RM 2-Way ANOVA - VEHICLE - Control vs CCA Stim Source of Variation
2 Days 7 Days 2 Days 7 Days
Interaction Days CCA Matching
Bonferroni Post Hoc Test - VEHICLE - Control vs CCA Stim
RM 2-Way ANOVA - TUDCA- Control vs CCA Stim Source of Variation
Interaction Days
n VEH TUD VEH TUD
1 1.648 1.593 1.502 1.165 2 1.163 1.135 2.087 1.681 3 1.052 1.429 1.562 1.351 4 0.917 1.178 1.313 1.878 5 1.04 0.841 1.343 1.854 6 0.813 0.912 1.309 1.973 7 1.252 0.934 1.59 1.73 8 2.278 1.324 1.496 1.901 X 1.27 1.16825 1.52525 1.69163 SEM 0.169 0.09463 0.0894 0.10169 2 Days 7 Days
CPN10 Protein - CCA Stim/Cotnrol
P value Summary Significant?
0.2659 ns No 0.0027 ** Yes 0.7879 ns No Difference t Summary 0.1021 0.6106 ns -0.2549 1.524 ns -0.4213 2.519 ns -0.357 2.135 ns -0.5234 3.129 * -0.1664 0.9948 ns 2 Days :TUD vs. 7 Days :TUD P < 0.05 7 Days :VEH vs. 7 Days :TUD P > 0.05 2 Days :VEH vs. 7 Days :VEH P > 0.05 2 Days :VEH vs. 7 Days :TUD P > 0.05 2 Days :TUD vs. 7 Days :VEH P > 0.05
Bonferroni Post Hoc Test - MULTIPLE COMPARISONS - Control vs CCA Stim Comparison P value
2 Days :VEH vs. 2 Days :TUD P > 0.05
Interaction Days TUDCA
2-Way ANOVA - RELATIVE CHANGE - CCA Stim/Control Source of Variation
Table 6C: Protein expression of Uqcrc2 following TUDCA/vehicle treatment with 2 or 7 days of CCA.
P value Summary Significant?
n CTR STM CTR STM CTR STM CTR STM 0.888 ns No 1 0.9757 1.0663 0.775 1.491 0.2873 0.7757 0.6121 1.2933 0.2868 ns No 2 0.2208 0.4578 0.4772 0.9282 0.1247 0.1744 0.9401 1.1358 < 0.0001 **** Yes 3 0.6119 1.0395 0.8522 1.1477 0.5444 0.613 0.8225 0.85 0.0332 * Yes 4 0.3453 0.6345 0.4189 0.9534 0.6581 0.7583 0.8349 0.934 5 0.5319 1.2056 0.5053 1.0403 0.6159 1.6533 0.2752 0.8361
6 0.4219 0.6741 0.4532 0.5548 0.6269 0.7067 0.4873 0.5208 Days Difference t P value Summary
7 0.2599 1.2367 0.9255 1.0955 0.751 1.038 0.5942 1.3723 2 -0.421 4.177 P < 0.05 **
X 0.4811 0.9021 0.6296 1.0301 0.5155 0.8171 0.6523 0.9918 7 -0.4005 3.974 P < 0.05 **
SEM 0.098 0.1166 0.0805 0.1061 0.0851 0.1704 0.0872 0.1119
P value Summary Significant?
Difference t P value Summary 0.84 ns No -0.0344 0.2205 P > 0.05 ns 0.29 ns No -0.1485 0.9521 P > 0.05 ns 0.0044 ** Yes 0.085 0.545 P > 0.05 ns 0.0758 ns No -0.128 0.8207 P > 0.05 ns
-0.1369 0.8773 P > 0.05 ns
-0.1747 1.12 P > 0.05 ns Days Difference t P value Summary -0.0227 0.1456 P > 0.05 ns 2 -0.3016 2.327 P > 0.05 ns 0.0384 0.2459 P > 0.05 ns 7 -0.3394 2.618 P < 0.05 * 2:TUD STM vs. 7:TUD STM
7:VEH CON vs. 7:TUD CON 7:VEH STM vs. 7:TUD STM
2:VEH STM vs. 2:TUD STM Matching
2:VEH STM vs. 7:VEH STM
2:TUD CON vs. 7:TUD CON Bonferroni Post Hoc Test - TUDCA - Control vs CCA Stim
Bonferroni Post Hoc Test - MULTIPLE COMPARISONS - Control vs CCA Stim Comparison
2:VEH CON vs. 2:TUD CON
2:VEH CON vs. 7:VEH CON CCA
Uqcrc2 Protein - Control vs. CCA Stim
VEHICLE TUDCA RM 2-Way ANOVA - VEHICLE - Control vs CCA Stim Source of Variation
2 Days 7 Days 2 Days 7 Days
Interaction Days CCA Matching
Bonferroni Post Hoc Test - VEHICLE - Control vs CCA Stim
RM 2-Way ANOVA - TUDCA- Control vs CCA Stim Source of Variation
Interaction Days
n VEH TUD VEH TUD
1 1.093 2.7 1.924 2.113 2 2.074 1.399 1.945 1.208 3 1.699 1.126 1.347 1.033 4 1.838 1.152 2.276 1.119 5 2.266 2.684 2.059 3.038 6 1.598 1.127 1.224 1.069 7 4.758 1.382 1.184 2.309 X 2.189 1.65286 1.70843 1.69843 SEM 0.451 0.27179 0.16817 0.29898 2 Days 7 Days
Uqcrc2 Protein - CCA Stim/Cotnrol
P value Summary Significant?
0.4102 ns No 0.4949 ns No 0.3929 ns No Difference t Summary 0.5366 1.208 ns 0.481 1.083 ns 0.491 1.105 ns -0.0556 0.1251 ns -0.0456 0.1026 ns 0.01 0.02251 ns 2 Days :TUD vs. 7 Days :TUD P > 0.05 7 Days :VEH vs. 7 Days :TUD P > 0.05 2 Days :VEH vs. 7 Days :VEH P > 0.05 2 Days :VEH vs. 7 Days :TUD P > 0.05 2 Days :TUD vs. 7 Days :VEH P > 0.05
Bonferroni Post Hoc Test - MULTIPLE COMPARISONS - Control vs CCA Stim Comparison P value
2 Days :VEH vs. 2 Days :TUD P > 0.05
Interaction Days TUDCA
2-Way ANOVA - RELATIVE CHANGE - CCA Stim/Control Source of Variation
Table 6D: Protein expression of autophagy markers following TUDCA/vehicle treatment with 2 or 7 days of CCA.
P value Summary Significant?
n CTR STM CTR STM CTR STM CTR STM 0.0929 ns No 1 0.6866 0.9562 1.2332 1.5689 0.8754 2.0209 1.373 1.1471 0.9307 ns No 2 1.1338 1.6761 0.8139 0.8418 0.9243 1.0935 1.0527 1.4119 0.0853 ns No 3 1.3134 1.6527 1.4971 1.1799 1.1734 1.8217 0.9 1.343 0.0002 *** Yes 4 0.4677 0.666 0.4613 0.6809 0.7539 1.168 0.6132 0.8277 5 0.5058 0.6971 0.4575 0.5821 0.599 0.865 0.4345 0.4852
6 0.8275 0.8513 0.8718 0.3284 0.498 0.9678 1.1535 1.5923 Days Difference t P value Summary
7 0.3664 0.5445 0.6975 0.8735 0.4189 0.6178 0.7062 0.6416 2 -0.249 2.617 P < 0.05 *
X 0.6626 1.0063 0.754 0.8651 0.6554 1.2221 0.7791 1.0641 7 -0.0033 0.03489 P > 0.05 ns
SEM 0.1502 0.1771 0.1661 0.1541 0.1276 0.1936 0.1547 0.1584
P value Summary Significant?
Difference t P value Summary 0.09 ns No 0.0083 0.0389 P > 0.05 ns 0.9666 ns No -0.1045 0.489 P > 0.05 ns 0.0018 ** Yes -0.2158 1.01 P > 0.05 ns 0.0026 ** Yes 0.1412 0.6608 P > 0.05 ns
-0.1415 0.6621 P > 0.05 ns
0.158 0.7396 P > 0.05 ns Days Difference t P value Summary
-0.0287 0.1342 P > 0.05 ns 2 -0.4731 4.121 P < 0.05 ** -0.199 0.9316 P > 0.05 ns 7 -0.1737 1.513 P > 0.05 ns 2:TUD STM vs. 7:TUD STM
7:VEH CON vs. 7:TUD CON 7:VEH STM vs. 7:TUD STM
2:VEH STM vs. 2:TUD STM Matching
2:VEH STM vs. 7:VEH STM
2:TUD CON vs. 7:TUD CON Bonferroni Post Hoc Test - TUDCA - Control vs CCA Stim
Bonferroni Post Hoc Test - MULTIPLE COMPARISONS - Control vs CCA Stim Comparison
2:VEH CON vs. 2:TUD CON
2:VEH CON vs. 7:VEH CON CCA p62 Protein - Control vs. CCA Stim
VEHICLE TUDCA RM 2-Way ANOVA - VEHICLE - Control vs CCA Stim Source of Variation
2 Days 7 Days 2 Days 7 Days
Interaction Days CCA Matching
Bonferroni Post Hoc Test - VEHICLE - Control vs CCA Stim
RM 2-Way ANOVA - TUDCA- Control vs CCA Stim Source of Variation
Interaction Days
n VEH TUD VEH TUD
1 1.393 2.309 1.272 0.835 2 1.478 1.183 1.034 1.341 3 1.258 1.552 0.788 1.492 4 1.424 1.549 1.476 1.35 5 1.378 1.444 1.272 1.117 6 1.029 1.943 0.377 1.38 7 1.486 1.475 1.252 0.909 X 1.349 1.63643 1.06729 1.20343 SEM 0.061 0.14056 0.14147 0.09571 2 Days 7 Days
p62 Protein - CCA Stim/Cotnrol
P value Summary Significant?
0.517 ns No 0.0047 ** Yes 0.0774 ns No Difference t Summary -0.287 1.77 ns 0.2821 1.74 ns 0.146 0.9002 ns 0.5691 3.509 * 0.433 2.67 ns -0.1361 0.8394 ns 2 Days :TUD vs. 7 Days :TUD P > 0.05 7 Days :VEH vs. 7 Days :TUD P > 0.05 2 Days :VEH vs. 7 Days :VEH P > 0.05 2 Days :VEH vs. 7 Days :TUD P > 0.05 2 Days :TUD vs. 7 Days :VEH P < 0.05
Bonferroni Post Hoc Test - MULTIPLE COMPARISONS - Control vs CCA Stim Comparison P value
2 Days :VEH vs. 2 Days :TUD P > 0.05
Interaction Days TUDCA
2-Way ANOVA - RELATIVE CHANGE - CCA Stim/Control Source of Variation
n CTR STM CTR STM CTR STM CTR STM P value Summary Significant? 1 0.4702 0.9213 0.5324 0.6221 0.8336 0.9733 0.6116 0.6239 0.7914 ns No 2 0.2321 0.3757 0.3971 0.8829 0.4576 0.4232 1.2508 0.9229 0.2992 ns No 3 0.3204 0.3433 0.4988 0.4437 1.1166 0.6296 0.3589 0.4028 0.2891 ns No 4 0.6297 0.4163 0.8418 0.4583 0.782 0.7959 0.2296 0.4209 0.0336 * Yes 5 0.9813 0.565 1.2649 0.9449 0.4025 0.2953 0.5844 0.3164 6 0.5357 0.4289 0.7453 0.5335 0.1739 0.2531 1.0657 0.8049
7 0.3309 0.5239 1.6659 1.0351 0.7824 0.6055 1.8978 0.8523 Days Difference t P value Summary
8 1.2385 0.5829 0.233 0.3004 0.1839 0.6949 0.8339 0.9762 2 0.07269 0.5885 P > 0.05 ns
X 0.5923 0.5197 0.7724 0.6526 0.5916 0.5839 0.8541 0.665 7 0.1198 0.9698 P > 0.05 ns
SEM 0.124 0.0652 0.1696 0.095 0.1196 0.0876 0.1917 0.0916
P value Summary Significant? Difference t P value Summary 0.3129 ns No
-0.18 0.9612 P > 0.05 ns 0.3051 ns No -0.18 0.9612 P > 0.05 ns 0.2752 ns No -0.1329 0.7098 P > 0.05 ns 0.0132 * Yes -0.1329 0.7098 P > 0.05 ns
-0.0817 0.4362 P > 0.05 ns
-0.0124 0.0664 P > 0.05 ns Days Difference t P value Summary
-0.0817 0.4362 P > 0.05 ns 2 0.00769 0.06277 P > 0.05 ns -0.0124 0.0664 P > 0.05 ns 7 0.1891 1.543 P > 0.05 ns
RM 2-Way ANOVA - TUDCA- Control vs CCA Stim Source of Variation Interaction Days Interaction Days CCA Matching
Bonferroni Post Hoc Test - VEHICLE - Control vs CCA Stim
LC3-II: LC3-I Protein - Control vs. CCA Stim VEHICLE TUDCA
RM 2-Way ANOVA - VEHICLE - Control vs CCA Stim Source of Variation
2 Days 7 Days 2 Days 7 Days
Bonferroni Post Hoc Test - MULTIPLE COMPARISONS - Control vs CCA Stim Comparison
2:VEH CON vs. 2:TUD CON
2:VEH CON vs. 7:VEH CON CCA
2:VEH STM vs. 2:TUD STM Matching
2:VEH STM vs. 7:VEH STM
2:TUD CON vs. 7:TUD CON Bonferroni Post Hoc Test - TUDCA - Control vs CCA Stim
2:TUD STM vs. 7:TUD STM 7:VEH CON vs. 7:TUD CON 7:VEH STM vs. 7:TUD STM
n VEH TUD VEH TUD
1 1.959 1.168 1.168 1.02 2 1.619 0.925 2.224 0.738 3 1.071 0.564 0.889 1.122 4 0.661 1.018 0.544 1.833 5 0.576 0.734 0.747 0.541 6 0.801 1.456 0.716 0.755 7 1.583 0.774 0.621 0.449 8 0.471 3.778 1.29 1.171 X 1.093 1.30213 1.02488 0.95363 SEM 0.198 0.36696 0.1943 0.15608
LC3-II: LC3-I Protein - CCA Stim/Cotnrol
2 Days 7 Days
P value Summary Significant?
0.5679 ns No 0.3987 ns No 0.778 ns No Difference t Summary -0.2095 0.61 ns 0.06775 0.1973 ns 0.139 0.4047 ns 0.2773 0.8072 ns 0.3485 1.015 ns 0.07125 0.2074 ns
2-Way ANOVA - RELATIVE CHANGE - CCA Stim/Control Source of Variation
Interaction Days TUDCA
Bonferroni Post Hoc Test - MULTIPLE COMPARISONS - Control vs CCA Stim Comparison P value
2 Days :VEH vs. 2 Days :TUD P > 0.05
2 Days :TUD vs. 7 Days :TUD P > 0.05 7 Days :VEH vs. 7 Days :TUD P > 0.05 2 Days :VEH vs. 7 Days :VEH P > 0.05 2 Days :VEH vs. 7 Days :TUD P > 0.05 2 Days :TUD vs. 7 Days :VEH P > 0.05