CAPITULO IV PROPUESTA INDICADORES

conserved. A s with the E12 conservation data the E42 conservation data supports its potential involvement in ion uptake by the FwTBP-DNA complex.

The FwTBP residues K54, K71, K64, K140, K145, K155 and K162 are conserved as basic residues with the exception o f the halobacterium TBP proteins where K54, K71 K145 and K162 are replaced by neutral residues in some forms o f the protein. The conservation pattern o f these residues in the halophiles suggests that some adaptation to extreme salinity (>2 M NaCl) is gained from these changes. This is possibly brought about by the more negative surface electrostatic potential, which would result from the removal o f basic residues, or by the removal o f anion binding sites in the free form of the protein (see Section 4.9). Such observations suggest that TBP proteins from extreme halophiles would exhibit an even more positive salt dependence o f binding than the FwTBP.

Figure 4.2.2 Repeat 1

HI S2 S3 S4 85 H2

1 0 2 0 3 0 4 0 5 0 6 0 7 0 8 0 9 0

PYRWO MVDMSKVKLRIENIVASVDLFAQLDLEKVLDL PN SK Y N PEEFPG II HLDDPKVALLlfSSGKLW TGAKSVQDIERAVAKLAQKLKSIG PYRFU 100% MVDMSKVKLRIENIVASVDLFAQLDLEKVLDL PN SK Y N PEEFPG II HLDDPKVAL|»lfSSGKLWT<aAKSVQDIERAVAKLAQKLKSIG PYRHO 9 2 . 7 % MVDTSNVKLRIENIVASVDLFAQLDLEKVLDI PN SK Y N PEEFPG II RFDDPKVALXiIFSSGKLW TGAKSIQDIERAVAKLIQKLKGIG PYRAR 9 0 . 7 % MVDTNNVKLRIENIVASVDLFAQLDLEKVLDI PN SK Y N PEEFPG II RFDDPKVALLIFSSGKLW TOAKSIH DIERAVAKLIEKLKGIG PYRKO 8 4 . 1 % MVDMSNVKLRIENIVASVDLFTDLNLEKVIEI P SSK Y N P E E F P G II RFDDPKVALLIFSSGKLW TGAKSVDDIKRAVYKLIEM LKKIG THECE 84.0% MSNVKLRIENIVASVDLFTQLNLERVIEM PH SK YNPEEFPG II RFDEPKVALLIFSSGKLW TGAKSVEDIERAVNKLIQM LKKIG METTL 4 8 . 9 %--- EIKIVNW V STQ IG TDIDLEYAADILD NAEYEPEQ FPG LV RLSDPKVltLLlFRSGKLNCTGAKSKEDAEIAIKKIIKELKDAG THEAC 4 9 . 2 %--- KITIENIVASTSLAEHLDLSRIALALDGSEYEPEQFPGLIYRLQEPKTAVLIFRSGKVNCTGAKNIEDVKRTIKIIIDKLKAAD THEVO 4 9 . 2 %--- K ITIENIVASTSLAEH LDLSRIALALDG SEYEPEQ FPG LIYRLQ DPK TAVtlFRSG K VNCTG AK NIEDVK RTIK IIIDK LK AAN METMA 47 . 0 % M SESSIKIENW ASTKLAEEFDLTVIESEFEGAEYNKQKFPGLVYRVSDPKAjkFliVFTSGKW CTGTiNVADVHTW GNLAKKLNSIG METJA 4 8 . 8 %--- EIKIVNVW STKIGDNIDLEEVAM ILENAEYEPEQFPGLV RLSVPKVALLIFRSGKVNCTGAKSKEEAEIAIKKIIKELKDAG

ARCFU 44 . 8 % MQDYKIKIENWASTQIGENIDLNKISREIKDSEYKPKQFPGLVLRTKEPKAjlALVFRSGKV^CTGSifeSVEDARRAVKQIVKMLKEIG

SULSH 4 8 . 1 %--- VNIENIVATVTLEQSLDLYAMERSIPNIEYDPDQFPGLIFRLEQPKVTALIFKSGKMW T?^AKSTEELIKAVKRIIKTLKKYG SULSO 4 8 . 1 %--- VNIENIVATVTLEQSLDLYAMERSIPNIEYDPDQFPGLIFRLEQPKVTALIFKSGKM W TOAKSTEELIKAVKRIIKTLKKYG E H A L N 145 . 9% M TD-PKETINIENW ASTGIGQELDLQSVM DL-EGADYDPEQFPGLVYRTQDPgSAALIFRSGKIVCTGAKSTDDVHESLRIVFDKLRELS METTH 4 8 . 3 % MTDVDIKIENIVASATLGKSIDLQTVAEALENVDFNREQFPGLVYKLKEPKTAALIFGSGKLVCTGAKSIEDSKRAIKLTVDMMRTMD PYROC 4 8 . 6 % KPTANIENIVATVSLDQTLDLNLIERSILTVEYNPEQFPGLVYRLDSPKVTAjEilFKSGKMiVTGAKSTRDLIEAVKKIVRNLKKHG SULAC 4 8 . 1 % KAW NIBNlVATVTLDQTLDLYAM ERSVPNVEYDPDQFPGLIFRLESPilTSLIFKSGKM iVTGAKSTDELIKAVKRIIKTLKKYG D H ALN145 . 2 %--- IQIENVVASTDLSQELALEQLATDLPGAEYNPGDFPGVIYRLDDPKS% TLIFE#GKAVCT#AgSVDDVHDAISIW EDLRDLG AERPE 44 .9% M EGLPKPEVKIENlVATVILENQLDLNLIETKIQDVDYNPDQFPGLVYRLESPRVTV^IFKSGKM f ITGpÜKSINQLIHWKKLLKAFADQG BH A LN 142 . 4 % --- IHIENW ASSDLGQELALDQLSTDLPGAEYNPEDFPGW YRLQEPKSAHüIpRÿGRVgCTGAKSVDDVHEALGIVFGDIRELG F H A L N 143 . 5 %--- IHIBNW ASSDLGQELALDQLATDLDGAEYNPEDFPGW YRLQEPKSATLIFRSGKW CTGAKSVDAVHDALEIVFDDLRELG

SULKO 4 9 . 0 %--- PNVEYDPDQFPGLIFRLEAPKVTSilFKRGKM W TGAKSTDELIKAVKRIIKTLKRYG

NOSIiO 39.8% MYRKSDILPALQEWATVNLNCKLDLKAIALRARNAEYNPKRFAAVIMSlRDPKTTALIFASGKMyVTGAKSEQTSKLAAQKFSRIIHKLG

DROME 4 0 . 1 %---QLQHlVSTVNLCCKLDLKKIALHARNAEYNPKRFAAVIMRlREPRTTALIFSSGiOyiVCTGAKSEDDSRLAARKYARIIQKLG GUITH 38.9% -VRANEITPNIQ H W STVSLG IQ LDLK RIA LK ARN AEYNPRRFAAV IM RlRDPK TÿA lilfSSG îa^fVTG AK SEDSA RVACK K YARIIQ RLG CANAL 4 2 . 4 %---LQMIVATVNLDCRLDLKTIALHARNAEYNPKRFAAVIMRlRDPKTTALliASGKMWTCUtKSEDDSKLASRKYARIIQKLG

Repeat 1 ACECL 37 ARTSF 41 YEAST 42 MAIZE238 SPOFR 41 BOMMO 41 ACACA 39 TETTH 41 CAEEL 41 SOYBN 40 PNECA 41 MAIZE138 TOBAC 39 PNECA 41 EMENI 42 ARATH 39 HUMAN 39 MESAU 39 MOUSE 39 CHICK 39 XENLA 39 MESCR 39 WHEAT 38 SI . HI S2 S3 S4 S5 H2 10 20 30 40 50 60 70 80 90 4 % ---L|^vySTVNLGCTLELKEIAMQARNAEYNPKE§|AAVIMHIRDP]RTTALIFGSGKMVCTaA$SEQDSRTAARKYAKIVQKLG

2 % ---L% I$tSTVNLGCRLDLKKIALQARNAEYNPH% AAVIM RIREP#T$A#I#S$G$M VC#0A0SEEDSRLAARKYARIVQKLG

9 TSA TSG IV PT L 0kiyA T V T L G C R L D L K T V A L H A R N A E Y N P^0A A V IM R IR E P#T ^A L lFA S(^[M V V #|A #SE D D SK L A SR K Y A R IIQ K IG

4%

6 % ---QL^iySTVNLNCKLDLKKIALHARNAEYNP»gÜ|AAVIM RIREPli'

6 % ---

2 % ---L#NlVSTVN LG CK LDLK NIALH ARNAEYNPR B#AAVIM RlREP#T$A#I#A$G #M #Cg^A#SEEASRLAARK YARIIQ K LG

3% 9%

8 % ---U

8% --- I^ iy A T W L D C R L D L K T IA L H A R N A E Y N P p |iA A V IM R lR E P |tT «A ;^ I*A S G fM »V |||A |{S E D D S K L A S R K Y A R I IQKLG

4% -V D L SkIV P T L ^ lV ST V N L D C K L D L K A IA L Q A R N A E Y N P ^ pA A V IM R lR E P |!T èA tliA $G pM |fC ?i^ ;M |SE Q Q SK L A A R K Y A R IIQ K L G

4% -V D L S k IV P T L ^ I^ S T V N L D C K L D L K A IA L Q A R N A E Y N P ^ p A A V IM R lR E P p T èA 4 ljA iG ^ M |c|j|A j|sE Q S S K L A A R K Y A R IIQ K L G

S % ---L ^ ltA T V N L D C R L D L K T IA L Q R R N A E Y N P |^ A A V IM Î|lR E p iT iA I,l|A |G |tM fv M A |S E D D S K L A S R K Y A R IIQ K L G

5 % ---L ^ iy A T V N L D C R L D L K T IA L H A R N A E Y N P ^ |A A V IM |^ IR E P iT ÏA ÎilèA |G |(M jîv i(;^ S E D D S K L A S R K Y A R IIQ K L G

3% PVDLTKIVPTL#I% tSTVN LDCK LDLK AIALQ ARN AEYNPl% 0AAVIM #IREP#T$A& lÈA#G #M 0C#A#SEH LSK LAARK YA RIVQ K LG

0% A S E S S G IV P Q lÆ liS T W L G C K L D L K T IA L R A R N A E Y N P iW A A V IM ilR E P iT |iA il|s|G iM ^ c fiA iS E E Q S R L A A R K Y A R V V Q K L G

0% - - E S S G I Y P Q u S l f STVNLGCKLDLKTIALRARNAEYNPÉWAAVIMf I R E p |T ^ A |l|s iG lM ic m ^ S E E Q S R L A A R K Y A R W Q K L G

0% --E SSG IV PQ L#I#STV N LG C K L D LK TIA LR A R N A EY N P^@ A A V I]v^IR EP^T& A 0I#S#G #M #C gm A ÊSEEQ SR LA A R K Y A R V V Q K LG

0% - -ESSG IVPQ Lm i^STVNLG CK LDLK TIALRARNAEYNP^gAAVIM ^IREP^T^Aÿim Sm G m M gCm ÊAgSEEQ SRLAARK YARVVQ K LG , 0% --ESSG IV PO LglësTV N LG C K LD LK T IA LR A R N A EY N P^gA A V IM ÈlR EP^T& A & im sgG ËM m cM A m SEEO SR LA A R K Y A R V V O K LG

8 % ---L S lfS T V N L D C K L D L K A IA L Q A R N A E Y N P fM A A V IM |lR E P |'I# A j|l|A ||G lM ic H A isE Q Q S K L A A R K Y A R IIQ K L G

1 % ---L 0B l#ST V N L D C K L D L K A IA L Q A R N A E Y N P^0A A V IM $IR E P0T #A #I#A 8G #M §C @ A #SE Q Q SK L A A R K Y A R IIQ K L G

s r ^ HI’ S2’ S3’ ^ S4’ ^ S5’ ^ H2’

gggg

000^

1 0 0 1 1 0 1 2 0 1 3 0 1 4 0 1 5 0 1 6 0 1 7 0 1 8 0 1 9 0 PYRWO VKFKRAPQIDVQNMVFSGDIGREFNLDWALTLPN EY EPE Q FPG V IY R V K E P»SV lIiL FSSG K IV SOAiSSEADAWEAVRKLLRELDKYGLLEEEEEEL PYRFU 100% VKFKRAPQIDVQNMVFSGDIGREFNLDWALTLPN EYEPEQFPGVIYRVKEP#S$I%,I4@SSGKIV SGAJÏSEADAWEAVRKLLRELDKYGLLEEEEEEL PYRHO 9 2 . 7 % VKFKRAPLIDVQNMVFSGDIGREFNLDNVALTLPN E Y E PE Q FPG V IY R V K E PR A #I^U !SSG K IV SOAKSEADAWEAVRKLLRELEKYGLIEEEEEEL PYRAR 9 0 . 7 % VKFKRAPLIDIQNM^FSGDIGREFNLDNVALTLPN EYEPEQFPGVIYRVKDPRAŸIfeljSSSGKIV SGAJÉSEADAWEAVRKLLRELEKYGLIEEEEEEL PYRKO 8 4 . 1 % AKFTREPQIDIQNMVFSGDIGMEFNLDAVALILPN E Y E PE Q FPG V IY R V K E PR A $I#L #SSG R lV SGAKSEQDAWEAVKKLLRELEKYGLIEEEEE-- THECE 8 4 . 0 % AKFSRAPQIDIQNMVFSGDIGMEFNLDAVALSLPN E Y E PE Q F P G V IY R V K E P R A V ltL fSSG K IV SSAKSEHDAWEAVRKLLRELEKYDLIGEGEEE- METTL 4 8 . 9 % MDIIDNPEVNVQNM |?ATADLGIEPNLDDIS-TLEGTEYEPEQFPGLVYRLSDP|tVfV|il|(GSGKVVIT!GLKKKDDAYLALDKILSTLKE LEEE--- THEAC 4 9 . 2 % IEV Y D D PQ IIV gJ|I|?A V Y D L E SE L N L T D IA M SL E N V E Y E PE Q PPG L V Y R V E E PirvfL il^G SG K W TQAKEESEIEQAVIKVKKELQKVGLI--- THEVO 4 9 . 2 % IEVY DDPDIIVQ NlÿAVYDLESELNLTDIAM SLENV EYEPEQ FPG LVYRVEEPRV|fLÉI^G SG EW TGA^EESEIEQAVIKVKKELQKVGLI--- METMA 4 7 . 0% IK TIED PQ ITV Q NIVASADLH TILNLNAIALG LENIEYEPEQ FPG LVYRIDEPRV$V$lFSSG K LW AG G gSPEDCERSVEW RQ Q LDSM G LL--- METJA 4 8 . 8 % IDVIENPEIKIQNM #ATADLGIEPNLDDIALM VEGTEYEPEQFPGLVYRLDDP#V#V$I#GSGKW I9#L˧SEEDAKRALKKIL--- ARCFU 4 4 . 8 %

SULSH 4 8 . 1 %

SULSO 4 8 . 1 % IK IV G K P K IQ l|^lfA SA N L H V N V N L D K A A FL L E N N M Y E PE Q PPG L IFlM D D PfV fL |lpSSG K M V ljrG A i<R E D E V SK A V K R IlA E L D C V K PIE E E E E -- E H A L N 145.9% IK V ED D PEIW Q i[l|?TSA D LG R Q LN LN A IA lG LEN IEY EPEQ FPG LV Y R LD D P]fV ||A |iI^G SG R LV lT0(^EPK D A EH A V D K ITSR LEELG LLD --- METTH 4 8 . 3 % PD IPEEFEIK I§#I#ASANLG K PLNLEA VALG LENTEYEPEQ FPG LVY#LDDP0VgL§L0G SG K VV TfiARSAEDAKLGVEKTLAELD--- PYROC 4 8 . 6 % IQ IY G R PK V Q lQ N IV A SA N LN V C V D LER A A LTLE N SM Y E PEQ FPG L IH rrviD EPirvfl4l||SSG p[V lTG A fR EEEV Y EA V N K IY EK LK K --- SULAC 4 8 . 1 % M QLTGKPKIQI0^I#ASANLHVIVNLDKAAFLLENNM YEPEQFPGLIYklM DEPRW L^I#SS(^M VI% pV$REDEVHKAVKKILVELDCVKPVEEEELE- D H A L N 145.2% IDIPPSPPVHVQNlVCSGSLDQDLNLNAIAlGLEDVEYEPEQFPGLVYT.LNDPDW VIiLFGSGèLVIÏ^GitNPDDAHHALEIIHERLTDLGLLE--- AERPE 4 4 . 9 % IP IS G K P Q IQ I^ I||A S A N L K V Y ID L E K A A L E F E N S L Y E P E (^ P G L IY MDEPRWM^l|SSG5KMVIîeiA|tMENEVYDAVKKVARKL--- BH A LN 142 . 4% ID V T S N P P IE V #I#S S A S L E Q S L N L N A IA lG L E Q IE Y E P E Q F P G L V Y LDDPDVVVgLgGSG^LVI^G^NPDEAEQALAHVQDRLTELGLLD--- FH A L N 143 .5% IDVDSTPPVKVQNiySSASLEQSLNLNAIAlGLEQIEYEPEQFPGLVY±:LDDPDVVV#LFGSG#LVlW A#ESADAQHALAHVNDRLTELGLLE--- SULKO 4 9 . 0 % MNLTGKPKIQIQNIVASANLHVIVNLDKAAFLLENNMYEPEQFPGLIY ayiEDPRVyi^liSSG^M VigSÛAKREEEVHKAVKKILVELDCVKPFEEEELE- NOSLO 3 9 . 8 % F N T K F A -D F K lïm i||S S C D T Q F S IR L E G L A F A H S N SYEPELFPGLIYi<MVKPX«IVLLl||VSG|?IVL®ftAKMRDEIYEAFDNIYPVLTQY---

DROME 4 0 . 1 % FPAKFL-DFKli^NMyGSCDVKFPIRLEGLVLTHCNSSYEPELFPGLIYiMVRPRiyLLlivSGKVVLT0A^VRQEIYDAFDKIFPILKKF---

GUITH 3 8 . 9 % YGHAKFIDFRIQNIVASCDVRFPIRLESLAHAHNQ SYEPELFPGLIY#MITPX'CVyL;g,I#VSGRLVL$GAmQRMDIFQAFSNIYSVLCLY--- CANAL 4 2 . 4 % F N A K F C -D F K I^ l||G S T D V K F A IR L E G L A F A H G T S S Y E P E L F P G L IY |tM V K P |lfI^ lf^ S G tlV L T O A E K R E D A F E S IY P V L N E F R K ---

s r ^ HI’ S2’ S3’ S4’ ^ S5’ H2’

1 0 0 1 1 0 1 2 0 1 3 0 1 4 0 1 5 0 1 6 0 1 7 0 1 8 0 1 9 0