Inferencia filogenética

This Appendix contains individual circos plots for the first 25 nodes. Genes and

nucleotide identities can be either of Raoultella sp. (green), bacterial (non-Raoultella)

(pink), uncharacterised (black), or eukaryotic (orange) in origin. (Refer to page 48

Figure 23 for how to read circos plot information)

Figure 35. Individual circos plot of node 1. The nucleotide identity is that of Raoultella

(green). There are very few non-coding regions, and most genes identify as Raoultella (green),

or bacterial (pink) in origin with a few uncharacterised genes (black). The tagged genes are

mostly associated with resistance and virulence factors. Node ratio 1:1000 (1 = 1000 bps).

Figure 36. Individual circos plot of node 2. The nucleotide identity is that of Raoultella

(green). There are many scattered small non-coding regions, and most genes identify as

Raoultella (green), or bacterial (pink) in origin with a few uncharacterised genes (black). The

tagged genes are mostly associated with resistance, virulence factors and type IV secretion.

There are several clusters that identify with other bacteria. Node ratio 1:1000 (1 = 1000 bps).

Figure 37. Individual circos plot of node 3. The nucleotide identity is that of Raoultella

(green). There are scattered small non-coding regions, and most genes identify as Raoultella

(green), or bacterial (pink) in origin with a few uncharacterised genes (black). The tagged genes

Figure 38. Individual circos plot of node 4. The nucleotide identity is that of Raoultella

(green). There are scattered small non-coding regions, and most genes identify as Raoultella

(green), or bacterial (pink) in origin with a few uncharacterised genes (black). The tagged genes

are mostly associated with polysaccharide synthesis, and resistance. Of particular note is the

cluster of cellulose synthesis related genes in the region between 187,000 and 212,000. Node

ratio 1:1000 (1 = 1000 bps).

Figure 39. Individual circos plot of node 5. The nucleotide identity is that of Raoultella

(green). There are scattered non-coding regions, and most genes identify as Raoultella (green),

or bacterial (pink) in origin with a few uncharacterised genes (black). The tagged genes are

mostly associated with secretion systems and enzymes. Of particular note is the cluster of Type

II secretion system and pullulanase genes in the region between 60,000 and 75,000. Node ratio

1:1000 (1 = 1000 bps).

Figure 40. Individual circos plot of node 6. The nucleotide identity is that of Raoultella

(green). There are scattered non-coding regions, and most genes identify as Raoultella (green),

or bacterial (pink) in origin with a few uncharacterised genes (black), and a single gene of

eukaryotic (orange) origin. The tagged genes are mostly associated with efflux and pili. Node

ratio 1:1000 (1 = 1000 bps).

Figure 41. Individual circos plot of node 7. The nucleotide identity is that of Raoultella

(green). There are scattered non-coding regions, and most genes identify as Raoultella (green),

or bacterial (pink) in origin with a few uncharacterised genes (black). The tagged genes are

mostly associated with phage, and enzymes. There are multiple clusters of bacterial (pink)

origin. Node ratio 1:1000 (1 = 1000 bps).

Figure 42. Individual circos plot of node 8. The nucleotide identity is that of Raoultella

(green). There are scattered non-coding regions, and most genes identify as Raoultella (green),

or bacterial (pink) in origin with a few uncharacterised genes (black). The tagged genes are

mostly associated with fimbriae, and resistance. Node ratio 1:1000 (1 = 1000 bps).

Figure 43. Individual circos plot of node 9. The nucleotide identity is that of Raoultella

(green). There are scattered non-coding regions, and most genes identify as Raoultella (green),

or bacterial (pink) in origin with a few uncharacterised genes (black). The tagged genes are

mostly associated with quorum sensing, and resistance. Node ratio 1:1000 (1 = 1000 bps).

Figure 44. Individual circos plot of node 10. The nucleotide identity is that of Raoultella

(green). There are scattered non-coding regions, and most genes identify as Raoultella (green),

or bacterial (pink) in origin with a few uncharacterised genes (black). The tagged genes are

mostly associated with fimbriae and type IV secretion systems. Node ratio 1:1000 (1 = 1000

bps).

Figure 45. Individual circos plot of node 11. The nucleotide identity is that of Raoultella

(green). There are scattered non-coding regions, and most genes identify as Raoultella (green),

or bacterial (pink) in origin with a few uncharacterised genes (black). The tagged genes are

mostly associated with resistance and virulence. Node ratio 1:1000 (1 = 1000 bps).

Figure 46. Individual circos plot of node 12. The nucleotide identity is that of Raoultella

(green). There are scattered non-coding regions, and most genes identify as Raoultella (green),

or bacterial (pink) in origin with a few uncharacterised genes (black). The tagged gene is

associated with resistance. Node ratio 1:1000 (1 = 1000 bps).

Figure 47. Individual circos plot of node 13. The nucleotide identity is that of Raoultella

(green). There are scattered non-coding regions, and most genes identify as Raoultella (green),

or bacterial (pink) in origin. The tagged genes are associated with resistance. Node ratio 1:1000

(1 = 1000 bps).

Figure 48. Individual circos plot of node 14. The nucleotide identity is that of Raoultella

(green). There are scattered non-coding regions, and most genes identify as Raoultella (green),

or bacterial (pink) in origin. The tagged gene is associated with phage. Node ratio 1:1000 (1 =

1000 bps).

Figure 49. Individual circos plot of node 15. The nucleotide identity is that of Raoultella

(green). There are scattered non-coding regions, and most genes identify as Raoultella (green),

or bacterial (pink) in origin with a few uncharacterised genes (black). The tagged gene is

associated with fimbriae. Node ratio 1:1000 (1 = 1000 bps).

Figure 50. Individual circos plot of node 16. The nucleotide identity is that of Raoultella

(green). There are scattered non-coding regions, and most genes identify as Raoultella (green),

or bacterial (pink) in origin. The tagged gene is associated with toxins. Node ratio 1:1 (1 = 1

bp).

Figure 51. Individual circos plot of node 17. The nucleotide identity is that of Raoultella

(green). There are scattered non-coding regions, and most genes identify as Raoultella (green),

or bacterial (pink) in origin with an uncharacterised gene (black), and a gene of eukaryotic

origin (orange). The tagged genes are associated with resistance. Node ratio 1:1 (1 = 1 bp).

Figure 52. Individual circos plot of node 18. The nucleotide identity is that of Raoultella

(green). There are scattered non-coding regions, and most genes identify as Raoultella (green),

or bacterial (pink) in origin. The tagged genes are associated with resistance and

polysaccharides. Node ratio 1:1 (1 = 1 bp).

Figure 53. Individual circos plot of node 19. The nucleotide identity is that of Raoultella

(green). There are scattered non-coding regions, and most genes identify as Raoultella (green),

or bacterial (pink) in origin with a few uncharacterised genes (black). The tagged genes are

associated with resistance and phage. With around a third of only bacterial (pink) gene origin.

Node ratio 1:1 (1 = 1 bp).

Figure 54. Individual circos plot of node 20. The nucleotide identity is that of Raoultella

(green). There are scattered non-coding regions, and most genes identify as Raoultella (green),

Figure 55. Individual circos plot of node 21. The nucleotide identity is that of Raoultella

(green). There are scattered non-coding regions, and most genes identify as Raoultella (green),

or bacterial (pink) in origin. The tagged gene is associated with virulence. Node ratio 1:1 (1 = 1

bp).

Figure 56. Individual circos plot of node 22. The nucleotide identity is that of Raoultella

(green). There are scattered non-coding regions, and most genes identify as Raoultella (green),

Figure 57. Individual circos plot of node 23. The nucleotide identity is that of Raoultella

(green). There are scattered non-coding regions, and most genes identify as Raoultella (green),

or bacterial (pink) in origin. The tagged gene is associated with resistance. Node ratio 1:1 (1 = 1

bp).

Figure 58. Individual circos plot of node 24. The nucleotide identity is that of Raoultella

(green). There are scattered non-coding regions, and most genes identify as Raoultella (green),

Figure 59. Individual circos plot of node 25. The nucleotide identity is of bacteria (non –

Raoultella)(pink). There are scattered non-coding regions, and most genes identify as bacterial

(pink) in origin with a few Raoultella (green), and an uncharacterised (black). The tagged genes

In document Caracterización molecular y fenotípica de aislamientos clínicos y ambientales del complejo pseudallescheria boydii (página 47-51)