71 .
neede d to corrob o rate the evidence that sp erm transp o rt i s enhanc e d
by reffioving the re tardation imp o s ed by the c e rvix . I n the se exp eriment s there was no difference betv1e en one ins er.c.ination bef o re
o r
aftero vulation, or betvre en a sinele o r d o uble dose o f sp er:r,ato z o a . 'I'hi s c ould have b e e n a re sult of the large numbers o f
sp erm
dep o s ited o r that dep os ition a t the uterus re.r.':oved the eff e c t o f the c ervix where e ndo crinological effe c t s have their r:1a j o r iT'.:flue!'lc e . Further 7ro rk using variavle nw::tbers of sp erm p e r ins er::ination r�ight indi catethe limits to w: i c h fre shly ex t er.de d ram semen c ould b e used. I t is de s i rabl e tha-t future s tu ii e s o f spere transp o rt be s uppl emente:l 71i th lamb ing resul ": s a s the in utero viab il i ty of ova fertil i z ed by fro z e n sp ermatozoa ha s no t b een investigated.
The development of a suc c e s :.ful r::e thod of synchroniz ing
ovulation and ins e::cinatinc vri thout ob servation for o e s trus , ;!:icht 77i·:':.en the s c op e of artific ial b re eding of she ep in :�e-,7 Zealand , even.
-.i i
thout the use of frozer. semen. J iluted s emen uso:l on the lay of , or dayafter collection
ha s
b ee n very suc c e s sfully achieved in the dairy cattle indus try and such a tecl".nological devel opment '.'ii th ram s eJr;en, rr,ayhave application in F ew Zec.land .
Although the inj e ction of ECG rr�y not b e entirely s ati sfactory for the induction o f ovulations, the use of S)�thetic gonadotrophin rele as ing fac tors night be more promi
s
ing. P.ovrever, as the cervix o fthe ewe app ears t o b e the· s ite o f sper� transp o rt failure afte r o e s trus synchronization, the inseminating ins trument used in thi s s tudy might allow successful s et-time inseminations after p roge s tagen treatment , without the nee d for exp ensive ovulating ho�one s .
REF.E'lli!TCES
Adams , c .s .
( 1 969)
I ntrap eritoneal insemination in the rabbit .J . ?ert . ,
�: 333 .
Akester, A .�. ; I nk s ter, I . J .
( 1 961 )
C ineradiographic studie so� the genital tract o� the rabbit. J . �eprod . � :
507
72
Alli son, A . J .
( 1 972
a)
�he transp ort of spermatozoa in the spayedewe : a comp arison of two me tho ds o� steroid adwinistration.
J.
21 : 309
All i s on, A . J .
( 1 972
b)
C o mp arison o� spe rmato z oa transp ort in the spayed and ent ire e>ie . J . "..enrod.21 : 41 5
Allis on, A . J . ; �ob inson, T . J .
( 1 97C)
The effect o f dose levelo� intra-vaginal p rogestagen on sperm t ranspo rt , fertilization and lambing in the cyclic J.:erino eTie . J . C:.en rod . ?ert . ,
22 :
51 5
Allison, A . J . ; �ob ins on, T . J .
( 1 972 )
'::'he recover�.r of spe rmato z o afrom t.l-J.e rep roductive tract o f the spayed e;1e treated with progesterone ani o e s trogen. J •
l!_: 21 5
Andersen, :r: . ; .Aamdal , J . ; :::<'ougner, J . A .( 1 973 )
I ntra-uterineand deep cervical ins ecination Tiith fro z en secen in she ep. Zuch ,
�: 11 3
Anderson, D . H .
(1 928)
C omarative anator;:y of the tube-uterine j unction. �is tolo gy and physiology in the sow .Am. J .
2�2 : 255
Aus tin, C .R.
( 1 964)
B ehaviour of sp ermato z o a in the femaleg enital tract and in fertilization . ?ro e . V. C ongr . Anim.
l: 7
Austin, C .R.
( 1 975)
Sp erm fertility , viability and p ersistencein the feoale tract . "Fert . �urml .
22 ,
p .75 .
Bane , A.
( 1 952)
A study on the tedmiques o� haemocytometric determination of spe rm notility and sp erm c oncentration in bull s emen. C o rnell4 2 : 51 8
Bedford , J .t:.
( 1 971 )
�.ate of sperm pas sage into the c ervix73 .
Bellve , A . ll . ; !.:cDonald, ;.: .F .
( 1 968)
D irectional flow of Fall op iantub e
s e
cre
ti
onin
the
::l.onmey ewe . J ..12 = 357
Bickers , ',7 .
( 1 960)
Spe
rm �igr
ation a
ndu
teri
ne contr
ac tio
ns
. F ert . Steril.,1 1 :
286
B
is
hop,D .":: .
( 1 961 )
3io logy of sp ermato z oa .In
" <::ex
I nternal
t · lt 'T 1
2
707
(11'd '" '"' Y ' ·•· . 1, �
d
J.
o
ns . , . o . , p . • D . . . . v._ou
ng; • .. J.J.. ::i.2.r:-.s
an �illiams , Baltimo re .Boland, H . P . ; Go
r
don,I . ( 1 972)
Studie s in se t-time artific ialins eoination in sheep .
Fac .G- en .
Dub l i n ,1 972 ,
p .59 .
B
r
ad en,
A .� . H . ,( 1 953)
D i s tr ibution of s p e � s :n thec eni�al
tract of
the f enal e
rabb it afte
r coitus . �us t . J . ?: c i . ,6
�6S3
Braden, A .'·' . H . ; Aus tin, C . l..
( 1 9 51+-)
':"he number of s?e rr.:s
aboutthe e g G s
of marJ�al s andits
s ignifi�anc e for no�al:fertilizatio n .
Aus t . J . biol .Z:
5Lf-3B
r
aden, A ;-: . E . ; !.ame nd , � .� . • ; '"'.e.cl£'o r:: , �! .� : .( 1 960)
?he c entralo:f
the time ofovula tion
in sheep. Aust.
J . '"l_es . ,.!1. :
3 :,93
r
aden, A;.','.E.;:.:oule,
G . "' . •( 1 962)
?he induction
of ovulat ioni n ano e s trous