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Las células que expresan alelos normales se aíslan y

In document Biologia básica (página 173-194)

Terapia génica

3. Las células que expresan alelos normales se aíslan y

For the calculation of the feed demand, animal numbers were used as delivered by the ICOPP partners via the ICOPP questionnaire. The data on organic animals were delivered, however, in several units and indicators (animals slaughtered, places, counted number at a specific date, heads per year, etc.); they were therefore transformed to harmonized units/indicators as shown in Table 5.

Table 5: Base for the harmonisation of indicators and units as used for the calculation of the de- mand of feed, crude protein, lysine, methionine and cysteine

Species  Indicator/Unit  Calculation

Pigs     

Breeding 

sows 

Litter per year 

(No./year) 

If not indicated otherwise in the questionnaire, we calculated with an 

average of 2 litters per sow and year.  Fattening 

pigs 

Fattening pigs slaugh‐

tered per year 

(No./year) 

For most countries the number of slaughtered animals was reported in 

the questionnaire. 

For both Austria and the UK, the number of places was reported. This 

was converted into slaughtered pigs, using the following formulas: Aus‐

tria: turnaround of 2.4 fattening pigs per place; UK: turnaround of 2.8 

fattening pigs per place.   Gilts  Raised gilts per year 

(No./year) 

If the number of raised gilts was not indicated in the questionnaire their 

number was calculated using the annual replacement rate for breeding 

sows. On average, across the ICOPP countries, 30 to 50 percent of the 

breeding sows are replaced each year.   Weaners  Raised weaners per 

year (No./year) 

It was assumed that the number of weaners corresponds to 106% of the 

fattening pigs. We assumed that each fattening pig was also a weaner, 

and 6% were added for gilts, losses, etc.) 

Poultry     

Broilers  Broilers slaughtered 

per year (No./year) 

For most countries the number of slaughtered animals was shown in the 

questionnaire.  

For countries, which did not supply the number of slaughtered animals, 

we assumed a turnaround quote between 4.15 and 4.55 per ‘animal 

place’ or ‘counted animal heads at a specific date’ depending on the 

country. 

Laying hens  Laying hen places (No)  For most countries, the number of places was reported in the question‐

naire. 

In the case of the numbers provided by Eurostat, where the laying hen 

number includes the rearing pullets, we assumed that 70 percent of 

these laying hen places.  Rearing 

pullets 

Number of raised 

rearing pullets per 

year (No./year) 

Some countries reported the number of rearing pullets. 

Where only a total number of laying hens was reported, it was assumed 

that the number of rearing pullets raised annually corresponded to the 

number of laying hens.  

Cattle      

Dairy cows  Number of dairy cows 

(No.) 

No transformation needed

Suckler cows  Number of suckler 

cows in Livestock 

Units (LSU)  

One suckler cow corresponds to 0.8 livestock units.  

6.4.2 Calculation of concentrate feed, crude protein, and essential amino acid

demand

For livestock an average concentrate feed, crude protein, and amino acid demand per single unit (e.g. “one fattening pig”) was estimated. It should be noted that for certain units these de- mands had to be adjusted to the countries’ feeding practices:

Breeding sows

The concentrate feed, protein, and amino acid demand for breeding sows was determined using the feeding recommendations and nutritive value tables of the Swiss Agroscope Liebefeld Posieux (ALP 2004). These feeding recommendations of ALP were used for all countries, in- cluding, however, suggestions for modifications made by the ICOPP partners.

The 42 days of lactation period and 140.5 days of non-lactation period (empty/non pregnant and gestation) are included in the calculations.

Table 6: Basic assumptions for the demand of concentrate feed, crude protein, lysine and methio- nine and cysteine for breeding sows

Species  Unit  Feed DM [kg]  CP [kg ]  Lys [kg]  Met [kg]  Met + Cys [kg] 

Breeding sows  per litter  671  110  5.13  1.88  3.74 

Source: ALP 2004, modified by FiBL/ICOPP partners

Fattening pigs

The feed, protein, and amino acid demand for fattening pigs was determined using the feeding recommendations of the Swiss Agroscope Liebefeld Posieux (ALP 2004).

Table 7: Basic assumptions for the demand of concentrate feed, crude protein, lysine, methionine and cysteine for fattening pigs

Species  Country  Slaughter  weight  [kg]  Age at  slaughter  [Days]  Feed DM  [kg]  CP [kg ]  Lys [kg]  Met [kg]  Met +  Cys [kg] 

Fattening pigs  Austria  125  220 280 52 2.63  0.782  1.55

   Denmark 102  171 235 39 2.24  0.722  1.43    Finland     225 280 52 2.63  0.782  1.55    France  120  190 280 52 2.63  0.782  1.55    Germany 125  280 52 2.63  0.782  1.55    Lithuania 125  220 280 52 2.63  0.782  1.55    Nether‐ lands  117.5  200 280 52 2.63  0.782  1.55    Sweden     280 52 2.63  0.782  1.55    Switzer‐ land  125  220 280 52 2.63  0.782  1.55    United  Kingdom  100  182 235 39 2.24  0.722  1.43

These feeding recommendations and nutritive value tables of ALP were used for all countries, including, however, suggestions for modifications made by the ICOPP partners.

For Denmark and the United Kingdom the numbers (Table 7) were adjusted because of lower age and/or lower average weight of fattening pigs at slaughter.

Gilts

The feed, protein, and amino acid demand for gilts was determined using the feeding recom- mendations of the Swiss Agroscope Liebefeld Posieux (ALP 2004).

These feeding recommendations and nutritive value tables of ALP were used for all countries, including, however, suggestions for modifications made by the ICOPP partners.

Table 8: Basic assumptions for the demand concentrate feed, crude protein, lysine, methionine and cysteine for fattening pigs

Species  Unit  Feed DM [kg]  CP [kg ]  Lys [kg]  Met [kg]  Met + Cys [kg] 

Gilts  per head  254  40  2.33  0.75  1.49 

Source: ALP 2004, modified by FiBL/ICOPP partners

Weaners

The concentrate feed, crude protein, and amino acid demand for weaners was determined us- ing the feeding recommendations of the Swiss Agroscope Liebefeld Posieux (ALP 2004, p. 55, ALP-Fütterungsempfehlungen und Nährwerttabellen für Schweine“).

These feeding recommendations and nutritive value tables of ALP were used for all countries, including, however, suggestions for modifications made by the ICOPP partners.

It was assumed that a pig is a weaner for 84 days, and that in the first 5 to 6 weeks the main feed intake is milk from the sow, with a small amount of additional feed in the 6th week. The suckling period was therefore not considered in the calculation for the feed demand.

Table 9: Basic assumptions for the demand of concentrate feed, crude protein, lysine, methionine and cysteine for weaners

Species  Unit  Feed DM [kg]  CP [kg ]  Lys [kg]  Met [kg]  Met + Cys [kg] 

Weaners  per head  27.4  5.26  0.36  0.115  0.232 

Source: ALP 2004, modified by FiBL/ICOPP partners

Broilers

Demand was calculated according to Bellof and Schmidt (2005; “Broiler production with 100 percent organic feed is possible”) and for France according to Leroyer and Lubac (2009). As broilers in France are kept until much older, about 115 days instead of 56 to 80 days in most other European countries, the demand was adjusted upwards.

Table 10: Basic assumptions for the demand of concentrate feed, crude protein, lysine, methio- nine and cysteine for broilers

 Species  Unit     Feed DM 

[kg] 

CP [kg ]  Lys [kg]  Met [kg]  Met + Cys 

[kg] 

Broilers  Per slaugh‐

tered animal 

Other 

countries 

4.87  1.07  0.052  0.019  0.042 

      France  6.03  1.3  0.063  0.023  0.051 

Source: FiBL elaboration based on Bellof and Schmidt (2005) and Leroyer and Lubac (2009).

Laying hens

For the calculation of concentrate feed, protein, and amino acid, the Layer management guide of Lohmann was used. The same figures were used for all countries.

Table 11: Basic assumptions for the demand of concentrate feed, crude protein, lysine, methio- nine and cysteine for broilers

Species  Unit  Feed DM [kg]  CP [kg ]  Lys [kg]  Met [kg]  Met + Cys [kg] 

Laying hen  One place  41.86  6.923  0.301  0.142  0.265 

Source: FiBL elaboration based on Lohmann (no year)

 

Rearing pullets

For the calculation of concentrate feed, protein, and amino acid, the Layer management guide of Lohmann of used. The same figures were used for all countries.

Table 12: Basic assumptions for the demand of concentrate feed, crude protein, lysine, methio- nine and cysteine for rearing pullets

Species  Unit  Feed DM [kg]  CP [kg ]  Lys [kg]  Met [kg]  Met + Cys [kg] 

Rearing pullet  Per head  6.864  1.169  0.058  0.025  0.047 

Source: FiBL elaboration based on Lohmann (no year)

Dairy cows

Only the concentrate feed part (energy and protein feed) of the dairy cow diet was included for the calculation of the feed and protein demand of cattle. Amino acids were calculated in order to get a complete picture of amino acids use, even though they are not essential for dairy cows. As the percentage of concentrate feed varies from country to country, from only 10 percent in Switzerland to up to 40 percent in Sweden, the demand was adjusted for each country.

In order to calculate the demand for concentrate feed, the data of Wiesinger (2008) was applied, using the data for the 3rd and 4th lactation (7,000 kg milk per year), based on Wiesinger’s recommendations that concentrate feed (energy and protein feed) corresponds to

Table 13: Basic assumptions for the demand of concentrate feed for dairy cows in the ICOPP countries

Country   

Austria  For Austria, the basic calculation from Wiesinger (2008) was applied.   Denmark  About 30% compound feed, calculated from the base calculation. 

Finland   Total feed is about 6500 kg DM, 30% are cereals, and 5% protein feed, mainly 

rapeseed cake (in total about 35% feed).  

France  About 1040 kg DM concentrate feed, which is about 16% of total feed 

(6500kg DM) with 18% protein content.  

Germany  With farms similar to Austria in the south and high output farms in the north, 

20% concentrate feed was assumed.  

Lithuania  20% concentrate feed assumed.  

Netherlands  ~1370 kg concentrate feed. Main components are wheat 25%, barley 25%, 

and sunflower expeller cake 10%. The protein content varies between 140 

(B‐pellet) and 160 (A‐pellet) gram per kg.  

Sweden  Up to 40 % is concentrate feed; the calculation base from Finland was used.  

Switzerland  10% concentrate feed 

United Kingdom  About 1525 kg DM concentrate feed with about 18% protein content.  

Source: FiBL elaboration based information from the ICOPP partners

Table 14: Basic assumptions for the calculation of the demand of feed, crude protein, lysine, me- thionine and cysteine for dairy cows (only for concentrate feed)

Species  Country  Unit 

Concentrate 

feed DM 

[kg] 

CP [kg ]  Lys [kg]  Met [kg]  Met + Cys 

[kg] 

Dairy cows  Austria  Per head and year  883  112  3.737  1.83  4.423 

   Denmark    1765  223  7.475  3.66  8.846     Finland    2275  330  14.435  5.514  14.399     France    1042  187  8.47  2.87  6.6     Germany    1177  149  4.983  2.44  5.897     Lithuania    1177  149  4.983  2.44  5.897     Sweden    2275  332  14.476  5.535  14.461     Switzerland    588    75  2.492  1.22  2.949     United Kingdom    1525  274  12.696  9.736  5.897     Netherlands     1370  200  7.67  3.36  8.2 

In document Biologia básica (página 173-194)