Course director: DR.GYÖRGYI HORVÁTH, associate professor
Department of Pharmacognosy
2 credit ▪ midsemester grade ▪ Optional module ▪ spring semester ▪ recommended semester: 4
Number of hours/semester: 26 lectures + 0 practices + 2 seminars = total of 28 hours
Course headcount limitations (min-max.): min. 5 – max. 20
Prerequisites: OAA-OR1 completed + OAA-OR2 completed
Topic
With the increasing demand for herbal medicinal products, nutraceuticals, and natural products for health care all over the world, medicinal plant extract manufacturers and essential oil producers have started using the most appropriate extraction and chromatographic technologies in order to produce and standardize extracts and essential oils of defined quality. The aim of this course is to introduce the most important extraction methods and chromatographic techniques used in the research of medicinal plants. Students should be able to apply the acquired knowledge in their medical or pharmaceutical practice.
Task: To introduce state-of-the-art extraction techniques (maceration, infusion, digestion, decoction, percolation, Soxhlet, ultrasound extraction, supercritical fluid extraction, water and steam distillation, enfleurage, expression) and chromatographic analyses (column chromatography, thin layer chromatography, high pressure liquid chromatography) of medicinal plants and their role in the production of herbal medicines. Some applications, relating to different classes of substances, are presented to demonstrate the versatility of various chromatographic techniques.
Conditions for acceptance of the semester
Criteria of acceptance of the semester: Satisfactory fulfilment of 1 written test based on the lectures. Students have to give a short oral presentation based on 1 article read by themselves. Absences: according to the Code of Studies and Examinations of the Medical School
Requirements: students have to know the most frequent extraction and analytical techniques used in medicinal and aromatic plant research.
Making up for missed classes
Individual consultation is possible.
Reading material
Recommended literature:
1. Handa S.S., Khanuja S.P.S., Longo G., Rakesh D.D.: Extraction Technologies for Medicinal and Aromatic Plants, International Centre for Science and High Technology, Trieste, 2008
2. Reich E., Schibli A.: High-Performance Thin-Layer Chromatography for the Analysis of Medicinal Plants, Thieme, UK, 2007 3. Colegate S.M.: Bioactive Natural Products Detection Isolation and Structural Determination, CRC Press, 2008
4. Niessen W.M.A.: Liquid Chromatography - Mass Spectrometry, CRC Press, 2006 5. Nyiredy, Sz.: Planar Chromatography, Springer, Budapest, 2001.
6. Harris, DC.: Quantitative Chemical Analysis, WH. Freeman and Co., 7th edition, New York, 2007,
7. Schiedt K., Liaaen-Jensen S.: Isolation and Analysis, in Carotenoids Vol. 1A: Isolation and Analysis, Eds.: Britton G., Liaaen-Jensen S., Pfander H., Birkhäuser Verlag, Basel, 1995.
Lectures
1 Introduction of the course requirements. An Overview of Extraction Techniques for Medicinal and Aromatic Plants. Dr. Horváth Györgyi
2 Introduction of state-of-the-art extraction techniques: maceration, infusion, digestion, decoction, percolation, Soxhlet, ultrasound extraction, supercritical fluid extraction, water and steam distillation, enfleurage, expression.
Dr. Horváth Györgyi
3 Introduction to Analytical Separations. Dr. Benkő András Antal
4 Techniques for the Purposes of Botanical Research. Dr. Benkő András Antal
5 Planar Chromatography: TLC. Dr. Benkő András Antal
6 Role of Different Procedures and Test Systems in the Knowledge of Herbal Substances. Dr. Benkő András Antal
7 Column Chromatography Systems: GC. Dr. Benkő András Antal
8 Column Chromatography Systems: HPLC. Dr. Benkő András Antal
9 Detectors Used in Different Separation Techniques and their Role in Qualitative and Quantitative Analysis. Dr. Benkő András Antal
10 Classification of Detectors Based on their Sensitivity and Selectivity. Dr. Benkő András Antal
11 Role of thin layer chromatography (TLC) in detecting microbiological processes: TLC-Bioautography. Dr. Horváth Györgyi
12 Antimicrobial activity of essential oils using TLC-Bioautography. Dr. Horváth Györgyi
13 Isolation and identification of carotenoids from medicinal plants. Dr. Molnár Péter
14 Role of column liquid chromatography (CLC) in carotenoid research. Dr. Molnár Péter
15 Extraction, separation and detection of carbohydrates from various plant samples, applying diverse chromatographic techniques.
Dr. Farkas Ágnes
16 Extraction, separation and detection of carbohydrates from various plant samples, applying diverse chromatographic techniques.
Dr. Farkas Ágnes
17 Identification of specific metabolites in various herbal secretions and their processed products. Dr. Farkas Ágnes
18 Identification of specific metabolites in various herbal secretions and their processed products. Dr. Farkas Ágnes
19 Isolation and Identification of flavonoids in medicinal plants. Dr. Papp Nóra
20 Isolation and Identification of flavonoids in medicinal plants. Dr. Papp Nóra
21 Comparative phytochemical studies on populations of medicinal plant taxa; their role in plant identification and in pharmacy. Dr. Papp Nóra
22 Comparative phytochemical studies on populations of medicinal plant taxa; their role in plant identification and in pharmacy. Dr. Papp Nóra
23 Student’s lecture. Dr. Benkő András Antal 24 Student’s lecture. Dr. Benkő András Antal
25 Written test.
Dr. Benkő András Antal
26 Written test.
Dr. Benkő András Antal
Practices Seminars
1 Chromatographic seminar: GC. 2 Chromatographic seminar: HPLC.
Exam topics/questions
The topics of the 1 written test are similar to the topics of the lectures.
Participants
184
OAF-GTO E
NVIRONMENTALG
ENOTOXICOLOGY ANDE
COTOXICOLOGYCourse director: DR.CSABA VARGA, associate professor
Department of Public Health Medicine
2 credit ▪ midsemester grade ▪ Optional module ▪ spring semester ▪ recommended semester: 4
Number of hours/semester: 20 lectures + 8 practices + 0 seminars = total of 28 hours
Course headcount limitations (min-max.): min. 5 – max. 15
Prerequisites: OAA-MB2 completed
Topic
Short description of the course: The objective of this interdisciplinary field is to demonstrate the environmental effects of the different chemical agents and their consequences and the strategy of the research. The main educational task of the subject: The course provides an up-to-date preventive approach including the role of environmental genotoxic effects as a cause of increasing frequency of cancers and congenital malformations.
Conditions for acceptance of the semester
Maximum of 15 % absence allowed
Making up for missed classes Reading material
Hulka BS, Wilcosky TC, Griffith JC.: Biological Markers in Epidemiology, Oxford University Press, 1990
Fairbairn DW, Olive PL, O’Neil KL: The Comet Assay: a Comprehensive Review, Mutation Res., 1995 339: 37-59. Venitt S, Parry JM (eds.): Mutagenicity Testing, IRL Press Ltd, Oxford, 1984
Kirkland, D.: Report on the International Workshop on Standardisation of Genotoxicity Test Procedures
Maron, DM, Ames BN: Revised Methods for the Salmonella Mutagenicity Test, Mutation Res., 1983 113, 173-215
Lectures
1 Environmental mutagens.
Dr. Varga Csaba
2 Genotoxic carcinogens. Dr. Varga Csaba
3 DNA primary lesions, levels of mutations I. Dr. Szendi Katalin
4 DNA primary lesions, levels of mutations II. Dr. Szendi Katalin
5 Consequences of mutations. Dr. Varga Csaba
6 General criteria for genotoxicity tests. Dr. Varga Csaba
7 Genotoxicological strategies. I. Dr. Szendi Katalin
8 Genotoxicological strategies. II. Dr. Szendi Katalin
9 Genotoxicity in details I. Dr. Varga Csaba 10 Genotoxicity in details II.
Dr. Varga Csaba
11 Conventional tests: DNA adduct measurements, UDS, Ames test, HPRT in cell cultures, chromosomal aberrations, SCE, micronucleus. I.
Dr. Szendi Katalin
12 Conventional tests: DNA adduct measurements, UDS, Ames test, HPRT in cell cultures, chromosomal aberrations, SCE, micronucleus. II.
Dr. Szendi Katalin
13 Molecular methods: transgenic animals, single cell DNA microgel electrophoresis I. Dr. Varga Csaba
14 Molecular methods: transgenic animals, single cell DNA microgel electrophoresis II. Dr. Varga Csaba
15 Image analysis and computer aided evaluation I. Dr. Szendi Katalin
16 Image analysis and computer aided evaluation II. Dr. Szendi Katalin
17 Genotoxicological endpoints as biomarkers I. Dr. Varga Csaba
18 Genotoxicological endpoints as biomarkers II. Dr. Varga Csaba
19 Individual risk assessment I. Dr. Szendi Katalin
20 Individual risk assessment I. Individual risk assessment II. Dr. Szendi Katalin
Practices
1 Bacterial mutagenicity studies I. 2 Bacterial mutagenicity studies II. 3 Cytogenetic studies I.
4 Cytogenetic studies II. 5 Comet assay I. 6 Comet assay II. 7 Biomarker studies I. 8 Biomarker studies II.
Seminars
Exam topics/questions
Questions for the exam: 1. Environmental mutagens. 2. Genotoxic carcinogens. 3. DNA lesions.
4. Levels of mutations. 5. Consequences of mutations.
6. General criteria for genotoxicity tests. 7. Genotoxicological strategies.
8. Conventional tests: DNA adduct measurements, UDS, Ames test, HPRT in cell cultures,. 9. Chromosomal aberrations, SCE, micronucleus.
10. Molecular methods: transgenic animals.
11. Molecular methods: single cell DNA microgel electrophoresis. 12. Genotoxicological endpoints as biomarkers.
13. Individual risk assessment. 14. Bacterial mutagenicity studies 15. Cytogenetic studies
16. Comet assay
Participants
186
OAF-JAV S
IGNALT
RANSDUCTIONCourse director: DR.TÍMEA BERKI, professor
Department of Immunology and Biotechnology
2 credit ▪ midsemester grade ▪ Optional module ▪ spring semester ▪ recommended semester: 4
Number of hours/semester: 28 lectures + 0 practices + 0 seminars = total of 28 hours
Course headcount limitations (min-max.): min. 5 – max. 20
Prerequisites: OAA-IMM parallel
Topic
Novel antibodies against receptors, protein kinase inhibitors, and antisense oligonucleotides targeting both signal transduction and gene expression will predominate the therapeutic approaches in the coming decades. The course focus on the therapeutic potential for targeting cell signaling mechanisms with particular attention to cancer therapies and inflammatory signaling pathways as well as immunomodulation. General concepts of inter-and intracellular signal transduction: receptor-mediated signal transduction, cell surface receptors, steroid hormone and nuclear receptors and their cytoplasmic signal transduction and nuclear responses, apoptotic cell signaling will be discussed together with the novel approaches to drug discovery in signal transduction. Internationally known experts of the field guest lecturers from Hungarian universities are contributing in the course.
Conditions for acceptance of the semester
Maximum 3 absences allowed
Making up for missed classes
-
Reading material
1. Gerhard Krauss: Biochemistry of Signal Transduction and Regulation, Finkel, Toren (ed.), Wiley 2. Gutkind, J. Silvio (ed.): Signal Transduction and Human Disease, John Wiley & Sons, Inc.
3. Frederick Marcus: Bioinformatics and Systems Biology: Collaborative Research and Resources, Springer, 2008
Lectures
1 Introduction, overlap with other disciplines Dr. Berki Timea
2 Overview of extracellular signaling Dr. Berki Timea
3 Families of extracellular receptors Dr. Boldizsár Ferenc
4 Second messengers (cAMP) Dr. Boldizsár Ferenc 5 Receptor tyrosine kinases
Dr. Boldizsár Ferenc 6 The Ca++ signal
Dr. Boldizsár Ferenc 7 Transcription factors
Dr. Berki Timea
8 Signaling in the specific immune system: B cell signaling. Dr. Berki Timea
9 T cell activation and signaling Dr. Berki Timea
10 Fcgamma and Fcepsilon Receptor signaling Dr. Berki Timea
11 Tyrosine kinase-linked receptors I.: cytokine/chemokine signaling Dr. Boldizsár Ferenc
12 Tyrosine kinase-linked receptors II.: growth factors Dr. Boldizsár Ferenc
13 Receptors with intrinsic enzymatic activity (insulin, growth factors). Dr. Berki Timea
14 G-protein-linked receptors (epinephrine, serotonin, glucagon). Dr. Berki Timea
15 Signaling in tumor cells (EGF-R, Her-2R, adhesion molecules). Dr. Berki Timea
16 Apoptosis signaling Dr. Berki Timea
17 Ion-channel receptors (acetilcholine receptor). Cell-surface hormone receptors Dr. Berki Timea
18 Intracellular/nuclear receptor signaling (steroid hormones and thyroxin). Dr. Boldizsár Ferenc
19 Non-genomic steroid hormone signaling pathways. Dr. Boldizsár Ferenc
20 Signaling in the innate immune system: CR and TLR signaling Dr. Berki Timea
21 Receptor interactions, signaling cross-talk Dr. Berki Timea
22 Wnt receptor signaling. Dr. Pongrácz Judit
23 Role of signal transduction in cell differentiation Dr. Pongrácz Judit
24 Adhesion molecule signaling Dr. Berki Timea
25 Role of adhesion molecules in tumor progression Dr. Berki Timea
26 Signaling in the nervous system Dr. Berki Timea
27 Pharmacological influence of the signaling. Dr. Pethő Gábor
28 Pharmacological influence of the signaling. Dr. Pethő Gábor Practices Seminars Exam topics/questions - Participants
188