DISTRITO INSTITUCIONES INVOLUCRADAS ACCIONES REALIZADAS

#​a _{Gene Annotation Mutation Change​}b _Freqc_{​ P-value​}d _Residue​e _{Protein effect​}f 1 pflu0458 GGDEF/EAL domain _protein Insertion + GTCA _GGTTG 87.2 1.8×10_​-183 ₄₆₉ 9 bp duplication

(D → DNLT)

2 wspA methyl-accepting _{chemotaxis protein} SNP T → G 100.0 1.0×10_​-213 ₄₄₁ Substitution

(V _​→​ G)

3 pflur18 23S ribosomal RNA SNP T → C 97.9 6.7×10​-8 _{- -}

Table 1. Mutations identified in TSS-f6 from whole-genome sequencing. _​a​_{# refers to the number of the}

mutation._​b​_{Change refers to the nucleotide sequence change. ​}c​_{Freq refers to the variant frequency (%). ​}d​_P-value

refers to the variant P-value. _​e​_{Residue refers to the amino acid residue number of the mutation in the protein. ​}f Protein effect refers to the change to the amino acid code of the protein.

#​a _{Gene Annotation Mutation Change​}b _Freqc_{​ P-value​}d _Residue​e _{Protein effect​}f 1 wspA methyl-accepting chemotaxis _protein SNP C → A 98.1 5.8×10​-315 ₁₀₄ Substitution

(T → N)

2 wspE

chemotaxis-related two- component system, sensor kinase

SNP A → T 98.6 7.1×10​-202 ₇₀ Substitution

(H → L)

Table 2. Mutations identified in f2 ancestor from whole-genome sequencing. _​a​_{# refers to the number of the}

mutation._​b​_{Change refers to the nucleotide sequence change. ​}c​_{Freq refers to the variant frequency (%). ​}d​_P-value

refers to the variant P-value. _​e​_{Residue refers to the amino acid residue number of the mutation in the protein. ​}f Protein effect refers to the change to the amino acid code of the protein.

#_​a _{Gene Annotation Mutation Change​}b _Freqc_{​ P-value​}d _Residue​e _{Protein effect​}f 1 19 bp​↓

pflu0046 - Insertion G(4) + G 100.0 1.0×10​

-80 _{- -}

2 pflu0046 hydrolase SNP A → G 100.0 2.5x10​-22 _{142 None}

3 fic cell filamentation protein SNP C → T 97.7 2.7x10​-129 _{164 None}

4 gpmI phosphoglycero mutase SNP C → G 100.0 4.0×10_​-47 ₃₀₀ Substitution

(A → P)

5 glnA glutamine synthetase SNP C → T 98.2 5.5×10_​-161 ₃₁₀ Substitution

(G → S)

Appendices

(R → C) 7 pflu0541 hypothetical protein Insertion G(6) + G 97.8 1.1×10_​-126 _{331 Frame Shift}

8 ureA urease subunit gamma SNP C → T 97.8 4.5×10_​-131 ₈₉ Substitution

(G → S) 9 prmA ribosomal protein L11 _{methyltransferase} SNP G → A 100.0 7.9×10_​-97 _{259 None}

10 dppA1 dipeptide ABC transporter _{substrate-binding protein} SNP T → C 100.0 5.0×10​-166 ₄₉₄ Substitution

(D → G) 11 pflu0872 modulation of DNA _{gyrase-like protein} SNP A → G 97.7 6.8×10​-121 _{410 None}

12 17 bp​↓

pflu0872 - Insertion + C 94.1 1.7×10​

-39 _{- -}

13 pflu0917 exported peptidase Insertion G(5) + G 100.0 3.2×10_​-218 _{173 Frame Shift}

14 hprA glycerate dehydrogenase SNP A → G 100.0 3.2×10​-102 _{239 None}

15 56 bp​↓

lpxC - Deletion T(10) − T 97.8 2.7×10​

-56 _{- -}

16 pflu1093 fimbrial outer membrane _{usher protein} Insertion G(8) + G 83.3 1.1×10_​-50 _{660 Frame Shift}

17 12 bp​↓

pflu1191 - Deletion T(9) − T 100.0 1.3×10​

-17 _{- -}

18 wspA methyl-accepting _{chemotaxis protein} SNP G → T 100.0 3.2×10_​-116 ₂₅₃ Substitution

(D → Y) 19 wspB chemotaxis-like protein Deletion − GCCG _AAGTG 100.0 2.5×10_​-58 ₃₇ Deletion

(− AEV) 20 wspF chemotaxis-specific _{methylesterase} SNP T → G 96.0 2.5×10_​-71 ₂₉₅ Substitution

(I → S) 21 pflu1490 GntR family _{transcriptional regulator} SNP A → G 100.0 1.3×10​-61 _{81 None}

22 pflu1593 hypothetical protein Insertion G(7) + G 97.7 1.1×10​-116 _{33 Frame Shift}

23 pflu1668

polysaccharide biosynthesis-related membrane protein

Insertion G(6) + G 93.8 1.7×10​-131 _{502 Frame Shift}

24 kdpB potassium-transporting _{ATPase subunit B} SNP C → T 97.1 1.4×10_​-104 _{397 None}

25 pflu1849 two-component system _{sensor kinase} Insertion G(8) + G 95.2 2.1×10_​-49 _{39 Frame Shift}

26 3 bp​↑ pflut43 - SNP T → A 93.3 9.5×10​ -39 _{- -} 27 1 bp​↑ pflut43 - Insertion + A 100.0 1.6×10​ -45 _{- -}

Appendices protein (G → S) 30 5 bp​↑ pflu2000 - SNP A → G 98.0 3.1×10​ -162 _{- -} 31 38 bp​↓ pflu2019 - SNP G → A 98.5 2.7×10​ -213 _{- -}

32 pflu2041 ABC transporter _{substrate-binding protein} SNP A → G 100.0 1.6×10_​-119 ₄ Substitution

(V → A) 33 pflu2079 polysaccharide exported- _{related lipoprotein} SNP C → T 100.0 3.2×10_​-78 ₈₇ Substitution

(D → N) 34 pflu2156 hypothetical protein SNP A → G 95.1 6.5×10​-111 ₈₈ Substitution

(S → P) 35 9 bp​↑

pflu2167 - SNP A → G 100.0 1.3×10​

-119 _{- -}

36 pflu2267 two-component system _{fusion protein} SNP A → G 100.0 1.0×10_​-87 _{1,059 None}

37 pflu2295 methyl-accepting _{chemotaxis protein} SNP A → G 100.0 2.0×10_​-57 ₃₆₃ Substitution

(D → G) 38 52 bp​↓

pflu2296 - Insertion G(7) + G 97.7 6.8×10​

-121 _{- -}

39 pflu2381 hypothetical protein Insertion G(9) + G 85.7 2.1×10_​-53 _{364 Frame shift}

40 pflu2732 hypothetical protein SNP C → T 95.0 4.0×10_​-62 _{254 None}

41 pflu2743 ABC transporter permease SNP G → A 100.0 2.0×10_​-84 _{50 None}

42 pflu2807 nicotinamide mono- _{nucleotide transporter} SNP A → G 91.7 2.8×10​-89 ₁₆₇ Substitution

(L → P) 43 pflu2947 ABC transporter peri- _{plasmic binding protein} SNP C → T 100.0 4.0×10​-65 ₂₈₃ Substitution

(G → R) 44 45 bp​↓

pflu3031 - Insertion C(6) + C 100.0 5.0×10​

-108 _{- -}

45 pflu3049 oxidoreductase SNP C → T 100.0 5.0×10_​-136 _{120 Truncation}

46 pflu3130

sensory box GGDEF/ EAL domain-containing protein SNP T → C 100.0 1.0×10​-58 _{275 None} 47 22 bp​↓ pflu3155 - Deletion − CC 100.0 1.6×10​ -41 _{- -} 48 18 bp​↓ pflu3157 - Insertion C(8) + C 92.9 6.0×10​ -84 _{- -}

49 pflu3409 methyl-accepting _{chemotaxis protein} SNP T → C 96.4 1.1×10​-85 _{187 None}

50 pflu3426 bifunctional reductase SNP G → A 96.2 8.2×10_​-67 ₆₄₂ Substitution

(E → K) 51 pflu3488 hypothetical protein Insertion C(6) + _C(2) 100.0 6.3×10_​-82 _{102 Frame Shift}

Appendices

pflu3647

53 pflu3728 ribose ABC transporter _{ATP-binding protein} SNP C → T 97.5 4.0×10_​-116 ₃₅ Substitution

(S → L) 54 pflu3951 substrate-binding _{periplasmic protein} Insertion C(7) + C 85.7 9.0×10_​-29 _{210 Frame shift}

55 pflu4017 amino acid ABC _{transporter permease} Insertion C(7) + C 98.2 1.8×10_​-158 _{25 Frame Shift}

56 pflu4042 pyridine nucleotide- _{disulfide oxidoreductase} Insertion C(6) + C 97.5 2.0×10​-104 _{236 Frame Shift}

57 pflu4306 GGDEF/GAF domain _{sensory box protein} SNP G → C 100.0 1.0×10​-62 ₁₃₅ Substitution

(A → P) 58 pflu4466 patatin-like phospholipase Insertion C(5) + C 100.0 1.0×10​-108 _{331 Frame Shift}

59 pflu4600 regulatory protein SNP T → C 100.0 2.0×10_​-96 ₁₅₇ Substitution

(Y → C) 60 pflu4769 hypothetical protein SNP G → A 100.0 1.6×10_​-125 ₅₇₆ Substitution

(A → T) 61 30 bp​↑

pflu4801 - SNP T → C 96.8 1.9×10​

-189 _{- -}

62 pflu4976 HlyD family secretion _protein Deletion C(8) − C 100.0 4.0×10​-51 _{304 Frame Shift}

63 27 bp​↓

pflu5147 - Insertion C(7) + C 100.0 6.3×10​

-162 _{- -}

64 pflu5210 signaling-related _{membrane protein} SNP G → A 100.0 1.0×10​-99 ₂₀₇ Substitution

(P → L) 66 pflu5210 signaling-related _{membrane protein} SNP T → G 100.0 1.0×10​-140 ₂₇ Substitution

(T → P) 67 pflu5213 exodeoxyribonuclease V _{subunit beta} SNP G → A 96.0 6.3×10_​-69 _{627 None}

68 pflu5329

sensory box GGDEF/ EAL domain-containing protein

SNP T → C 97.4 3.0×10_​-121 ₉₇₀ Substitution

(F → L) 69 pflu5329

sensory box GGDEF/ EAL domain-containing protein

SNP C → T 100.0 1.3×10_​-214 _{1,194 Truncation}

70 rpe ribulose-phosphate _3-epimerase SNP G → A 100.0 2.5×10​-172 ₁₅₉ Substitution

(R → C) 71 pflu5629 TonB-dependent _{membrane protein} SNP G → A 100.0 7.9×10​-159 ₇₃₂ Substitution

(G → S) 72 pflu5883 hypothetical protein SNP G → A 100.0 1.6×10​-129 ₅₂ Substitution

(D → N) 73 pflu5883 hypothetical protein SNP G → A 97.1 1.4×10_​-208 ₁₀₅ Substitution

(G → S) 74 pflu5978 hypothetical protein SNP T → C 97.8 4.6×10_​-125 ₃₆ Substitution

Appendices

75 pflu6104 hypothetical protein SNP G → A 100.0 4.0×10​-135 _{632 None}

Table 2. Mutations identified in f2 ancestor from whole-genome sequencing. _​a​_{# refers to the number of the}

mutation._​b​_{Change refers to the nucleotide sequence change. ​}c​_{Freq refers to the variant frequency (%). ​}d​_P-value

refers to the variant P-value. _​e​_{Residue refers to the amino acid residue number of the mutation in the protein. ​}f Protein effect refers to the change to the amino acid code of the protein.

In document articulacion un desafio permanente e indispensable Parte II pdf (página 31-39)