CAPÍTULO III: SUSTENTACIÓN
3.3. Ideas centrales por pieza
H. pylori and E. coli strains used in this study, and their relevant characteristics
are shown in Table 2.1. H. pylori was identified by morphology, Gram
staining and urease testing. The strains used in this study were isolated and
typed by myself, J. Hale, J. Rhead, S. Patel and A. Memon. Table 2.2 shows
the details of the plasmids used in this study and their relevant
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Table 2.1 Bacterial strains
Strain Relevant characteristics Reference/Source
N XG Z Electrocompetent cells F- mcrA mrr-hsdRMS-mcrBC) A A ) M X D - U K L G - tonA Novagen
60190 Wild-type, VacA type s1/m1, Tox+ Leunk et al. (1988) 60190/A130 As 60190. except vacA::km Cover et al, 1994 60190 pCTB2::cat pBluescript containing 3 cysS to vacA nt 274 from strain
60190. cat inserted into Hind III site immediately 3 to cysS ; AmpR; CmR
Letley et al. (2003)
60190 P(-6)G As 60190 pCTB2::cat except proline replaced with glycine at position -6 of the signal sequence.
D.P. Letley
60190 S(-3)L As 60190 pCTB2::cat except serine replaced with leucine at position -3 of the signal sequence.
D.P. Letley
60190 P(-6)GS(-3)L As 60190 pCTB2::cat except proline replaced with glycine at position -6, and serine replaced with leucine at position -3 of the signal sequence.
D.P. Letley
Tx30a Wild-type, VacA type s2/m2, Tox- Leunk et al. (1988) Tx30a pA153::cat As Tx30a but cat cysS; CmR Letley et al. (2003) Tx30a G(-6)P As Tx30a pA153::cat except glycine replaced with proline at
position -6 of the signal sequence.
D.P. Letley
Tx30a L(-3)S As Tx30a pA153::cat except leucine replaced with serine at position -6 of the signal sequence.
D.P. Letley
Tx30a G(-6)PL(-3)S As Tx30a pA153::cat except glycine replaced with proline at position -6, and leucine replaced with serine at position -3 of the signal sequence.
D.P. Letley
60190 G(-6)PL(-3)S As Tx30a G(-6)PL(-3)S but with plasmid transformed into a 60190 strain background.
D.P. Letley and C.G. Masters (This study). Tx30a P(-6)GS(-3)L As 60190 P(-6)GS(-3)L but with plasmid transformed into a
Tx30a strain background.
D.P. Letley and C.G. Masters (This study). 60190/ SL As pCTB2::cat except stem loop deleted from vacA nt 608 to
704 and replaced with a Bgl II site; AmpR; CmR
G.L. Narayanan
60190/SL GtoA As 60190 pCTB2::cat except with G at position +28 within the vacA UTR A
This study
60190/IRDIS As 60190 pCTB2::cat except with a 6bp disruption in an
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motif of the vacA promoter.
This study
45B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA +.
This study
46B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA -.
This study
72B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA +.
This study
73B Clinical strain isolated from corpus. vacA type s1/m2/i2,
cagA +.
This study
77B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA +.
This study
83B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA +.
This study
86B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA +.
This study
93B Clinical strain isolated from corpus. vacA type s1/m2/i1,
cagA
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120B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA +.
This study
121B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA +.
This study
122B Clinical strain isolated from corpus. vacA type s1/m1/i2,
cagA +.
This study
124B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA +.
This study
126B Clinical strain isolated from corpus. vacA type s1/m2/i1,
cagA +.
This study
173B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA +.
This study
181B Clinical strain isolated from corpus. vacA type s1/m1/m2/i2,
cagA -.
This study
182B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA -.
This study
187B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA +.
This study
192B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA +.
This study
194B Clinical strain isolated from corpus. vacA type s1/m2/i1,
cagA -.
This study
201B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA +.
This study
206B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA +.
This study
215B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA +.
This study
218B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA +.
This study
221B Clinical strain isolated from corpus. vacA type s1/m2/i2,
cagA -.
This study
223B Clinical strain isolated from corpus. vacA type s2/m2/i2,
cagA -.
This study
233B Clinical strain isolated from corpus. vacA type s2/m2/i2,
cagA +.
This study
240B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA +.
This study
242B Clinical strain isolated from corpus. vacA type s2/m2/i2,
cagA -.
This study
247B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA +.
This study
249B Clinical strain isolated from corpus. vacA type s1/m2/i1/i2,
cagA +.
This study
253B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA +.
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255B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA +.
This study
256B Clinical strain isolated from corpus. vacA type s2/m2/i2,
cagA +.
This study
265B Clinical strain isolated from corpus. vacA type s1/m2/i2,
cagA +.
This study
271B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA -.
This study
274B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA +.
This study
308B Clinical strain isolated from corpus. vacA type s1/m2/i2,
cagA -.
This study
320B Clinical strain isolated from corpus. vacA type s1/m2/i2,
cagA -.
This study
322B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA +.
This study
326B Clinical strain isolated from corpus. vacA type s1/i2/m2,
cagA -.
This study
335B Clinical strain isolated from corpus. vacA type s1/m1/i1,
cagA +.
This study
350B Clinical strain isolated from corpus. vacA type s1/m2/i1,
cagA +.
This study
Table 2.2 Plasmids
Name Construct Resistance Reference/Source
pCTB2::cat pBluescript containing 3
cysS to vacA nt 274 from
strain 60190. cat inserted into Hind III site
immediately 3 to cysS ; AmpR; CmR Ampicillin, chloramphenicol Letley et al, 2000 pCTB2::cat P(-6)GS(-3)L As 60190 pCTB2::cat except proline replaced with glycine at position -6, and serine replaced with leucine at position -3 of the signal sequence.
Ampicillin, chloramphenicol
Letley et al, 2000
pCTB2::cat SL As pCTB2::cat except stem loop deleted from vacA nt 608 to 704 and replaced with a Bgl II site; AmpR; CmR.
Ampicillin, chloramphenicol
G.L. Narayanan 2005
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