Argumentos en contra de la no fundamentación filosófica de los Derechos

Primers used for inverse PCR (section 3.1)

Gene Enzyme Primers

Pa-vbsA SalI vbsA3 and szdp75 or szdp76 and szdp79 Pa-vbsA BamHI vbsA3 and szdp79

Pa-vbsA ScaI vbsA3 and szdp79 Pa-vbsA EcoRI vbsA3 and szdp79

Pa-cypA BamHI szdp84 and szdp85 or szdp86 and szdp87 Pa-cypA PstI szdp84 and szdp88 Pa-cypA ScaI szdp84 and szdp87 Pa-cypA EcoRI szdp84 and szdp87

Degenerate primers (section 3.3)

Gene Primer name Primer nucleotide sequence (5’-3’) aflR szdp127 CAGGCGCGCYMBYRYWGYYT aflR szdp128 ACGTAGCCAYCCYSYGMRYR avnA aflG F1 GARTTYCCNAARGAYCCNATGCA avnA aflG F2 TGGTTYAAYTGGGCNACNTTYGA avnA aflG R1 GCNARCCARTCNGTRTCYTTCAT avnA aflG R2 GGYTTYTTRTCCCANARDATCCA verB vbrBF1 CACCARAARTAYGGNGAYACNGT verB vbrBF2 TACTTYAAYATGGCNATHTTYGA verB verBR3 TTATGAATNGCRTCNACCCANGG verB verBR4 TGGCCTGGNACNGCNGGRTACAT norA norAF1 GGCAACTTCATHGAYRYNGCNAA norA norAF2 GATGTNGCNAAYTTYTAYCARGG norA norAR3 GTAGTRAARTCCCACATRTGNACRTA norA norAR4 CCGATCACIGGRAA IACRTAIGG

Primers for sequencing

Name Sequence Gene Organism

MGPA1 CCTTCCACCATTCTTCTCTG cypA P. arachidicola MGPA2 TCGGGATGGATTCACTTGAC cypA P. arachidicola MGPA3 TCGATGGATGCGACTTCCTC cypA P. arachidicola MGPA4 CAATCAACATGGCCTGCTAC cypA P. arachidicola MGPA5 CGTCCAGATCCAGTGACAAG ver-1 P. arachidicola MGPA6 AGCTGGTTTAGCGGGTGCTC ver-1 P. arachidicola

MGPA7 GAAAGAGGTCGACGGCTGTG vbsA P. arachidicola MGPA8 CATCCCAGAATAGCCATGTG vbsA P. arachidicola MGPA9 ACTGCACCATCCGCATCATC cypA P. arachidicola MGPA10 AACGAGGAATGGACGCTCAG cypA P. arachidicola MGPA11 CAACGTAAGCTCCACCTCAC vbsA P. arachidicola MGPA12 CGCCAACTCCTGCACCAATG vbsA P. arachidicola MGPA13 TGGTATGGGTATGTGCAGAG vbsA P. arachidicola MGPA14 GGCAGGTAGGGTTGAGGTAG vbsA P. arachidicola MGPA15 GGACACTGATGGCCAACGAG cypA P. arachidicola MGPA16 TTGGCGGTGGACACAACAAG cypA P. arachidicola MGPA17 ACTGCCTTCCTGCAACAGTC cypA P. arachidicola MGPA18 ACCTCCTTTGGACCGATACG cypA P. arachidicola MGPA19 GGTCGTTCCATGTTGTGTTC moxA P. arachidicola MGPA20 AATAGCGTGCTGCGCATTCC moxA P. arachidicola MGPA21 ACACCACCAACCTACAAGTC vbsA P. arachidicola MGPA22 GATCTCCCTTCGAGTCTTTG pksA P. arachidicola MGPA23 CAGCTATCTCGCCGTCATGC pksA P. arachidicola MGPA24 AGCCACTTCTGCGTCGTACC ver-1 P. arachidicola MGPA25 GGGACATGTTGCTGGTTGTG vbsA P. arachidicola MGPA26 CACAAACCTCGGCTTCATAC vbsA P. arachidicola MGPA27 ATTGTGCCCGACACGACACC pksA P. arachidicola MGPA28 ACGTTCGTCCGGAGAAGTTG pksA P. arachidicola MGPA29 TAATTGGGCGCCTTTGATCG ver-1 P. arachidicola MGPA30 GCTGTCCTTCCACGGATCTG ver-1 P. arachidicola MGPA31 GGGCTCGATGCTCAATACTC vbsA P. arachidicola MGPA32 GCTGCGACTGCTTGTGGTTC vbsA P. arachidicola MGPA33 CTGTCTTTGCACCGCACGAG pksA P. arachidicola MGPA34 TCAACGTGGGCAGCGTTGTC pksA P. arachidicola MGPA35 TCGACAGCTTGAGCTCTATG pksA P. arachidicola MGPA36 ACGCATCTGTGCGGTATTTC ver-1 P. arachidicola MGPA37 ATAGCTCGCTACCAGGTTTG ver-1 P. arachidicola MGPA38 CGATCCTCGAACTCCCAAAC cypA P. arachidicola MGPA39 CCCATGAATGGTAGGGAAAG cypA P. arachidicola MGPA40 GGAACCAACAGCCAGTAGAC moxA P. arachidicola MGPA41 TATTCGCAGCTCGCTGTATC pksA P. arachidicola MGPA42 GCACCACAACTGGTAGATTC pksA P. arachidicola MGPA43 TGCGACATCCATCGACTATC ver-1 P. arachidicola MGPA44 TAGCGAGCGGACATCAAAGG ver-1 P. arachidicola MGPA45 CAAGGCTCTCGATGCATTTG vbsA P. arachidicola MGPA46 TGGTGCCGTGGTCATCCTAC vbsA P. arachidicola MGPA47 TGCTGTTGCTGTGGCTGTTG cypA P. arachidicola MGPA48 CGGTCAGCAGATGTAAGAAG cypA P. arachidicola MGPA49 ACTGGCCGACCTACTCTTTC vbsA P. arachidicola MGPA50 ACTCACACCCTCACCATGAC pksA P. arachidicola

MGPA51 CATCGAGATCGGCCCTAAGC pksA P. arachidicola MGPA52 AAGTGTCGTGGCCTCATAAC ver-1 P. arachidicola MGPA53 GGGTCGAACTTGAAGTACAC ver-1 P. arachidicola MGPA54 CAAAGCTTGCGCCAAGATCC cypA P. arachidicola MGPA55 CGAGGGTGCGGATGTAGATG vbsA P. arachidicola MGPA56 GACGTAGCCACGGCTGAAAG vbsA P. arachidicola MGPA57 TTCTCCCATGTCGTGACTTC ver-1 P. arachidicola MGPA58 GTACTGGCGAGCAACCAAAG ver-1 P. arachidicola MGPA59 TAGCTTCGCGCCTTGTCTGC ver-1 P. arachidicola MGPA60 GAAGCGGTTGTGGGTGTTAC ver-1 P. arachidicola MGPA61 AATTGAGCGGTGCCAGCATC ver-1 P. arachidicola MGPA62 GCGCCATTGATGGTCGTGTC pksA P. arachidicola MGPA63 AGCCGCTCCTCAAGGAGAAG pksA P. arachidicola MGPA64 CCATCTGTGGTGCTTCCTTC pksA P. arachidicola MGPA65 GTCTCCAGAATTGCCTTGAC pksA P. arachidicola MGPA66 TGAGCCTGTGGCTCCTGTTG cypA P. arachidicola MGPA67 TTGAGACCCGGAGTCCGATG cypA P. arachidicola MGPA68 TTGGCCGAACGGGTTGAAGG cypA P. arachidicola MGPA69 GTACTGTCTGCGCACAACTC cypA P. arachidicola MGPA70 TCCTCTCCTGCTCAGCTTTG ver-1 P. arachidicola MGPA71 CATGCCGATGGTGTAGATAG ver-1 P. arachidicola MGPA72 GTTGCGGTACTTGAACAGTC ver-1 P. arachidicola MGPA73 CAGCACGAACTCTTTCGATG ver-1 P. arachidicola MGPA74 AACCGTCTTCAACAGCTAGG ver-1 P. arachidicola MGPA75 TTGCGGCAAGGAGCATAGGC ver-1 P. arachidicola MGPA76 GCCGAAGAGGAACAACAAAG ver-1 P. arachidicola MGPA77 CCTGCGAGGCTAGTAGAAAG ver-1 P. arachidicola MGPA78 TAGCCTCGCAGGACCCAATG ver-1 P. arachidicola MGPA79 ATCGCCGAACACTTCCACTC ver-1 P. arachidicola MGPA80 TCCTCTTCGGCCTCCAATAC ver-1 P. arachidicola MGPA81 CGCAAGCCAAGCTACTTCTG ver-1 P. arachidicola MGPA82 GATCGAGACTCGATGGAATG ver-1 P. arachidicola

Appendix IV Nucleotide and amino acid sequence of Pa-pksA   M A P S N A T R V L V F G D Q T Y D F V 1 atggcaccctccaacgcaaccagagttctggtctttggggaccagacctacgacttcgtg 60 P R L R E L F Q V K D N P I L T A F L Q 61 cctaggttgcgagaactgttccaggtcaaggacaacccgatcctgactgccttcctgcaa 120 Q S H Y V V 121 cagtcacattatgtggtgtaggtggttgccactgaccaagcatggaaccgttactgacgc 180 R A Q M I Q A L P P A E H K A A R T 181 tagctagtcgggcacaaatgatccaggctctgcctccagcggagcacaaggcagcccgaa 240 F D L A D M L K K Y V A G K L S P A F Q 241 ccttcgacttggcagacatgctgaagaagtatgtcgctggcaagttgagccctgccttcc 300 T A L S C I T Q L G V F M R E F H D F T 301 agaccgccctcagctgcattacacaactgggtgtcttcatgcgagaattccacgacttta 360 K P Y P R H D S S Y V L G I C T G S L A 361 ccaagccgtatccacgacacgatagcagctatgtgctgggtatttgcaccggctctctcg 420 A A A V S S S S S L S E L L P I A V Q T 421 ccgccgcagcagtcagctccagcagctcgttgtccgagctcctgcccatcgctgtccaaa 480 A L I A F R L G L C V T D M R D R L E S 481 cagccttgatcgcctttcgcctcggcctgtgcgtcacagacatgcgcgatcgtcttgaaa 540 A T D D R T E P W S V V L F D T D E Q T 541 gcgccacagacgaccgcacagagccttggtcggtagttctcttcgacacagacgagcaga 600 A T K A I K D F C T E N V L P K T K Q P 601 ccgctaccaaggccatcaaggacttctgcaccgagaacgtccttccaaagacgaagcagc 660 W I T S A S S K T I T I S G A P R V L K 661 cttggatcacttccgcctcgtcgaagaccattactatcagcggcgctcctcgcgtgttga 720 H L T Q E P A L K D K K T R Q I P I Y V 721 agcacttgacacaagagcctgcactcaaggacaagaagacccgacagatcccaatctacg 780 P A H N S A L F T P E D V K A I L E T T 781 ttccagcgcacaactcggcgctctttacacctgaagatgtcaaggcaattctggagacga 840 P V E V W S K F P T K I P F I S S V S G 841 ccccagtcgaagtctggagcaaattccccaccaagattcctttcatctccagcgtctctg 900 K L A W A N S Y L A V M Q L A L N Q C L 901 gcaagctggcctgggcaaacagctatctcgccgtcatgcagctggcgctcaaccaatgcc 960 L E P V G W S H V E T E F P R L L K S R 961 tcctggagcccgtcggctggagccacgtcgaaacagagttccctcgacttctgaagtctc 1020 G A H N V L I T P I T T S A D R A L S A 1021 gaggcgctcacaacgtcctcattaccccaatcacgacttccgccgatcgtgccttgtccg 1080 A L S S T I S N I E V E K P S T A E T V 1081 ctgctctcagctcgaccatctccaacatcgaagtggagaagccttctacagctgagactg 1140 V H R P G E G K S K L A I V S M S G R F 1141 tcgtccaccgtcctggcgaagggaagagcaaactcgctatcgtctccatgtccggccgct 1200 P D S Q S T Q A F W D L L Y K G L D V V 119