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Muscle

• Muscle—a Latin word for ―little mouse‖ • Muscle is the primary tissue in the:

• Heart (cardiac MT)

• Walls of hollow organs (smooth MT)

• Skeletal muscle

• Makes up nearly half the body’s mass Overview of Muscle Tissue

• Functions of muscle tissue

• Movement

• Skeletal muscle—attached to skeleton

• Moves body by moving the bones

• Smooth muscle—squeezes fluids and other substances through hollow organs

Overview of Muscle Tissue

• Functions of muscle tissue (continued)

• Maintenance of posture—enables the body to remain sitting or standing

• Joint stabilization • Heat generation

• Muscle contractions produce heat

• Helps maintain normal body temperature Functional Features of Muscles

• Functional features

• Contractility

• Long cells shorten and generate pulling force • Excitability

• Electrical nerve impulse stimulates the muscle cell to contract • Extensibility

an opposing muscle • Elasticity

• Can recoil after being stretched Types of Muscle Tissue

• Skeletal muscle tissue

• Packaged into skeletal muscles • Makes up 40% of body weight • Cells are striated

Types of Muscle Tissue

• Cardiac muscle tissue—occurs only in the walls of the heart • Smooth muscle tissue—occupies the walls of hollow organs

• Cells lack striations

Similarities of Muscle Tissue

• Cells of smooth and skeletal muscle

• Are known as fibers

• Muscle contraction

• Depends on two types of myofilaments (contractile proteins) • One type contains actin

• Another type contains myosin

• These two proteins generate contractile force

Similarities of Muscle Tissues

• Plasma membrane is called a

sarcolemma

• Cytoplasm is called sarcoplasm

Skeletal Muscle

• Each muscle is an organ

• Consists mostly of muscle tissue • Skeletal muscle also contains

• Connective tissue • Blood vessels • Nerves

Basic Features of a Skeletal Muscle

• Connective tissue and fascicles

• Sheaths of connective tissue bind a skeletal muscle and its fibers together

• Epimysium—dense regular connective tissue surrounding entire muscle

• Perimysium—surrounds each fascicle (group of muscle fibers)

• Endomysium—a fine sheath of connective tissue wrapping each muscle cell

Basic Features of a Skeletal Muscle

• Connective tissue sheaths are continuous with tendons

• When muscle fibers contract, pull is exerted on all layers of connective tissue are tendon

• Sheaths provide elasticity and carry blood vessels and nerves Connective Tissue Sheaths in Skeletal Muscle

Basic Features of a Skeletal Muscle

• Nerves and blood vessels

• Each skeletal muscle supplied by branches of • One nerve

• One artery

• One or more veins

Basic Features of a Skeletal Muscle

• Nerves and blood vessels (continued)

• Nerves and vessels branch repeatedly • Smallest nerve branches serve:

• Individual muscle fibers

• Neuromuscular junction—signals the muscle to contract Basic Features of a Skeletal Muscle

• Muscle attachments

• Most skeletal muscles run from one bone to another • One bone will move, other bone remains fixed

• Origin—less movable attachment • Insertion—more movable attachment Basic Features of a Skeletal Muscle

• Muscle attachments (continued)

• Muscles attach to origins and insertions by CT • Fleshy attachments—CT fibers are short

• Indirect attachments—CT forms a tendon or aponeurosis • Bone markings present where tendons meet bones

• Tubercles, trochanters, and crests Microscopic and Functional Anatomy of Skeletal Muscle Tissue

• The skeletal muscle fiber

• Fibers are long and cylindrical

• Are huge cells—diameter is 10–100µm

• Length—several centimeters to dozens of centimeters • Each cell formed by fusion of embryonic cells

• Cells are multinucleate

• Nuclei are peripherally located Diagram of Part of a Muscle Fiber Myofibrils and Sarcomeres

• Striations result from internal structure of myofibrils • Myofibrils

• Are long rods within cytoplasm • Make up 80% of the cytoplasm

• Are a specialized contractile organelle found in muscle tissue • Are a long row of repeating segments called sarcomeres

(functional unit of Skeletal MT) Sarcomere

• Basic unit of contraction of skeletal muscle

• Z disc (Z line)—boundaries of each sarcomere

• Thin (actin) filaments—extend from Z disc toward the center of the sarcomere

• Thick (myosin) filaments—located in the center of the sarcomere

• Overlap inner ends of the thin filaments • Contain ATPase enzymes

Sarcomere Structure

• A bands—full length of the thick filament

• Includes inner end of thin filaments

• H zone—center part of A band where no thin filaments occur • A bands and I bands refract polarized light differently

• A bands—anisotropic • I bands—isotropic

Sarcomere Structure (continued)

• M line—in center of H zone

• Contains tiny rods that hold thick filaments together

• I band—region with only thin filaments

• Lies within two adjacent sarcomeres Sarcomere Structure (continued)

Sarcoplasmic Reticulum and T Tubules

• Sarcoplasmic reticulum

• A specialized smooth ER

• Interconnecting tubules surround each myofibril

• Some tubules form cross-channels called terminal cisternae • Cisternae occur in pairs on either side of a

t tubule

Sarcoplasmic Reticulum and T Tubules

• Sarcoplasmic reticulum

• Contains calcium ions—released when muscle is stimulated to contract

• Calcium ions diffuse through cytoplasm • Trigger the sliding filament mechanism

• T tubules—deep invaginations of sarcolemma

• Triad—T tubule flanked by two terminal cisterns Mechanism of Contraction

• Two major types of contraction

• Concentric contraction—muscle shortens to do work

• Eccentric contraction—muscle generates force as it lengthens • Muscle acts as a ―brake‖ to resist gravity

• ―Down‖ portion of a pushup is an example Mechanism of Contraction

• Sliding filament mechanism

• Explains concentric contraction

• Myosin head attach to thin filaments at both ends of a sarcomere

• Then pull thin filaments toward the center of the sarcomere

• Thin and thick filaments do not shorten

• Initiated by release of calcium ions from the SR • Powered by ATP

Sliding Filament Mechanism

• Contraction changes the striation pattern

• Fully relaxed—thin filaments partially overlap thin filaments • Contraction—Z discs move closer together

• Sarcomere shortens

• I bands shorten, H zone disappears • A band remains the same length Microscopic and Functional Anatomy of Skeletal Muscle Tissue

• Muscle extension

• Muscle is stretched by a movement opposite that which contracts it

• Muscle fiber length and force of contraction

• Greatest force produced when a fiber starts out slightly stretched • Myosin heads can pull along the entire length of the thin

filaments The Role of Titin

• Titin—a spring-like molecule in sarcomeres

• Resists overstretching

• Holds thick filaments in place • Unfolds when muscle is stretched Innervation of Skeletal Muscle

• Motor neurons innervate skeletal muscle tissue

• Neuromuscular junction is the point where nerve ending and muscle fiber meet

• Axon terminals—at ends of axons • Store neurotransmitters

• Synaptic cleft—space between axon terminal and sarcolemma The Neuromuscular Junction

Motor Units

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Muscle Tissue

Types of Skeletal Muscle Fibers

• Skeletal muscle fibers are categorized according to two characteristics

• How they manufacture energy (ATP) • How quickly they contract

• Oxidative fibers—produce ATP aerobically

• Glycolytic fibers—produce ATP anaerobically by glycolysis

Types of Skeletal Muscle Fibers

• Skeletal muscle fibers

• Are divided into three classes • Slow oxidative fibers

• Red slow oxidative fibers

• Fast glycolytic fibers

• White fast glycolytic fibers

• Fast oxidative fibers

• Intermediate fibers

Types of Skeletal Muscle Fibers

• Slow oxidative fibers

• Red color due to abundant myoglobin

• Obtain energy from aerobic metabolic reactions • Contain a large number of mitochondria

• Richly supplied with capillaries

• Contract slowly and resistant to fatigue • Fibers are small in diameter

Types of Skeletal Muscle Fibers

• Fast glycolytic fibers

• Contain little myoglobin and few mitochondria • About twice the diameter of slow-oxidative fibers

• Contain more myofilaments and generate more power • Depend on anaerobic pathways

Types of Skeletal Muscle Fibers

• Fast oxidative fibers

• Have an intermediate diameter

• Contract quickly like fast glycolytic fibers • Are oxygen-dependent

• Have high myoglobin content and rich supply of capillaries • Somewhat fatigue-resistant

• More powerful than slow oxidative fibers Disorders of Muscle Tissue

• Muscle tissues experience few disorders

• Heart muscle is the exception • Skeletal muscle

• Remarkably resistant to infection • Smooth muscle

• Problems stem from external irritants Disorders of Muscle Tissue

• Muscular dystrophy

• A group of inherited muscle destroying disease

• Affected muscles enlarge with fat and connective tissue • Muscles degenerate

• Types of muscular dystrophy

• Duchenne muscular dystrophy • Myotonic dystrophy

Disorders of Muscle Tissue

• Myofascial pain syndrome

• Pain is caused by tightened bands of muscle fibers

• Fibromyalgia

• A mysterious chronic-pain syndrome • Affects mostly women

• Symptoms—fatigue, sleep abnormalities, severe musculoskeletal pain, and headache

Muscle Tissue Throughout Life

• Muscle tissue develops from myoblasts

• Myoblasts fuse to form skeletal muscle fibers

Muscle Tissue Throughout Life

• Cardiac muscle

• Pumps blood three weeks after fertilization

• Satellite cells

• Surround skeletal muscle fibers

• Resemble undifferentiated myoblasts

• Fuse into existing muscle fibers to help them grow Muscle Tissue Throughout Life

• With increased age

• Amount of connective tissue increases in muscles • Number of muscle fibers decreases

• Loss of muscle mass with aging

• Decrease in muscular strength is 50% by age 80 • Sarcopenia—muscle wasting

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