... limited and can rarely regenerate a specific organ or tissue ...efficient and safe cell sources for regeneration of damaged tissues and organs or treatment for incurable ...embryonic stem ...
... stimulatory and inhibitory to cardiomy- ocyte differentiation depending on the application time during the differentiation process ...2A, and 2B), which coincided with the expression pattern of the FGF-10 ...
... germ cells (PGCs) are the embryonic precursors of the germ cell lineage, which are restricted to form only sperm and eggs following their specification from pluripotent epiblast ...in ...
... limit and therefore provide an inexhaustible source of cells for ...original cells reprogrammed; this precludes the need for complex procedures to create the genetic defect and avoids ...
... iPSCs and their derivatives should be immunologically tolerated by the ...embryonic andinducedpluripotentstemcells ...immunogenic cells could be retained after cell ...
... human induced pluripo- tent stem ...‘hit and run’ vector and subsequently infected with Adeno-Cre to remove the ...(H1), and reprogramming factor-free hiPSC lines(hiPSC-19 and ...
... (LIM1), and HESX Homeobox 1 (HESX1) and the secreted factor ...activated and initiates and maintains the integrity of the node and ...dorsal and ventral mesoderm, head and ...
... monolayers) and 44% (cardiospheres) on metabolic activity recovery was observed when ROCK inhibitor pretreatment was performed using this medium ...adhesion and cell aggregation by modulating gap junctions, ...
... REFs and rat neural precursors as starting cell types and relied on serum-containing medium, non- excisable moloney murine leukemia virus (MMLV)-based delivery (in combination with ES cell extracts), ...
... using stem cell-derived oligodendrocytes in graft-based remyelination therapy to restore action potential ...efficient and reliable cell source has not been introduced (for review, see ...regarding ...
... OCT-4/3 and NANOG have been used as epigenetic markers for iPSCs ...methylated stem cell-specific DMRs and significantly higher expression, and 17 genes with the hyper-methylated stem ...
... The pluripotentstemcells can potentially be used to counter a wide range of diseases, from diabetes to spinal cord injury, to childhood leukemia, to heart ...iPS cells are similar to human ...
... mal cells into osteoblasts, whereas Osx is believed to induce subsequent maturation of osteoblasts and inhibit chondrogenic ...specifically and exclusively expressed in all osteoblasts, showed ...
... these cells through ...35% cells were positive for EGFP expression ...survive and grow in serum-free media mTeSR1 and E8 which were used to maintains human ES or iPS cells [21,22], ...
... research, and has been widely applied as an animal model for studies in physiology, pharmacol- ogy, toxicology, nutrition, behavior, immunology, and neoplasia ...hypertension and diabetes has made ...
... Advancing pluripotentstem cell research to clinical applications requires adapting laboratory-scale cultivation methods to less conventional and current Good Manufacturing Practices- (cGMP- ) ...
... 4 cells/well in CnT-07 ...confluent. Cells were incubated with freshly sonicated fluorescent microspheres ...with cells for different time points (0, 2, 4 or 6 hour) at a final concentration of ...
... from inducedpluripotentstemcells (iPSCs) without contamination from unknown substances is crucial to their use in clinical ...progenitor cells, which retain hematopoietic and ...
... hiPSCs and donor cells with respect to hESCs further confirmed the proximity of hiPSCs to their corresponding cell of origin (Figure 5) as compared to other donor cell ...growth and proliferation, ...
... RE17 and RE19 from S7) carried fewer mutations, of which a subset was shared between them as well as with EPCs from the same ...line and these were not found in EPCs or the individual’s reference ...1D ...