Disposición del Modelo de Centro de Datos

Lymphocyte Transformation Test.

The results of two way analysis of variance show differences in the responses given by the vaccine groups. The p values show the level of significance of the difference, and the type of difference (i.e. whether it is greater or smaller) is shown from plots of the data and Student's t tests. The responses to all the antigens, with the exception of those of the group vaccinated with killed M.vaccae at six weeks of age to M.avium GWT, differed significantly over time as can also be seen from figures 6.1 - 6.6. In some cases there was

interaction between the two independent variables i.e. time and vaccine group, which then prevented the analysis being carried out.

The following results showed significant differences between the vaccine group and the control group:

(i) There was a significant difference in the response of the group receiving the low dose of killed vaccine at one day old to the M.fortuitum antigen (p < 0.04). (There was interaction in the responses to M.vaccae and M.avium RBP).

(ii) The group receiving the high dose of killed vaccine at one day old responded significantly differently to the M.vaccae antigen (p < 0.0005), the M.avium RBP antigen (p < 0.001) and the M.fortuitum antigen (p < 0.0001). (There was interaction in the response to M .avium GWT).

(iii) The group receiving the high dose of killed vaccine at six weeks of age responded significantly differently to the M.vaccae R877R antigen (p < 0.02) and the M.fortuitum antigen (p < 0.02). (There was interaction in the response to M.avium RBP).

(iv) The group receiving the live vaccine at six weeks of age showed no differences in response from the controls to any of the antigens.

The results of Student's t tests of vaccine groups' responses compared to the controls' responses are shown in table 6.4 and are summarised as follows:-

(i) the group receiving the low dose of killed vaccine at one day old gave significantly greater responses to M.avium RBP 100 ug/ml and M.fortuitum 50 ug/ml at 29 months of age.

(ii) the group receiving the high dose of the killed vaccine at one day old responded consistently better than control birds at many time points and to many antigens. At 26 months of age responses were

greater to BCG 50 ug/ml and both concentrations of M.fortuitum: 100

ug/ml and 50 ug/ml. Responses were greater to M.avium GWT 100 ug/ml at 29 months of age and again at 35 months of age to both concentrations. Also at this time there were greater responses to both concentrations of M.fortuitum. At 38 months of age there were greater responses to M.vaccae R877R 50 ug/ml, both concentrations of M.fortuitum and M.avium GWT 50 ug/ml. At 43 months, the greater responses were to both concentrations of M.fortuitum. However at 17 months of age the controls responded significantly better than this group to BCG 100 ug/ml.

(iii) the group receiving the high dose of killed vaccine at six weeks of age gave greater responses to both concentrations of M.avium GWT at 29 months of age and to BCG 100 ug/ml at 38 months of age.

(iv) the group receiving the live vaccine at six weeks of age responded significantly less than the control group to BCG 100 ug/ml at 17 months, but significantly more at 23 months of age to M.avium RBP 100 ug/ml and M.avium GWT 100 ug/ml at 29 months of age.

Results of M.vaccae vaccinated Mallard LTT responses are summarised in appendix 6.4 and LTT results to M.vaccae, M.avium and M.fortuitum are shown in figures 6.1 - 6.6.

Table 6.4 : Student's t tests of M.vaccae vaccinated Mallard LTT responses over time when compared to control birds' responses.

Months of age 17 23 26 29 35 38 43 Vaccine group Day 1, killed, lov dose. Day l, killed, high dose. 6 veek, killed, high dose. BCG 100 p < 0.025 LESS BCG 50 p < 0.01 I. fort 100 p < 0.05

i.tort

K.fort

p < 0.05 p < 0.05 M.a v i m GIT 50 M.fort 50 p < 0.05 p < 0.025 M.a v i m GIT 50 p < 0.05 p < 0.025 fort 50 p < 0.025 M.a v i m GIT 100 BCG 100 p < 0.025 p < 0.025 M.a v i m GIT 50 p < 0.05

6 veek, BCG 100 M.a v i m RBP 100 M . a v i m GIT 100 live, p < 0.01 p < 0.05 p < 0.01 high dose. LESS M.a v i m GIT 50

p < 0.05

(M.fort — M.fortuitum)

Key for figures 6.1 - 6.14.

Legend Vaccine group

dayl/k/low - 1 day old, low dose of killed M.vaccae dayl/k/high - " high dose " H 6week/k/high - 6 weeks old, high dose " " 6week/l/high * " " live M.vaccae

Figure 6.1

_{M.vaccae vaccinated Mallard LTT resposes}