Conocimientos sobre actividad física y la salud de la espalda

a) b) c) d)

Figure 25 Standard method: Oils with one main zone (1)

Standards in order of increasing RF-value: Menthol R2000-03, Caryophyllene oxide R2224-02, Menthyl

acetate R1998-04, β-Caryophyllene R1996-06

a) Track 1: Standards; Track 2: Tea tree oil S2268-01; b) Track 1: Standards; Track 2: Thyme oil S2269- 01; c) Track 1: Standards; Track 2: Clove oil S2270-01; d) Track 1: Standards; Track 2: Coriander oil S2271-01

Table 15 Description of the chromatograms with one main zone (1)

Standards Tea tree oil Thyme oil Clove oil Coriander oil

pink zone (β- caryophyllene) blue zone (menthyl acetate) pink zone (caryophyllene oxide) blue zone (menthol) Start position

weak pink zone (β- caryophyllene)

Brownish-grey zone (terpinen-4- ol)

weak brown zone (terpineole) Start position

weak pink zone (β- caryophyllene)

orange zone (thymol) weak pink zone weak blue zone weak brown zone Start position

weak pink zone (β- caryophyllene)

dark grey zone (eugenol)

Start position

weak grey zone (geranyl acetate)

grey zone (linalol) two weak grey zones Start position

Tea tree oil has beside the weak β-caryophyllene zone on the top and the greyish zone on the start position the brownish-grey main zone of terpinen-4-ol above the menthol zone on the track with the corresponding standards. As shown in figure 25 a) and schematically in table 15 under the main zone also a weak brown zone may be visible.

The thymol zone above the caryophyllene oxide zone is the main zone of thyme oil, exposed in figure 25 b) and schematically in table 15. At the position of β-caryophyllene and caryophyllene oxide are also two pink zones visible, as well as a weak blue and a weak brown zone over and under the menthol zone.

Clove oil has a dark grey zone on beside the caryophyllene oxide zone on the first track with the standards. As displayed in figure 25 c) and schematically in table 15 also a weak β- caryophyllene zone is visible on the solvent front.

Coriander oil presents beside the linalol zone above the menthol zone three weak grey zones, one over the linalol and two under the linalol zone. Those results are shown in figure 25 d) and in table 15 as schematic description.

a) b) c) d) Figure 26 Standard method: Oils with one main zone (2)

Standards: Menthol R2000-03, Caryophyllene oxide R2224-02, Menthyl acetate R1998-04, β- Caryophyllene R1996-06; in order of increasing RF-value

a) Track 1: Standards; Track 2: Eucalyptus oil S2266-02; b) Track 1: Standards; Track 2: Caraway oil S2272-01; c) Track 1: Standards; Track 2: Nutmeg oil S2296-01; d) Track 1: Standards; Track 2: Cassia oil S2297-01

Table 16 Description of the chromatograms with one main zone (2)

Standards Eucalyptus oil Caraway oil Nutmeg oil Cassia oil

pink zone (β- caryophyllene) blue zone (menthyl acetate) pink zone (caryophyllene oxide) blue zone (menthol) Start position

grey zone (cineole)

weak grey zone

Start position

brown zone (carvone)

Start position

weak pink zone brown zone (myristicine) weak zone weak zone brown zone (terpinen-4-ol) weak zone Start position

weak pink zone

violet zone grey zone green zone some weak zones

Start position

Figure 26 a) shows the chromatogram of eucalyptus oil; well visibly are the cineole zone direct under the pink caryophyllene oxide zone and the weak grey zone under the menthol zone.

As displayed in figure 26 b) and table 16 caraway oil presents only one zone, the carvone zone beside the pink caryophyllene oxide zone.

Main zone of nutmeg oil is the myristicine zone above the menthyl acetate zone. Some other weak zone may be visible as exposed in figure 26 c) and table 16.

Cassia oil has a typical chromatogram with three nested zones beside the pink caryophyllene oxide zone as shown in figure 26 d) and table 16. Other weak zones may be visible under the nested zone.

a) b) c) d) Figure 27 Standard method: Oils with one main zone (3)

Standards: Menthol R2000-03, Caryophyllene oxide R2224-02, Menthyl acetate R1998-04, β- Caryophyllene R1996-06; in order of increasing RF-value

a) Track 1: Standards; Track 2; Anise oil S2292-01; b) Track 1: Standards; Track 2: Star anise oil S2293- 01; c) Track 1: Standards; Track 2: Fennel oil bitter S2294-01; d) Track 1: Standards; Track 2: Fennel oil sweet S2295-01

Table 17 Description of the chromatograms with one main zone (2)

Standards Anise oil Star anise oil Fennel oil bitter Fennel oil sweet

pink zone (β- caryophyllene) blue zone (menthyl acetate) pink zone (caryophyllene oxide) blue zone (menthol) Start position violet zone red zone (anethole)

weak zone weak zone weak zone

Start position

reddish zone weak violet zone red zone (anethole)

weak zone weak zone

Start position

red zone (fenchone)

Start position

red zone (fenchone)

weak zone

Start position

In figure 27 a) and b) as well as in table 17 were shown the chromatograms of anise oil respectively star anise oil. The differences were only weak; the chromatogram of star anise oil displayed additionally a weak violet zone above the red anethole zone, while the chromatogram of anise oil showed a weak brown zone beside the caryophyllene oxide zone. Likewise the chromatograms of bitter and sweet fennel, displayed in figure 27 c) and d) and schematically in table 17, oil exposed narrowly no difference; after derivatization at 366 nm was a faint zone visible under the caryophyllene oxide zone which was at 366 nm orange.