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4. Resultados

4.2. Concepciones de conflicto identificadas durante la práctica educativa

Armstrong, D.S., Grimwood, K., Carlin, J.B., Rosemary, C., Gutièrrez, J.P., Hull, J., Olinsky, A., Phelan, E.M., Robertson, C.F., Phelan, P.D. Lower airway inflammation in infants and young children with cystic fibrosis. Am.J.Respir.Crit.Care Med. (1997) 156:1197- 1204.

Ballard, P.L., Merrill, J.D., Godinez, R.I., Godinez, M.H., Truog, W.E., Ballard, R.A. Surfactant protein profile of pulmonary surfactant in premature infants. Am.J.Resp.Crit.Care Med. (2003) 168:1123-1128.

Bligh, E.C., Dyer, W.J. A rapid method of total lipid extraction and purification. Can.J.Biochem.Physiol. (1959) 37:911-917.

Bradford, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal.Biochem. (1975) 72:248-254.

von Bredow, C., Birrer, P., Griese, M. Surfactant protein A and other bronchoalveolar lavage proteins are altered in cystic fibrosis. Eur.Respir.J. (2001) 17:716-722.

von Bredow, C., Wiesener, A., Griese, M. Proteolysis of surfactant protein D by cystic fibrosis relevant proteases. Lung (2003) 181:79-88.

Bufler, P., Schmidt, B., Schikor, D., Bauernfeind, A., Crouch, E.C., Griese, M... Surfactant protein A and D differently regulate the immune response to nonmucoid Pseudomonas aeruginosa and its lipopolysaccharide. Am.J.Respir.Cell Mol.Biol. (2003) 28:249-256. Cassidy, K.J., Halpern, D., Ressler, B.G., Grotberg, J.B. Surfactant effects in model airway

closure experiments. J.Appl.Physiol. (1999) 87(1):415-427.

Crouch, E.C. Collectins and pulmonary host defense. Am.J.Respir.Cell Mol.Biol. (1998) 19:177-201.

Donaldson, S.H., Boucher, R.C. Update on pathogenesis of cystic fibrosis lung disease. Curr Opin.Pulm.Med. (2003) 9:486-491.

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Enhorning, G. Pulsating bubble technique for evaluating pulmonary surfactant. J.Appl.Pysiol. (1977) 43(2):198-203.

Enhorning, G., Holm, B.A. Disruption of pulmonary surfactant’s ability to maintain openness of a narrow tube. J.Appl.Physiol. (1993) 74(6):2922-2927.

Enhorning, G. Pulmonary surfactant function in alveoli and conducting airways. Can.Respir.J. (1996) 3:21-27.

Literaturverzeichnis 190

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Folch, J., Lees, M., Stanley, G.H.S. A simple method for the isolation and purification of total lipids from animal tissues. J.Biol.Chem. (1957) 226:497-509.

Frerking, I., Günther, A., Seeger, W., Pison, U. Pulmonary surfactant: functions, abnormalities and therapeutic options. Intensive Care Med. (2001) 27:1699-1717. Gardai, S.J., Xiao, Y.Q., Dickinson, M., Nick, J.A., Voelker, D.R., Greene, K.E., Henson,

P.M. By binding SIRPalpha or calreticulin/CD91, lung collectins act as dual function surveillance molecules to suppress or enhance inflammation. Cell (2003) 115:13-23. Gibson, R., Burns, J., Ramsey B.W. Pathophysiology and management of pulmonary

infections in cystic fibrosis. N.Engl.J.Med. (2003) 168:918-951.

Griese, M., Birrer, P., Demirsoy, A. Pulmonary surfactant in cystic fibrosis. Eur.Respir.J. (1997) 10:1983-1988.

Griese, M. Pulmonary surfactant in health and lung diseases: state of the art. Eur.Respir.J. (1999) 13:1455-1476.

Griese, M., von Bredow, C., Birrer, P. Reduced proteolysis of surfactant protein A and changes of the bronchoalveolar lavage fluid proteome by inhaled a1-protease inhibitor in cystic fibrosis. Electrophoresis (2001) 22:165-171.

Griese, M., Neumann, M., von Bredow, C., Schmidt, R., Ratjen, F. Surfactant in healthy children and in children with malignancies, immunosuppression, fever and pulmonary infiltrates. Eur.Respir.J. (2002) 20:1284-1291.

Griese, M. Surfactant. Kapitel 1.4.1. in Pädiatrische Pneumologie, 2. Aufl. (2004) 63-72. Hrsg. Rieger, C., Von der Hardt, H., Sennhauser, F.H., Wahn, U., Zach. Springer, Berlin.

Hohlfeld, J., Fabel, H., Hamm, H. The role of pulmonary surfactant in obstructive airways disease. Eur.Respir.J. (1997) 10:482-491.

Hull, J., South, M., Phelan, P., Grimwood, K. Surfactant composition in infants and young children with cystic fibrosis. Am.J.Respir.Crit.Care Med. (1997) 156:161-165.

Jarjour, N.N., Enhorning, G. Antigen induced airway inflammation in atopic subjects generates dysfunction of pulmonary surfactant. Am.J.Resprir.Crit.Care Med. (1999)160:336-341.

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Kraemer, H.J., Schmidt, R., Günther, A., Becker, G., Suzuki, Y., Seeger, W. ELISA technique for quantification of surfactant protein B (SP-B) in bronchoalveolar lavage fluid. Am.J.Respir.Crit.Care Med. (1995) 152:1540-1544.

Kuroki, Y., Voelkers, D.R. Pulmonary Surfactant Proteins. J.Bio.Chem. (1994) 269:25943- 25946.

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Mander, A., Langton-Hewer, S., Bernhard, W., Warner, J.O., Postle, A.D. Altered phospholipid composition and aggregate structure of lung surfactant is associated with impaired lung function in young children with respiratory infections. Am.J.Respir.Cell Mol.Biol. (2002) 27:714-721.

Meyer, K.C., Sharma, A., Brown, R., Weatherly, M., Moya, F.R., Lewandoski, J.R., Zimmerman, J. Function and composition of pulmonary surfactant and surfactant- derived fatty acid profiles are altered in young adults with cystic fibrosis. Chest (2000) 118:164-174.

Noah, T., Murphy, P.C., Alink, J.J., Leigh, M.W., Hull, W.M., Stahlman, M.T., Whitsett, J.A. Bronchoalveolar lavage fluid SP-A and SP-D are inversely related to inflammation in early cystic fibrosis. Am.J.Respir.Crit.Care Med. (2003) 168:685-691.

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Postle, A.D., Mander, A., Reid, K.M., Wang, J.Y., Wright, S., Moustaki, M., Warner, J. Deficient hydrophilic lung surfactant proteins A und D with normal surfactant

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Robinson, T.E., Leung, A.N., Northway, W.H., Blankenberg, F.G., Chan, F.P., Bloch, D.A., Holmes, T.H., Moss, R.B.. Composite spirometric- computed tomography outcome measure in early cystic fibrosis lung disease. Am.J.Respir.Crit.Care Med. (2003) 168:588-593.

Van Rozendaal, B., van-de Lest, C., van Eijk, M., van Golde, L.M.G., Voorhout, W.F., Van Helden, H., Haagsman, H. P. Aerosolized endotoxin is immediately bound by pulmonary surfactant protein D in vivo. Biochim.Biophys.Acta (1999) 1454:261-269. Rubio, F., Cooley, J., Accurso, F.J., Remold- O’Donnel, E. Linkage of neutrophil serine

proteases and decreased surfactant protein-A (SP-A) levels in inflammatory lung disease. Thorax (2004) 59(4):318-321.

Schmidt, R., Steinhilber, W., Ruppert, C., Daum, C., Grimminger, F., Seeger, W., Gunther, A. An ELISA technique for quantification of surfactant apoprotein (SP)-C in bronchoalveolar lavage fluid. Am.J.Respir.Crit.Care Med. (2002) 165(4):470-474. Wright, J.R. Immunomodulatory functions of surfactant. Physiol.Reviews (1997) 77:931-961. Wu, H., Kuzmenko, A., Wan, S., Schaffer, L., Weiss, A., Fisher, J.H., Kim, K.S.,

McCormack, F.X. Surfactant proteins A and D inhibit the growth of Gram-negative bacteria by increasing membrane permeability. J.Clin.Invest. (2003) 111:1589-1602.

Danksagung 193

Danksagung

Ich möchte mich an dieser Stelle ganz herzlich bei meinem Betreuer Herrn Prof. Dr. med Matthias Griese bedanken, der mich die gesamte Zeit hervorragend beraten und unterstützt hat. Ohne ihn wäre diese Arbeit nicht möglich gewesen.

Ein herzlicher Dank geht auch an alle Mitarbeiter der Forschungsgruppe Lunge, München und im Besonderen an Frau Andrea Schams, die bei Problemen im Labor immer mit Rat und Tat zur Seite stand.

Ebenso möchte ich mich bei den Zentren der BEAT- Studie für ihre Kooperation bedanken: Prof. Dr. med. Karl Paul, Klinikum für Pädiatrie der Charité, Berlin

PD Dr. med. Felix Ratjen, Zentrum für Kinderheilkunde, Essen

Dr. med. M. Ballmann, Kinderklinik der medizinischen Hochschule Hannover Dr. med. Ernst Rietschel, Kinderklinik der Universität zu Köln

Besonders möchte ich mich der Arbeitsgruppe von Dr. Reinhold Schmidt, Zentrum für Innere Medizin, Justus-Liebig-Universität Giessen für die Messung der SP-B und -C Konzentrationen danken.

Lebenslauf 194

Lebenslauf

Robert Karl Essl

geboren am 17.05.1977 in München, römisch-katholisch, ledig. Schulbildung 1983 - 1987 Grundschule, Taufkirchen 1987 - 1996 Gymnasium, Unterhaching

Abschluss: Allgemeine Hochschulreife.

Zivildienst

1996 - 1997 Kinderpoliklinik, Ludwig-Maximilian-Universität München, Ambulanz für Pneumologie und Allergologie.

Studium

1997 - 2004 Studium der Humanmedizin an der Universität Leipzig: 30.08.1999 Ärztliche Vorprüfung,

29.08.2000 Erster Abschnitt der Ärztlichen Prüfung, 11.09.2002 Zweiter Abschnitt der Ärztlichen Prüfung, 07.05.2004 Dritter Abschnitt der Ärztlichen Prüfung.

Ärztliche Tätigkeit

Seit 01.08.2004 Asklepios Kreiskrankenhäuser Weißenfels-Hohenmölsen GmbH, Klinik für Innere Medizin.

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