... group of photosynthetic prokaryotes, with a large morphological diversity including unicellular, colonial and filamentous ...specialized in photosynthesis (vegetative cells) and in nitrogen fixation ...
... profiles of the gene encoding the large-subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (RLS), results from the in silico aggregation and experimental validation of the best number ...
... a suitablereference gene for expression normalization in ...involved in the process of protein translation, eIF-4a had the same performance as eEF-1a in most experimental ...
... one of the most common causes of shoulder ...failure of cuff healing, and reverse-transcription quantitative polymerase chain reaction (RT-qPCR) has become an effective method for such ...
... used in the qRT-PCR analyses are presented in Table ...Standard RT-PCR was performed for all the primer pairs and a single amplifica- tion product of the expected size for each gene was ...
... studies of Chinese jujube, ZjH3 was reported as the most suitable housekeeping gene for evaluating jujube fruit-bearing shoot development by semi-quantitative RT-PCR [3], whereas, its expression was ...
... validation ofreferencegenes has been carried out in several cucurbitaceous crops, including melons, cucumbers, and zucchi- ...nis. In cucumbers, EF1a, Fbox, CAC and TIP41 have been ...
... Referencegenes have to be validated and consistently expressed under various ...used referencegenes for expression analyses include GAPDH, ACTB, and HPRT (Blaha et ...These ...
... all referencegenes had a suitable performance and showed very high stability measurements in all experimental conditions ...best genes to be used in our forthcoming qPCR ...
... deformation of the glenohumeral ...understanding of capsule deformation, and reverse-transcription quantitative polymerase chain reaction (RT-qPCR) has become an effective method for such ...
... normalization of gene expression using an endogenous reference ...Ideal referencegenes should be sufficiently abundant and have stable expression across differ- ent tissues and cell lines ...
... component in determining the function of a given gene in molecular biology and such studies help us to understand the growth and development of different ...species. RT-qPCR is ...
... quantification of accurate gene expres- sion have an increasingly important role in studies aiming for the reliable examination of expression profiles gener- ated by high-throughput ...one of ...
... Referencegenes are commonly used for normalization of target gene expression during RT-qPCR ...housekeeping genes or referencegenes have been identified to be ...
... applicability of this candidate reference gene set on different experimental conditions, we performed a similar evaluation ofsuitablereferencegenesin acute phase ...
... Selectionof candidate referencegenes and mRNA transcript levels To ensure accurate analysis of gene expression, ten candidate referencegenes (Table 1) were investigated ...
... that, in dogs, the most stable control genes were ribosomal protein S5 in the liver, kidney, and mammary glands, beta 2-microglobulin (B2M ) in the left ventricle, and ribosomal protein L8 ...
... influence ofreferencegenes on the expression profiles of target genes, cells cultured in GM, IM and CM were harvested at day 7 followed by qPCR ...data of fold ...
... quantification of gene expression studies. Appropriate application ofRT-qPCR requires accurate normalization though the use ofreference ...single reference gene is ...
... os genes nodABC são transcritos em poucos minutos após a indução por flavonoides, por participarem da fase inicial do processo de ...que genes do operon nodABC possuem maior expressão no período anterior a ...