LA NATURALEZA EXPERIENCIAS
4.2. E LEMENTOS PARA UNA TEORÍA DE LA ÓPERA EN A RTEAGA
4.2.2. La cuestión del arte total
Supplemental Table 1
Primer sequences used for real-time PCR analysis.Gene product Forward (5’-3’) Reverse (5’-3’)
Pcbd1 TGGACATGGCCGGCAAGGC CCCACAGCCCTCAGGTTTG
Hnf1b CAAGATGTCAGGAGTGCGCTAC CTGGTCACCATGGCACTGTTAC
Gapdh TAACATCAAATGGGGTGAGG GGTTCACACCCATCACAAAC
Pcbd1: pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha; Hnf1b: hepatocyte nuclear factor b; Gapdh: glyceraldehyde 3-phosphate dehydrogenase.
Supplemental Table 2
Primer sequences used for cloning or mutagenesis PCR.Gene product Primer sequence (5’-3’)
PCBD1
HA- wild-type F CGACCGGTGGCTGGCAAGCACACAGG
R CGCTCGAGCTATGTCATGGACACTGCTAC Flag-wild-type F GCGCTAGCGCCACCATGGACTACAAGGATGACAAGGCTGGCAAA GCACACAGGCTG R CGCTCGAGCTATGTCATGGACACTGCTAC HA-Glu26Ter F GCTGTGGGGTGGAATTAGCTGGAAGGCCGTGAT R ATCACGGCCTTCCAGCTAATTCCACCCCACAGC HA-Thr78Ile F CACATCACGCTGAGCATCCATGAGTGTGCCGGC R GCCGGCACACTCATGGATGCTCAGCGTGATGTG HA-Cys81Arg F AGCACCCATGAGCGTGCCGGCCTTTCA R TGAAAGGCCGGCACGCTCATGGGTGCT HA-Glu86Ter F TGTGCCGGCCTTTCATAACGGGACATAAACCTG R CAGGTTTATGTCCCGTTATGAAAGGCCGGCACA HA-Arg87Gln F GCCGGCCTTTCAGAACAGGACATAAACCTGGCC R GGCCAGGTTTATGTCCTGTTCTGAAAGGCCGGC HA-Glu96Lys F CTGGCCAGCTTCATCAAACAAGTAGCAGTGTCC R GGACACTGCTACTTGTTTGATGAAGCTGGCCAG HA-Gln97Ter F GCCAGCTTCATCGAATAAGTAGCAGTGTCCATG R CATGGACACTGCTACTTATTCGATGAAGCTGGC
Role of PCBD1 in the HNF1B disease | 93
4
Supplemental Table 2
Continued.Gene product Primer sequence (5’-3’)
HNF1B Flag-wild-type F CGGCTAGCCCACCATGGACTACAAGGATGACGATGACAAGTGGC GCGCCGTGTCCAAG R GGCCCCCATTTGAACCGGTCG HA-wild-type F GGCGCGCCATGGTGTCCAAGCTCACGTCGC R TCTAGATCACCAGGCTTGTAGAGGACAC HA-Δ2-30 F CGGGCGCGCCGAGGAGTTGCTGCCATC R GGCCCCCATTTGAACCGGTCG HA-Lys156Glu F CCCAGCATCTCAACGAGGGCACCCCTATG R CATAGGGGTGCCCTCGTTGAGATGCTGGG HA-Gln253Pro F CAGGCCTACGATCGGCCAAAGAACCCCAGCAAG R CTTGCTGGGGTTCTTTGGCCGATCGTAGGCCTG HA-Arg276Gly F GCAGAATGTTTGCAGGGAGGGGTGTCCCCCTC R GAGGGGGACACCCCTCcCTGCAAACATTCTGC HA-His324Ser325fsdelCA F CTCCAACCAGACTCAGCCTGAACCCTCTGC R GCAGAGGGTTCAGGCTGAGTCTGGTTGGAG HA-Tyr352fsinsA F GTCAGGAGTGCGCTAACAGCCAGCAGGGAAAC R GTTTCCCTGCTGGCTGTTAGCGCACTCCTGAC
PCBD1: pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha; HNF1B: hepatocyte nuclear factor B.
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