in this study has been effective in the detection of ...surveillance of bacterial ...possibility of discrepant ...failure of PCR in these CSF ...
Trypanosoma cruzi Discret Typing Units (TcII and TcVI) in samples of patients from two municipalities of the Jequitinhonha Valley, MG, Brazil, using two molecular typing strategies
... (56/63) of Chagas disease, all born and living in the municipalities of Berilo (62 patients) and José Gonçalves de Minas (only one patient), distant 24 km, both of the Jequitinhonha Valley, MG, ...
GUS gene expression driven by a citrus promoter in transgenic tobacco and 'Valencia' sweet orange
... expression of genes activated by PAL promoters can be induced by biotic and abiotic factors, including wounding, ultraviolet light, pathogen infection or exposure to heavy metals salts (Kervinen et ...
Analysis of viral protein-2 encoding gene of avian encephalomyelitis virus from field specimens in Central Java region, Indonesia
... pairs of oligonucle- otide primer which were designed previously by Xie et ...samples of field specimens, a sample in lane 5 (sample code 499/4/12) is positive sample with clear DNA fragment ...
The comparison between effect of chemoradiation with weekly cisplatin with or without celecoxib in treatment of nasopharyngeal carcinoma: A phase III clinical trial
... Effects of nonselective cyclo oxygenase inhibition with low-dose ibuprofen on tumor growth, angiogenesis, metastasis, and survival in a mouse model of colorectal ...