Together, the chapters of this thesis provide an outlook for the investigation of transcription factors such as the GR. In particular, it seems essential to carefully quantify the processes involved. To this end, we must keep on developing novel analysis techniques. In addition, we need to couple localization in foci with binding and diffusion of the receptor. Looking at colocalization of the receptor with other nuclear factors will uncover the underlying biological mechanisms that dictate the distribution and dynamics of the GR. To this end microscopy tools, in particular single molecule approaches, remain a powerful tool. These tools should be combined with information on the conformation of DNA through tools such as HiC. Finally, the most information would be obtained through following a single receptor through multiple DNA binding and unbinding cycles. Therefore, increased
145 efforts to develop methods for high frame rate acquisition (milliseconds) over long time periods (hours) must be are required.
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