[PDF] Top 20 Handmade cloned bovine embryos, parthenogenesis and in vitro fertilization: a comparison

... The handmade cloning and parthenogenesis as a control leads to the possibility of studying biological processes such as cell reprogramming, epigenetics, embryo genome activation and pre- ... See full document

... the in vitro maturation (IVM) of ovine (Ovis aries) oocytes and the development of handmade cloned embryos was eval- ...maturation and reactive oxygen species (ROS) levels ... See full document

... 24h and hatching of 76% at 72h post thaw following a vitrification protocol including cryoprotection afforded by 5 min exposure to ethylene glycol at 27 o ...unselected embryos, and 60% of 15 ... See full document

... proteins in more than one step of the fertilization ...participate in the release of proteins from the acrosomal matrix, binding of acrosome-reacted sperm to and penetration of the ZP, ... See full document

... differences in the three culture media used, as eva- luated by the occurrence of the first cleavage divi- sion ...development in control medium was blocked at the two-cell stage (Figure 1A), and only ... See full document

... apices in order to achieve survival after ...solution and freezing in liquid ...sucrose and other sugars which allow cells to survive under conditions of hydric stress and at the same ... See full document

... ºC in oscillat- ing bath at 20-25 ...centrifuged and washed twice in cold RPMI 1640 cell culture ...cells in different microscope fields by triplicate ... See full document

... performed in general IVF population (6, 7), a similar trend was observed in the duration of stimulation and gonadotropin ...difference in the number of retrieved oocytes was less important ... See full document

... fracture and tor- sional preload on K3 and K3XF heat treated instru- ...immersed in an aqueous ...K3XF and K3 (n = 15 for each group) were used in an apparatus for folding in 3 ... See full document

... organic in all the variables evaluated, such as plant height which increased by ...elements in the nutrient solution were readily available [16], and favored the growth of plants in ... See full document

... [57]. In this study, bone marrow-derived MSCs were trans- planted into the articular cartilage defect and covered with autologous ...arthroscopic and histological grading score was better in ... See full document

... 1 and F2 formed several nodules of dense tissue (more extended in the F2 case), located near the fil- ters (Figures ...cartilagenous, and, even, osteoid. They showed weak alcianofilia (Figure 3) ... See full document

... than in the cultures of NAA / ...that in NAA / BA the explants produce meristemoids even in later stages of ...known, in other systems it has been reported that the ability of the explants to ... See full document

... divided in two equal parts, the fi rst was mixed in a proportion of 1:1 with AL27 fraction previously diluted to a ...concentration and incu- bated for 1 h at 37°C and the second (PI-3) was ... See full document

... to fertilization with P. Differences in biomass production did not generate differences in soil carbon content at the end of the ...evaluation. In spite ...conditions and that can be ... See full document

... Las relaciones entre residuos de rendimiento de soja para el año 2013 y el nivel nutricional medido como concentración de K en hoja en R3 también fueron diferentes entre tratamientos (Fi[r] ... See full document

... One of the experiments carried out for the purposes of this thesis (Experiment 1) was conducted at “La Chimenea” field station (40°03’N, 3°31’W, 550 m a.s.l.) between April 2015 and September 2015 (Fig. 3.2). La ... See full document

... Summary: Flowering plant sexuality in its strictest sense is limited to specific gametophytic cells — two male gametes (sperm cells) in the pollen (male gametophyte) and two female gamet[r] ... See full document

... spp. and Besnoitia besnoiti (20), which have not been studied in the country and should be ...prevalence in this study, was similar to that reported in different countries ...prevalent ... See full document

... En clonación, los ovocitos enucleados que han sido inyectados con una célula del animal donante deben ser activados químicamente para continuar su desarrollo ya que el núcleo de una célula somática no posee la capacidad ... See full document