1.3. OBJETIVOS
2.3.6 Concepto de placenta previa
2.3.6.8 Clasificación de la placenta previa
8.1 FUTURE RESEARCH
The results of this research demonstrate a need to expound on the use of fluorescence microscopy in fate and transport studies and the influence of individual SOM components within soil matrices. Below is a bulleted list of my suggested experiments.
• The contaminated synthetic and natural soils should be observed under confocal laser scanning microscopy (CLSM). This would potentially provide the researcher with a three-dimensional view of the soil particle surface coverage compared to what a simple fluorescence microscope could illustrate. CLSM may also allow examination of pores and intra-particle spaces, and the HS and PAH sorption phenomena therein.
• Another experiment would involve use of Q-dots, which are small, bright, fluorescent probes with specific excitation and emission wavelengths primarily used for
immunohistochemistry stains. Attaching Q-dots to various SOM components and PAHs would allow a greater number of constituents to be visualized concurrently and, thus, generate a more complete soil map. In addition, use of Q-dots in a static equilibration system would permit the researcher to view contaminant progression more clearly, as autofluorescence is only bright at medium to high concentrations.
• Glomalin, a ubiquitous glycoprotein coating hyphae of arbuscular mycorrhizal fungi, is rapidly becoming known for its profound impact on particle aggregation. Therefore, perhaps this biological constituent should be evaluated for its impact on contaminant sorption and compare the results to those found for PG.
8.2 IMPROVEMENTS
As with any project, reflection is a prime means of improvement. Therefore, the following is a list of improvements that may greatly assist those embarking on the heels of my research.
• The entire scale of the flow cell should be increased, as this would decrease the amount of clogging within the system.
• The flow cell should be made using a square-welled microscope slide. This would yield a greater surface area to observe flow, make sample preparation easier, and eliminate fluorescence spreading, caused by the curvature of the capillary tube. The accompanying coverslip could either be super-glued or wax-sealed to the slide well; therefore, it can be removed, and the slide re-used.
• To prevent clogging of the flow cell exit tubing and formation of buffer eddies within the entry tubing, TEM screens could be inserted. This would also keep the fluorescence detector from becoming clogged by particles dislodging from the flow cell.
• Remove as many junctions in the flow cell system as possible as these are a common source of leaks.
• Install a factory-built camera shutter or amend the current version to operate more accurately with the Microfire fluorescence microscope software.
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