1.7 Delimitación y limitaciones de la investigación
2.2.4 Cómo se evalúa un plan estratégico de enseñanza
Genetic diversity is necessary for survival of a specie hence there is need to expand the study to wild varieties of S. nigrum.
Germplasm collection strategies should be aimed at preserving the overall genetic diversity by expanding sampling to other parts of Kenya
Samples from Mauche are genetically separated from other population. There is therefore need for further studies to determine this uniqueness.
REFERENCES
Abdul, S. Muaharaf, H. Mosnazi, A. Hosneara, H. and Monirul, A. (2012). SSR marker-based molecular characterization and genetic diversity analysis of aromatic landreces of rice (Oryza sativa L). Journal of Bioscience-Biotechnology, 1, 107– 116.
Agarwal, M. Shrivastava, N. and Padh, H. (2008). Advances in molecular marker techniques and their application in plant science. Plant Cell Resources, 27, 617– 631.
Allen, S. and Meyer, S. (2002). Ecology and ecological genetics of seed dormancy in downy brome. Weed Science, 50, 41–247.
Anas, J. and Yoshinda, L. (2004). Genetic diversity among Japanese cultivated sorghum Assessed with Simple Sequence Repeats markers. Plant Production Science, 5, 242–247.
Anderson, A. (2006). Vegetable foods in the world. New Scientist, 6, 138–142.
Andrew, J. Christopher, G. Jacqueline, B. Gary, B. and David, E. (2004). Simple sequence repeat marker loci discovery using SSR primer. Bioinformatics, 20, 1475– 1476.
Angeline, V. Maryke, L. and Elizma, K. (2010). Microsatellite-based assessment of five Solanum nigrum complex species and their progeny. Soil and Plant Science, 60, 494–499.
Ashwani, K. Sagwal, S. and Rani, S. (2012). An updated review of molecular genetics, phytochemistry and pharmacology of black nightshades (Solanum nugrum). International Journal of Pharmaceatical Sciences, 3, 226–229.
Assia, G. Menashe, H. and Alexandar, P. (2007). Germination behaviour of Solanum nigrum seeds. Journal of Experimental Botany, 35, 588–598.
Barkley, N. Roose, M. Krueger, R. and Federic, C. (2005). Assessing genetic diversity and population structure of citrus germplasm collection utillising SSR. Theoretical and Applied Genetic, 112, 519–1531.
Benor, S. Zhang, M. Wang, Z. and Zhang, H, (2008). Assessment of genetic variation in tomato (S. lycopersicum ) inbred lines using SSR molecular markers. Journal of Genetics and Genomics, 6, 373–379.
Bithell, S. Mckenzie, B. and Hill, G. (2002). Germination requirements of laboratory stored seeds of black nightshades. Newzealand Plant Protection, 55, 222–227. Chiej, R. (2010). A practical reference guide to more than 500 herbs, medicinal plants,
American plant nutrition value and utilization of traditional leafy vegetables in Kenya. Encyclopedia of Medicinal Plants. 336-340.
Chowdhury, N. Ghosh, A. and Gaitam,R. (2008). Mosquito larvacidal activity of Solanum villosum extract against the dengue vector Stegomyia aegyptae. BMC Complementary and Alternative Medicine, 8, 10-18.
Chweya, S. (1997). Identification and nutritional importance of indigenous vegetables in Kenya. African Symposium on Horticultural Crops, 2, 142–169.
Crouch, J. and Otriz, R. (2004). Applied genomics in the improvement of crops grown in Africa. American Journal of Botany, 3, 489–496.
Datema, E. Mueller, A. and Buels, R. (2008). Comparative BAC end sequence analysis of tomato and potato reveals over representation of specific gene families in potato. Plant Botany, 8, 1186–1471.
Defelice, D. and Michael, S. (2003). The black nightshades Solanum nigrum L. Poison, poultice and pie. Weed Society of America, 17, 421–427.
Dehmer, J. and Hammer, K. (2004). Taxonomic status and geographical provenance of germplasm accessions in the Solanum nigrum L complex, AFLP data. Genet Resource Crop Evolution, 5, 551–558.
Dhellot, J. Matouba, R. Nziokou, J. and Dzondo, M. (2006). Extraction and nutritional properties of Solanum nigrum seed oil. Journal of Biotechnology, 5, 987–991.
Douglas, E. and Pamela, S. (2003). The role of phylogenetics in comparative genomics. Plant Physiology, 132, 1790–1800.
Doyle, J. and Doyle, L. (1990). Isolation of plant DNA from fresh tissue. Plant Tissue Culture and Molecular Markers, 12, 513–517.
Duke, S. and Ayensu, A. (1985). Medicinal plant in China. Medicinal Plants in the World, 1, 382–420.
Edward, C. and Charles, B. (1990). Crossing relationships among diploid species of Solanum nigrum complex in North America. American Journal of Botany, 66, 709– 716.
Elena, A. Miguel, E. and Roger, T. (2010). Genetic diversity and population structure in the tomato like nightshades – Solanum lycopersicoides and Solanum sitiens. Annals of Botany, 105, 535–554.
Farooq, S. and Azam, F. (2002). Molecular markers in plant breeding (ii) some pre- requisite for use. Pakistani Journal of Biological Sciences, 5, 1141–1147.
Francis, C. and Rong, C. (1999). Population Genetic Analysis (POPGENE ver. 1.31). Furton, A. (2004). Molecular Marker Learning Modules, 2, 5–191.
Ganapathy, K. Gomashe, S. Rakshit, S. Ambelkar, S. and Biradar, B. (2012). genetic diversity revealed utility of SSR markers in classifying parental lines and elite genotypes of sorghum. Australian Journal of Crop Science, 6, 1486-1493. Gous, M. Mohd, Y. Adan, B. Puteh, Harun, A. Nurul, I. and Mohammad, A.
(2013). A review of most markers and their application in rice breeding programs to improve Blast Disease Resistance. International Journal of Molecular Sciences, 14, 22499–22528.
Grieve, M. (1995). A modern herbal. Botanical .com, 2. Grieve, M. (2012). A modern Herbal. Botanical .com, 2.
Gupta,P. Rustgi, S. Sharma, S. Singh, R. and Kumar, N. (2003). Transferable EST- SSR markers for the study of polymorphism and genetis diversity in bread wheat. Molecular Genetics and Genomics, 270, 315–323.
Guterreiz, C. Joana, S. and Irene, A. (2014). Replication of plant genome. Molecular Biology, 2, 1–23.
Hammington, H. (1974). Edible Native Plants of Rocky Mountains.University of New Mexico, Albuquerque, New Mexico.
Heather, M. and Matthew, R (2012). Genotyping with molecular markers : Scoring a molecular marker on an agarose gel. Plant Breeding and Genomics, 3, 25–34.
Homar, R. Jose, S. Muruaga, M. and Vargas, V. (2011). Genetic diversity analysis of common beans based on molecular markers.Genetics and Molecular Biolology, 4, 595–605.
Ignacimuthu, M. and Sankara, S. (2006). Ethnobotanical lnvestigations among tribes in Mudurai India. Journal of Ethnobiology and Ethnomedicine, 2, 2–25.
Jacob, M. Van den Berg,R. Vleeshouwer, V. Mank, R. Sengers, M. Hoeskatra, R. and Vosman, B. (2003). AFLP analysis reveals a lack of phylogenetic structure within Solanum section Petote. BMC Evolutionary Biology, 8, 145.
Jagatheeswari, T. Bharathi, H. and Jahabar, A. (2013). Black nightshades (Solanum nigrum L.) An updated overview. International Journal of Pharmaceatical and Biological Archives, 4, 288–295.
Jansen,R. Averbeke, W. and Slabbert, R. (2007). African leafy vegetables in South Africa. Water, 33, 317–326.
Jennifer, M. and James, A. (1977). Black nightshades-Solanum nigrum L. and related species. Promoting conservation and use of underutilized and neglected crops. International Plant Genetic Resources Institute, Rome, Italy, 5–78.
Jin, L. Lu, Y. Xiao, P. Sun, M. Corrke, H. and Bao, J. (2010). Genetic diversity and population structure of a diverse set of rice germplasm for association mapping. Theoretical Application Genetics, 121, 471–487.
Jonah, P. Bello, L. and Midaus, S. (2011). Importance of molecular markers in plant breeding programmes. Global Journal of Science, 11,5-12.
Jones, C. Edwards, K. Winfield, F. and Sala,C. (1997). Reproducibility of RAPDs, AFLP and SSR markers in plants by a network of European labolatories. Molecular Breeding , 3, 381–390.
Joshi, S. Prabhakar, K. Janjekar, P. and Gupta,V. (2011). Molecular markers in plant genome analysis. American Journal of Botany, 6, 230–240.
Juma, C. (1989). Biological diversity and innovation : conserving and utilizing genetic resources in Kenya. Africa Centre for Technology Studies, Reseaech Series, Nairobi, Kenya,139.
Kantety, R. Rota. L. (2002). Data mining for simple sequence repeats in expressed sequence tags for barley, maize, sorghum and wheat. Plant Molecular Biology, 48, 501-510.
Karuri, H. Ateka, E. Amata, R. Nyande, A. Muigai, A. Mwasime, E. and Gichuki, S. (2010). Evaluating Diversity among Kenyan Sweet Potato Genotypes Using Morphological and SSR Markers. International Journal of Agriculture & Biology, 12, 33–38.
Katarina, B. (2003). Phenology and shadow response of Solanum physalifolium var nitidi-baccatum and Solanum nigrum ssp. nigrum. PhD, UPPSALA University 1– 20.
Keterere, R. and Dibungi, L. (2010). Herbal medicines for animal health. Ethnoveterinery Botanical Medicine.Taylor Francis Group. CRC Press. 450. Kim, S. French, W. Liu, L. and Sappington, T.( 2008). Utility of EST- derived SSR
as population genetic markers in beetle. Journal of Heredity, 99, 112–124.
Koshi, Y. and king, G (2006). Simple sequence repeats as advantageous molecular markers –mutations in evolution. Trends in Genetics, 22, 252–259.
Kumar, P. Gupta, V. Misra, D, and Pandey, K. (2009). Potential of molecular markers in plant biotechnology. Plant Omics Journal, 2, 141–162.
Lana, S. and Krystic, L. (2002). Variability of leaf anatomical characteristics of Solanum nigrum from different habitats. Natural Sciences, 102, 59–70.
Lawson, O. and Zhang, A. (2006). Distinct Patters of SSR distribution in Arabidopsis thaliana and rice genomes. Genome Biology, 7, 14-16.
Lexa, G. Simone, A. and Holger, B. (2015). Characterisation of the Solanum nigrum complex of Kenya by AFLP markers. International Journal of Agricultural Science and Technology, 3, 10–16.
Lian, C. Abdul, M. and Wadud, Q. (2006). An improved technique for isolating codominant microsatellite markers. Journal of Plant Resources, 119,.415–417. Lim, T. (2013). Edible medicinal and non medicinal Plants, Springer press, Newyork. Ma, H. Yin, Y. Guoz, F. Cheng, L. Zhang, L. and Zhong, M. (2011). Establishment
of DNA fingerprinting for liaojing series of japonica rice. Middle East Journal of Scientific Research, 8, 384–392.
Malik, C. Chita W. and Bharneet, K. (2009). Markers used in diversity analysis. Crop Breeding and Biotechnology, 65–107.
Mammadow, J. Rajat, W. and Ramesh, B. (2012). SNP markers and their impact on plant breeding. International Journal of Plant Genomics, 10, 1155–1220.
Manoko, M. Feron, R. and Van der Weerden, M. (2008). Genetic diversity of the African hexaploid species Solanum scabrum Mill and Solanum nigrum L Solanaceae. Genetic Resouces and Crop Evolution, 55, 409–418.
Manoko, M. (2007). A systematic study of African solanum nigrum L, Section Solanum (Solanaceae).Radbound University, Nijmegen.
Martin, G. and Roger, M. (2007). Microsatelite and SNP markers in wheat breeding, genomics. Assisted Crop Improvements, 2, 1–24.
Masneuf-Pomarede, C. Durrens, P. Lollier, M. and Aigle, D. (2007). Molecular typing of wine yeast strains saccharomysis bayanas var uvanium using microsatellite markers. Systematic and Applied Microbiology, 30, 75–82.
Matsuoka, Y. Mitchell, S. and Kresorich, S. (2002). Microsatellites in Zea- variability, patterns of mutations and use of evolutionary studies. Theoritical Applied Genetics, 104, 436–450.
Maundu, J. Kiminywe, M. and Ngugi, G. (2004). Nutritional and indigenous vegetables in urban and peri-urban agriculture in Kenya.
Mbagwu, F. and Nwachukwu, C. (2007). Comparative leaf epidermal studies on Solanum nigrum and Solanum macrocarpon. Nature and Science, 5, 4.
Mkabwa, L. Berg, R. and Mariani, C. (2008). Genetic diversity of African hexaphoid species. Genetic Resources and Crop Evolution, 55, 409–418.
Mohan, J. and Suresh, N. (1997). Genome mapping, molecular markers and marker assisted selection in selected crop plants. Molecular Breeding, 3, 87–103.
Mohy-Ud-Dint, K.and Ahmad, M. (2010). Chemotaxonomic value of alkaloids in Solanum nigrum complex. Pakistan Journal of Botany, 42, 653–660.
Morganite, M. Olivie, A. Bhagwat, G. and Krishna, M. (1993). PCR amplified microsatellite as markers in plant genetics. The Plant Journal, 3, 175–182.
Mueller, L. Solo, N. and Taylor, R. (2005). The Solanum genomic network. A comparative resource of Solanaceae biology and beyond. Plant Physiology. 38, 1310–1317.
Mutegi, E. Sagnard, F. Semagn, K. Due, M. Muraya, B. and Kanyenji, D. (2011). Genetic structure and relationship within and between cultivated and wild sorghum (Sorghum bicolor) in Kenya as revealed by microsatellites, Theoritical and Applied Genetics, 122, 989–1004.
Mwai, N. Onyango, J. and Abukusta, M. (2007). Taxonomic Identification and characterization of African Nightshades (Solanum nigrum L section Solanum). African Journal of food, Nutrition and Development, 7, 216-232.
Nandhini,T.Paramaguru, P. and Vajayakumar, A. (2014). Morphological characterization and genetic variability study on Makoi (Solanum nigrum L) genotypes. Plant Archives, 14, 35–49.
Nei, M . (1987). Molecular Evolutionary Genetics Analysis.Columbia University, New York.
Nekesa, P. and M.eso, B. (1993). Traditional African Vegetables in Kenya. Production Marketing and Utilization, Organic Matter Management Network, Nairobi, Kenya. Nzioka, J. (1994). A focus on indigenous vegetables, case study : Kalama location ,
machakos district. Utilization and management of plant genetic resources , National Museum of Kenya, Nairobi, Kenya.
Ojiewo, C. Mwai, G. Abukutsa, M. and Stephen, R. (2013). Exploiting the genetic diversity of vegetable African nightshades. Bioremediation, Biodiversity and Bioavailability, 7, 6–13.
Olet, E. Kare, A. and Manfred, H. (2011). Amplified fragment length polymorphism analysis of species of Solanum section solanaceae from Uganda. African Journal of Biotechnology, 10, 6387–6395.
Olet, M. (2004). Taxonomy of Solanum L section Solanum in Uganda PhD thesis. Agricultural University of Norway.
Onyango, M. (1993). Effects of plant diversity and harvesting frequency on the yield of vegetable quality of four variant of black nightshades-Solanum nigrum. African Crop Science Conference Proceedings. pp. 1237–1240.
Panaud, A. Weeden, F. and Muhammad, A. (1995). Isolation of plant DNA from fresh tissues. Focus on Agriculture, 12, 13–15.
Patricia, F. Elizabeth, A. and Paulo,C. (2011). Molecular characterization of potato cultivars using SSR markers. Horticultura Brasileira, 29, 542–547.
Peakall, R. and Smouse, P. (2010). GenAIEX 6.41: Genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes, 6, 288–295.
Péter, P. and Hyvönen, J. (2011). On the Origin of Solanum nigrum : Can networks help?. Molecular Biology Reports, 38, 1171–1185.
Philips, R. and Vasil, I. (2001). DNA based moleculer marker in plants. Advances in Cellular and Molecular Biology in Plants, 6, 514–534.
Poczai, P. Matyas, K. Taller, J. and Szobo, L. (2010). Study of the origin of the rarely cultivated edible Solanum species: Morphological and molecular data. Biologia Plantarum, 54, 543–546.
Rajeev, K, Varsey, K and Roberto, T. (2007). Genomics-Assiated crop improvements. Genomics Approaches and Platforms, 1–12.
Ramachandran, S. Beukeboom, W. Gerace, N. Carranza S. and Michiels, N. (1997). Isolation and characterization of microsatellite loci from Planarian Dugesia, Platyhelminthes. Molecular Ecology, 6, 389–391.
Ramya,J. Anjali, S. Sanjay, G. and Indira, P. (2011). Current Perspectives on Therapeutic Properties Solanum nigrum. Alternative Medicine Review, 16, 78–85. Reid, B. (1997). Famine foods of Chiu-Huang Pents’ao.,
Rogger, B. Ogg, A. and Schilling,.E. (1981). Characterisation of black nightshades (Solanum nigrum) and related species in the United States. American Journal, 29, 27–32.
Rudy, S. (1998). Notes on Solanum scabrum and related black nightshade species. Natural Resources Institute, University of Greenwich.
Stracke, G. and Hammer, K.( 2008). Genetic diversity in the collection of Solanum nigrum L. in the Gatersleben genebank. Institute of Plant Genetics and Crop Plant Research (IPK) Genebank Corrensstr. 306466 Gatersleben
Sarah, D. (2009). The systematic and genetics of tomatoes on the Galapagos Islands (Solanum, Solanaceae ) PhD thesis. University College London.
Sayed, A. Fereshteh, M. and Mehdi, R. (2012). Phenolic compounds and antioxidant activity from saffron (Crocus sativus L) petal. Journal of Agricultural Sciences, 4, 175–181.
Schippers, R. (2002). African indigenous vegetables, An overview of the cultivated species.Revised version on Natural Resource International Limited, 252.
Schmidt, D. Danny, K. Silvia, S. and Baldwin, T. (2004). Solanum nigrum: A model expression system and its tools. Molecular Biology. 13, 981–995.
Selko, R. and Toone, S. (2006). Microsatelite practical guide. Ecology Letters, 9, 615– 625.
Semagn, K. Bjornstad, A. and Ndjiondjop, M. (2006). An overview of molecular marker method in plants. American Journal of Botany, 5, 2540–2568.
Sergel, V. and Irina, S. (2009). Genetic structure in two African vegetables management of shade species. International Journal of Plant Breeding, 3, 81–85. Sheej, T. Senan, S. Kizhakayil, D. and Sasikumar, B. (2014). Methods for
development of microsatellite markers : An overview. Notucae Scientia Biologicae, 6, 1–13.
Shoeb, H. Sayed, M. and Khalifa, A. (1990). Some Egyptian plants with molluscicidal activity. Egyptian Journal of Bilhazia, 12, 35–43.
Silva, T. Batista, M. Camara, C. and Agra, M. (2005). Molluscicidal activity of some Brazilian Solanum species (Solanaceae) against Biomphalaria glabrata. Annals of Tropical Medicine and Parasitology, 9, 419–425.
Son, Y. Kim, J. Chung, Y. and Lee, J. (2003). Ripe fruits of Solanum nigrum L. inhibit cell growth and induces apoptosis in MCF-7cells. Food and Chemical Toxicology, 41, 1421–1428.
Stagel, A. Portis, E. Toppino, L. Rotino, G. and Lanteri, S. (2008). Gene based microsatellite development for mapping and phylogeny studies in the egg plant. BMC Genomics, 9, 357.
Sue, P. Grant, L. Bernard, R. Baun, M. (1997). Mordification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Molecular Biology, 15, 8–15.
Taab, A. (2009). Seed dormancy and germination in S. nigrum and S. physalifolium as influenced by Temperature conditions PhD thesis. Swedish University of Agricultural Sciences, Uppsala.
Timothy, J. and Kokwaro, J. (1991). Food plants of the Luo of Siaya District of Kenya. Journal of Economic Botany, 45, 103–113.
Tindall, H. (1977). African leafy vegetables in South Africa. Agricultural Research Council, 1, 317–332.
Varshey, R. Viktor, K. and Adreas, B. (2004). Molecular maps in cereals; methodology and progress in cereal genomics. Cereal Genomics, 10, 1–18.
Wang, M. Barkley, N. and Jenkins, T. (2009). Microsatellite markers in plants and insects. Part I: applications of biotechnology. Genes, Genomes and, Genomics, 3, 54–67.
Wang, D. Shi, J. Carlson, S. Chen, C. and Erpeding, J. (2003). A low cost high throughout polyacylamide gel electrophoresis system for genotyping with microsatellite DNA markers. Crop Science Society of America, 43, 1828–1832. Werner, D. and Petra, L. (2010). Loose leafed boxed collection in the 19th century.
The vegetable garden, Vilmorin-Adrieux and Cie, Paris, France
William, J. Rafalski, J. and Tingey, S. (1991). RAPD marker- A new technology for genetic mapping and plant breeding. AgBiotech News Inform, 3, 645–648.
Xiao, X. Wang, Y. Zhang, L. and Rong, T. (2006). SSR marker-based genetic diversity fingerprinting of hybrid rice in Sichuan china. China Journal of Rice, 20, 1–7.
Yu, J. Dale, T. Singh, S. Bensher, D. Gill, B. and Sorrel, M. (2004). Development and mapping of EST derived SSR markers for hexaploid wheat genome. American Journal of Botany, 47, 805–818.
Zachary, J. (2010). Weed-An environmental history of metropolitan America. American Historical Review, 1619.
Zane, L. Bergelloni, L. Patarnello, T. (2002). Strategies for microsatellite isolation . Molecular Ecology, 11, 1–16.
Zhoau, X. (1993). Phylogenetic distribution and genetic mapping of a (GGC)n microsatellite from rice (Oryza sativa) . Plant Molecular Biology, 4, 607–614. Zhu, H. Choi, H. Cook, D. and Shoemaker, R. (2005). Bridging models and crop